<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Sperm separation for ICSI is an essential step in realization of the IVF procedures. The method of microfluidic separation of sperm cells using chips has been applied more and more frequently in recent years. This method is often presented as extremely gentle to spermatozoa and decreasing significantly concentration of sperm cells with fragmented DNA when compared to conventional methods. The aim of our study was to verify a microfluidic chip system from the perspective of its potential to select spermatozoa with non-fragmented DNA. We tested the efficiency of this separation method against the swim-up method. In this study we evaluated sperm DNA integrity before and after the separation methods in ten patients. Ejaculate of each patient was separated by both the swim up method and the microfluidic chip method at the same time. It was shown that both the methods are very similar in reduction of spermatozoa with fragmented DNA. Interestingly, the concentration of spermatozoa with fragmented DNA was lower after the microfluidic separation than after the swim-up method in all the patients. Nevertheless, the differences were not statistically significant with only 2.1% on average, which is negligible in terms of practical use.</p><p><bold>Running title</bold>: Microfluidic chip and DNA fragmentation</p></abstract>

Sperm separation for ICSI is an essential step in realization of the IVF procedures. The method of microfluidic separation of sperm cells using chips has been applied more and more frequently in recent years. This method is often presented as extremely gentle to spermatozoa and decreasing significantly concentration of sperm cells with fragmented DNA when compared to conventional methods. The aim of our study was to verify a microfluidic chip system from the perspective of its potential to select spermatozoa with non-fragmented DNA. We tested the efficiency of this separation method against the swim-up method. In this study we evaluated sperm DNA integrity before and after the separation methods in ten patients. Ejaculate of each patient was separated by both the swim up method and the microfluidic chip method at the same time. It was shown that both the methods are very similar in reduction of spermatozoa with fragmented DNA. Interestingly, the concentration of spermatozoa with fragmented DNA was lower after the microfluidic separation than after the swim-up method in all the patients. Nevertheless, the differences were not statistically significant with only 2.1% on average, which is negligible in terms of practical use.Running title: Microfluidic chip and DNA fragmentation

Comparison of microfluidic and swim-up sperm separation methods for IVF

Medical Journal of Cell Biology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Comparison of microfluidic and swim-up sperm separation methods for IVF"}

Sperm separation for ICSI is an essential step in realization of the IVF procedures. The method of microfluidic separation of sperm cells using chips has been...

Comparison of microfluidic and swim-up sperm separation methods for IVF

Published Online: Dec 31, 2020

Page range: 170 - 175

Received: Oct 27, 2020

Accepted: Dec 05, 2020

DOI: https://doi.org/10.2478/acb-2020-0022

Keywords
spermatozoa, microfluidic, DNA integrity, swim-up, sperm preparation

© 2020 Michal Ješeta, Kateřina Franzová, Jana Žáková, Pavel Ventruba, Igor Crha, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Comparison of microfluidic and swim-up sperm separation methods for IVF

Published Online: Dec 31, 2020

Page range: 170 - 175

Received: Oct 27, 2020

Accepted: Dec 05, 2020

DOI: https://doi.org/10.2478/acb-2020-0022

Keywordsspermatozoa, microfluidic, DNA integrity, swim-up, sperm preparation

© 2020 Michal Ješeta, Kateřina Franzová, Jana Žáková, Pavel Ventruba, Igor Crha, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
spermatozoa, microfluidic, DNA integrity, swim-up, sperm preparation