<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The pin nematode, <italic>Paratylechus beltsvillensis</italic> n. sp. collected from rhizosphere soil of a Virginia pine tree (<italic>Pinus virginiana</italic> Mill) growing in Little Paint Branch Park, Beltsville, Prince George’s County, Maryland, USA, is described and illustrated along with light and scanning electron photomicrographs. Females, males, and juveniles of this new species were recovered from soil samples using the sugar centrifugal flotation and Baermann funnel extraction methods. Morphologically, females are short, body length ranging from 245 to 267 μm, stylet from 70 to 75 μm long with anchor shaped knobs, vulva located at 70–73% and small vulval flap, spermatheca large, and ovoid filled with sperms. Lateral field with three incisures, of which the outer two are prominent. Tail slender, having a rounded tail terminus. Males without stylet and have a degenerated pharynx, spicules = 17–20 µm and gubernaculum = 5.0–5.5 µm. Both morphological observations and molecular analysis of ITS and partial 28S ribosomal RNA gene sequences indicated that the specimens collected from the soil at Beltsville Park from rhizosphere soil samples from Virginia pine represents a new pin nematode species.</p>
</abstract>

The pin nematode, Paratylechus beltsvillensis n. sp. collected from rhizosphere soil of a Virginia pine tree (Pinus virginiana Mill) growing in Little Paint Branch Park, Beltsville, Prince George’s County, Maryland, USA, is described and illustrated along with light and scanning electron photomicrographs. Females, males, and juveniles of this new species were recovered from soil samples using the sugar centrifugal flotation and Baermann funnel extraction methods. Morphologically, females are short, body length ranging from 245 to 267 μm, stylet from 70 to 75 μm long with anchor shaped knobs, vulva located at 70–73% and small vulval flap, spermatheca large, and ovoid filled with sperms. Lateral field with three incisures, of which the outer two are prominent. Tail slender, having a rounded tail terminus. Males without stylet and have a degenerated pharynx, spicules = 17–20 µm and gubernaculum = 5.0–5.5 µm. Both morphological observations and molecular analysis of ITS and partial 28S ribosomal RNA gene sequences indicated that the specimens collected from the soil at Beltsville Park from rhizosphere soil samples from Virginia pine represents a new pin nematode species.

<article-title xml:lang="en">Morphological and molecular characterization of <italic>Paratylenchus beltsvillensis</italic> n. sp. (Tylenchida: Paratylenchidae) from the rhizosphere of pine tree (<italic>Pinus virginiana</italic> Mill) in Maryland, USA

Journal of Nematology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Morphological and molecular characterization of  n. sp. (Tylenchida: Paratylenchidae) from the rhizosphere of pine tree ( Mill) in Maryland, USA

The pin nematode, Paratylechus beltsvillensis n. sp. collected from rhizosphere soil of a Virginia pine tree (Pinus virginiana Mill) growing in Little Paint...

Morphological and molecular characterization of Paratylenchus beltsvillensis n. sp. (Tylenchida: Paratylenchidae) from the rhizosphere of pine tree (Pinus virginiana Mill) in Maryland, USA

Published Online: Sep 07, 2021

Page range: 1 - 10

DOI: https://doi.org/10.21307/jofnem-2021-079

Keywords
D2-D3 of 28S rRNA gene, ITSrRNA gene, Description, Pin nematode, Morphology, Morphometrics, Phylogeny, Scanning electron microscopy, Virginia pine

© 2021 Mihail R. Kantor et al., published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Morphological and molecular characterization of Paratylenchus beltsvillensis n. sp. (Tylenchida: Paratylenchidae) from the rhizosphere of pine tree (Pinus virginiana Mill) in Maryland, USA

Published Online: Sep 07, 2021

Page range: 1 - 10

DOI: https://doi.org/10.21307/jofnem-2021-079

KeywordsD2-D3 of 28S rRNA gene, ITSrRNA gene, Description, Pin nematode, Morphology, Morphometrics, Phylogeny, Scanning electron microscopy, Virginia pine

© 2021 Mihail R. Kantor et al., published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
D2-D3 of 28S rRNA gene, ITSrRNA gene, Description, Pin nematode, Morphology, Morphometrics, Phylogeny, Scanning electron microscopy, Virginia pine