<abstract xmlns="http://www.w3.org/1999/xhtml">

The genes considered in this study, namely, <italic>LYN, CCL2, ITGB3</italic> and <italic>IL6</italic> can be related to immune response in porcine buccal mucosa cells primary cultured <italic>in vitro</italic>. These genes are also responsible for, inter alia, cell migration, differentiation, proliferation and apoptosis, intracellular signal transduction, extracellular matrix binding and wound healing. A total of 20 pubertal crossbred Landrace gilts bred on commercial farms were used to obtain buccal mucosa cell cultures, which were harvested on the 7th, 15th and 30th day after initiation of the culture. Expression levels of <italic>LYN, CCL2, ITGB3</italic> and <italic>IL6</italic> were evaluated employing Real-Time Quantitative Polymerase Chain Reaction. All studied genes showed expression. The expression of <italic>CCL2</italic> on day 15 was the highest of all factors measured. The greatest difference between the measurements occurred in gene <italic>IL6,</italic> between 7th and 15th day, while the least difference between the measurements occurred in gene <italic>LYN</italic>, between 7th and 15th day. Moreover, on the 7th day, <italic>LYN</italic> presented the lowest expression among all studied genes. Although <italic>in vitro</italic> conditions are much more controlled than <italic>in vivo</italic> conditions, all the factors that may positively or negatively affect cultured cells still cannot be fully predicted. Nevertheless, <italic>LYN, CCL2, ITGB3</italic> and <italic>IL6</italic> are a valuable starting point for studying further immunological processes in oral mucosal epithelial cells. Given their high regenerative potential, research into them is a highly valuable source of information for future wound healing therapies, where immunological processes should be carefully considered.
</abstract>

The genes considered in this study, namely, LYN, CCL2, ITGB3 and IL6 can be related to immune response in porcine buccal mucosa cells primary cultured in vitro. These genes are also responsible for, inter alia, cell migration, differentiation, proliferation and apoptosis, intracellular signal transduction, extracellular matrix binding and wound healing. A total of 20 pubertal crossbred Landrace gilts bred on commercial farms were used to obtain buccal mucosa cell cultures, which were harvested on the 7th, 15th and 30th day after initiation of the culture. Expression levels of LYN, CCL2, ITGB3 and IL6 were evaluated employing Real-Time Quantitative Polymerase Chain Reaction. All studied genes showed expression. The expression of CCL2 on day 15 was the highest of all factors measured. The greatest difference between the measurements occurred in gene IL6, between 7th and 15th day, while the least difference between the measurements occurred in gene LYN, between 7th and 15th day. Moreover, on the 7th day, LYN presented the lowest expression among all studied genes. Although in vitro conditions are much more controlled than in vivo conditions, all the factors that may positively or negatively affect cultured cells still cannot be fully predicted. Nevertheless, LYN, CCL2, ITGB3 and IL6 are a valuable starting point for studying further immunological processes in oral mucosal epithelial cells. Given their high regenerative potential, research into them is a highly valuable source of information for future wound healing therapies, where immunological processes should be carefully considered.

Potential Role of LYN, CCL2, ITGB3 and IL6 Genes in the Immune Response of Porcine Buccal Mucosa Cells

Medical Journal of Cell Biology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The genes considered in this study, namely, LYN, CCL2, ITGB3 and IL6 can be related to immune response in porcine buccal mucosa cells primary cultured in...

Potential Role of LYN, CCL2, ITGB3 and IL6 Genes in the Immune Response of Porcine Buccal Mucosa Cells

Published Online: Jul 09, 2022

Page range: 49 - 55

Received: Apr 16, 2022

Accepted: Jun 02, 2022

DOI: https://doi.org/10.2478/acb-2022-0008

Keywords
immune response, porcine buccal mucosa, in vitro culture, RT-qPCR

© 2022 Blanka Borowiec et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Potential Role of LYN, CCL2, ITGB3 and IL6 Genes in the Immune Response of Porcine Buccal Mucosa Cells

Published Online: Jul 09, 2022

Page range: 49 - 55

Received: Apr 16, 2022

Accepted: Jun 02, 2022

DOI: https://doi.org/10.2478/acb-2022-0008

Keywordsimmune response, porcine buccal mucosa, in vitro culture, RT-qPCR

© 2022 Blanka Borowiec et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
immune response, porcine buccal mucosa, in vitro culture, RT-qPCR