<abstract xmlns="http://www.w3.org/1999/xhtml">

The aim of this study is to present the effect of treatment with ureolytic bacteria (<italic>Sporosarcina pasteurii</italic>) on the compressibility parameters of mineral and anthropogenic soils. In the presence of the urease enzyme, secreted by a strain of <italic>Sporosarcina pasteurii</italic> bacteria, urea hydrolysis occurs, allowing CaCO3 to be precipitated. The literature suggests applying the Microbially Induced Calcite Precipitation (MICP) method to non-cohesive soils. In order to determine whether the biomineralization process occurs in other soil types, cohesive and anthropogenic soils were tested in the laboratory. Compressibility tests were carried out in the laboratory on MICP-treated and untreated soils as reference samples. The process of biocementation in the soil is made possible by the introduction of bacteria into the soil and subsequent activation by a cementation solution (consisting of urea and calcium ions Ca2+). This paper presents the methodology for introducing bacteria into the soil, as well as the effect of the biomineralization process on the deformation parameters of the tested materials.
</abstract>

The aim of this study is to present the effect of treatment with ureolytic bacteria (Sporosarcina pasteurii) on the compressibility parameters of mineral and anthropogenic soils. In the presence of the urease enzyme, secreted by a strain of Sporosarcina pasteurii bacteria, urea hydrolysis occurs, allowing CaCO3 to be precipitated. The literature suggests applying the Microbially Induced Calcite Precipitation (MICP) method to non-cohesive soils. In order to determine whether the biomineralization process occurs in other soil types, cohesive and anthropogenic soils were tested in the laboratory. Compressibility tests were carried out in the laboratory on MICP-treated and untreated soils as reference samples. The process of biocementation in the soil is made possible by the introduction of bacteria into the soil and subsequent activation by a cementation solution (consisting of urea and calcium ions Ca2+). This paper presents the methodology for introducing bacteria into the soil, as well as the effect of the biomineralization process on the deformation parameters of the tested materials.

Effect of Ureolytic Bacteria on Compressibility of the Soils with Variable Gradation

Faculty of Civil Engineering and Environmental Sciences

Architecture, Civil Engineering, Environment

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The aim of this study is to present the effect of treatment with ureolytic bacteria (Sporosarcina pasteurii) on the compressibility parameters of mineral and...

Material	D₅₀ (mm)	ρ_s (g/cm³)	I_p (%)
Silt (Si)	0.031	2.67	7.7
Fine sand (FSa)	1.90	2.65	–
Fly ash (FA)	0.060	2.18	–

Phase-mineral composition (%)		Chemical composition (%)
SiO₂	43.28	Si	22.37
Al₂O₃	31.81	Al	15.80
Fe₂O₃	1.82	C	3.49
CaO	1.10	K	2.46
MgO	2.16	Fe	1.87
K₂O	3.42	Mg	0.97
TiO₂	0.63	Ca	0.81
LOI	12.45	Ti	0.56

Effect of Ureolytic Bacteria on Compressibility of the Soils with Variable Gradation

Published Online: Oct 20, 2023

Page range: 131 - 139

Received: Apr 05, 2023

Accepted: Apr 25, 2023

DOI: https://doi.org/10.2478/acee-2023-0040

Keywords
Biomineralization, MICP, Soil improvement, Soil parameters, Ureolytic bacteria

© 2023 Mariola Wasil et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

The parameters of tested soils

Chemical and phase composition of tested fly ash (FA)

Effect of Ureolytic Bacteria on Compressibility of the Soils with Variable Gradation

Published Online: Oct 20, 2023

Page range: 131 - 139

Received: Apr 05, 2023

Accepted: Apr 25, 2023

DOI: https://doi.org/10.2478/acee-2023-0040

KeywordsBiomineralization, MICP, Soil improvement, Soil parameters, Ureolytic bacteria

© 2023 Mariola Wasil et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

The parameters of tested soils

Chemical and phase composition of tested fly ash (FA)

Keywords
Biomineralization, MICP, Soil improvement, Soil parameters, Ureolytic bacteria