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A new aphelench nematode, Basilaphelenchus brevistylus n. sp. (Aphelenchoididae: Tylaphelenchinae) from Pinus massoniana in China

Bashiy Akol
Bashiy Akol
, 
Qiuling Huang
Qiuling Huang
, 
Borong Lin
Borong Lin
, 
Honghong Wang
Honghong Wang
, 
Jinling Liao
Jinling Liao
 and 
Kan Zhuo
Kan Zhuo
   | Aug 05, 2021
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Published Online: Aug 05, 2021
Page range: 1 - 12
DOI: https://doi.org/10.21307/jofnem-2021-070
Keywords
© 2021 Bashiy Akol et al., published by Sciendo.
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Line drawings of Basilaphelenchus brevistylus n. sp. (A) female; (B) male; (C) female anterior region; (D) vulval region; (E) female tail; (F) male tail; (G) spicule. (Scale bars: A, B = 50 µm; C-G = 10 µm).
Line drawings of Basilaphelenchus brevistylus n. sp. (A) female; (B) male; (C) female anterior region; (D) vulval region; (E) female tail; (F) male tail; (G) spicule. (Scale bars: A, B = 50 µm; C-G = 10 µm).

Figure 2:

Females of Basilaphelenchus brevistylus n. sp. under the light microscrope. (A) entire body; (B) lip region and stylet; (C) anterior region; (D) metacorpus; (E) lateral lines; (F) oocytes; (G) vulva region and post-vulval sac; (H) tail. (Scale bars: A = 100 µm; B-H = 10 µm).
Females of Basilaphelenchus brevistylus n. sp. under the light microscrope. (A) entire body; (B) lip region and stylet; (C) anterior region; (D) metacorpus; (E) lateral lines; (F) oocytes; (G) vulva region and post-vulval sac; (H) tail. (Scale bars: A = 100 µm; B-H = 10 µm).

Figure 3:

Males of Basilaphelenchus brevistylus n. sp. under the light microscrope. (A) entire body; (B) lip region and stylet; (C) testis cells; (D, F) tail and spicule; (E) tail tip; (G) papillae. (Scale bars: A = 100 µm; B-G = 10 µm).
Males of Basilaphelenchus brevistylus n. sp. under the light microscrope. (A) entire body; (B) lip region and stylet; (C) testis cells; (D, F) tail and spicule; (E) tail tip; (G) papillae. (Scale bars: A = 100 µm; B-G = 10 µm).

Figure 4:

Bayesian consensus tree inferred from D2-D3 under GTR + I + G model (‒lnL = 18,080.8008; freqA = 0.1951; freqC = 0.1764; freqG = 0.3257; freqT = 0.3028; R(a) = 0.8366; R(b) = 2.5599; R(c) = 1.221; R(d) = 0.5867; R(e) = 4.2157; R(f) = 1; Pinva = 0.1777; Shape = 0.9323). Posterior probability values exceeding 50% are given on appropriate clades.
Bayesian consensus tree inferred from D2-D3 under GTR + I + G model (‒lnL = 18,080.8008; freqA = 0.1951; freqC = 0.1764; freqG = 0.3257; freqT = 0.3028; R(a) = 0.8366; R(b) = 2.5599; R(c) = 1.221; R(d) = 0.5867; R(e) = 4.2157; R(f) = 1; Pinva = 0.1777; Shape = 0.9323). Posterior probability values exceeding 50% are given on appropriate clades.

Figure 5:

Bayesian consensus tree inferred from SSU under GTR + I + G model (‒lnL = 27,834.9805; freqA = 0.2254; freqC = 0.1985; freqG = 0.2797; freqT = 0.2964; R(a) = 1.0461; R(b) = 2.3739; R(c) = 1.4729; R(d) = 0.7716; R(e) = 2.9919; R(f) = 1; Pinva = 0.0959; Shape = 0.7961). Posterior probability values exceeding 50% are given on appropriate clades.
Bayesian consensus tree inferred from SSU under GTR + I + G model (‒lnL = 27,834.9805; freqA = 0.2254; freqC = 0.1985; freqG = 0.2797; freqT = 0.2964; R(a) = 1.0461; R(b) = 2.3739; R(c) = 1.4729; R(d) = 0.7716; R(e) = 2.9919; R(f) = 1; Pinva = 0.0959; Shape = 0.7961). Posterior probability values exceeding 50% are given on appropriate clades.

Morphometrics of Basilaphelenchus brevistylus n. sp

Female Male
Character Holotype Paratypes Paratypes
n – 15 15
L 469.1 413.6 ± 35.4 (364.3-483.1) 381.7 ± 19.6 (352.4-413.1)
a 29.7 29.2 ± 2.9 (25.2-36.8) 33.6 ± 3.0 (29.6-40.5)
b 10.4 9.9 ± 0.5 (9.4-10.9) 8.9 ± 0.5 (8-9.6)
c 18.5 17.7 ± 1.3 (16.2-20.9) 16.2 ± 1.0 (13.9-17.8)
c′ 3.0 3.1 ± 0.3 (2.7-3.5) 2.9 ± 0.2 (2.6-3.4)
V or T 72.2 71.7 ± 1.1 (69.8-73.8) 49.9 ± 4.6 (43.2-61.7)
M 30.0 32.5 ± 3.7 (28.0-38.0) 31.0 ± 4.4 (24.0-37.0)
Max. body diam. 15.8 14.1 ± 1.8 (11.4-18.0) 11.4 ± 1.1 (9.8-13.2)
Lip region diam. 4.4 4.5 ± 0.4 (4.1-5.3) 4.4 ± 0.2 (4-4.8)
Lip region height 2.5 2.4 ± 0.3 (2-2.6) 2.3 ± 0.1 (2.1-2.5)
Stylet conus 1.5 1.6 ± 0.2 (1.4-1.9) 1.4 ± 0.2 (1.1-1.8)
Stylet length (total) 5.0 4.9 ± 0.3 (4.5-5.5) 4.6 ± 0.4 (4.0-5.0)
Distal end of metacorpus from anterior 45.0 41.5 ± 2.5 (37.7-45.1) 42.6 ± 1.3 (39.0-43.7)
Metacorpus length 9.2 8.4 ± 0.6 (7.3-9.5) 8.3 ± 0.6 (7.3-9.7)
Metacorpus diam. 6.6 6.8 ± 0.7 (5.8-8.1) 6.6 ± 0.5 (5.4-7.8)
Position of the valve of metacorpus (%) 65.2 66.0 ± 3.7 (60.7-72) 67.3 ± 2.7 (63-72)
Body diam. at metacorpus level 10.9 11.0 ± 2.4 (9.7-11.7) 9.6 ± 0.7 (8.5-10.8)
Gonad length 213.3 190.1 ± 21.8 (160.1-223.9) 189.9 ± 18.4 (163.9-242.6)
Vulval body diam. 12.0 12.1 ± 1.2 (10.5-14.4) –
Spicule length (chord) – – 11.5 ± 0.7 (10.3-12.8)
Spicule length (arc) – – 10.5 ± 0.6 (9.6-11.3)
Post-vulval uterine sac length 48.8 45.9 ± 7.0 (36.6-56.5) –
Vulva-anus distance 104.9 93.7 ± 7.1 (83.1-107.3) –
Anal or cloacal body diam. 8.4 7.3 ± 0.8 (6.5-8.9) 8.0 ± 0.3 (7.5-8.7)
Tail length 25.4 22.0 ± 0.6 (20-26.6) 23.5 ± 1.6 (20.1-27.3)
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Language:
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