<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The aim of the study was mycological examination of ulcerated corneal tissues from an ophthalmic patient. Tissue fragments were analyzed on potato-glucose agar (PDA) and maltose (MA) (Difco) media using standard laboratory techniques. Cultures were identified using classical and molecular methods. Macro- and microscopic colony morphology was characteristic of fungi from the genus <italic>Aspergillus</italic> (restricted growth series), most probably <italic>Aspergillus penicillioides</italic> Speg. Molecular analysis of the following rDNA regions: ITS1, ITS2, 5.8S, 28S rDNA, LSU and β-tubulin were carried out for the isolates studied. A high level of similarity was found between sequences from certain rDNA regions, i.e. ITS1-5.8S-ITS2 and LSU, what confirmed the classification of the isolates to the species <italic>A. penicillioides.</italic> The classification of our isolates to <italic>A. penicillioides</italic> species was confirmed also by the phylogenetic analysis.</p></abstract>

The aim of the study was mycological examination of ulcerated corneal tissues from an ophthalmic patient. Tissue fragments were analyzed on potato-glucose agar (PDA) and maltose (MA) (Difco) media using standard laboratory techniques. Cultures were identified using classical and molecular methods. Macro- and microscopic colony morphology was characteristic of fungi from the genus Aspergillus (restricted growth series), most probably Aspergillus penicillioides Speg. Molecular analysis of the following rDNA regions: ITS1, ITS2, 5.8S, 28S rDNA, LSU and β-tubulin were carried out for the isolates studied. A high level of similarity was found between sequences from certain rDNA regions, i.e. ITS1-5.8S-ITS2 and LSU, what confirmed the classification of the isolates to the species A. penicillioides. The classification of our isolates to A. penicillioides species was confirmed also by the phylogenetic analysis.

<italic>Aspergillus penicillioides</italic> Speg. Implicated in Keratomycosis

Polish Journal of Microbiology

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The aim of the study was mycological examination of ulcerated corneal tissues from an ophthalmic patient. Tissue fragments were analyzed on potato-glucose agar...

Aspergillus penicillioides Speg. Implicated in Keratomycosis

Article Category: original-paper

Published Online: Dec 10, 2018

Page range: 407 - 416

Received: Nov 09, 2017

Accepted: Jun 28, 2018

DOI: https://doi.org/10.21307/pjm-2018-049

Keywords
morphology, genetic characteristic, cornea

© 2018 Eulalia Machowicz-Matejko et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Aspergillus penicillioides Speg. Implicated in Keratomycosis

Article Category: original-paper

Published Online: Dec 10, 2018

Page range: 407 - 416

Received: Nov 09, 2017

Accepted: Jun 28, 2018

DOI: https://doi.org/10.21307/pjm-2018-049

Keywordsmorphology, genetic characteristic, cornea

© 2018 Eulalia Machowicz-Matejko et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
morphology, genetic characteristic, cornea