TCCCTTCACCTACATTTTCTG | TGCCTGCCTCGCCACATC | 89 | |
CCATCGGTGCCAAGACAGG | GGGGAGGTGGTGTTAGGAG | 164 | |
CCCTCGCCATCATTCACTG | TTCTCCCTCTTCCCGCTGT | 146 |
Preincubation | 95 | 600 | 1 | |
Amplification | Denaturation | 95 | 15 | 40 |
Annealing | 58 | 15 | ||
Elongation | 72 | 15 | ||
Melting | 95 | 60 | 1 | |
40 | 60 | |||
70 | 1 | |||
95 | 1 | |||
Cooling | 37 | 30 | 1 |