<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Two populations of a <italic>Butlerius</italic> species were recovered from compost in two gardens in Potchefstroom, North-West Province, South Africa. Although the genus has previously been reported from South Africa, no species of the genus has ever been identified in the country. Based on morphological, morphometric, and molecular studies, the specimens were identified as <italic>Butlerius butleri</italic> and are herein reported for the first time from South Africa. The South African specimens are 1,082 to 1,423 µm long, <italic>a</italic> = 40.8 to 47.6; <italic>b</italic> = 4.7 to 5.8; <italic>c</italic> = 4.0 to 6.0; <italic>c′</italic> = 117 to 16.3; <italic>V</italic> = 44 to 47%. Cuticle with evenly spaced punctations. Reproductive system didelphic, amphidelphic, both branches equal in length. Four large glands opening into proximal part of uterus. Males with prominent sphincter present in mid-region of <italic>vas deferens</italic>. Spicules 36 to 43 μm long, gubernaculum 23 to 31 µm long, nine pairs of genital papillae, three pre-cloacal and six post-cloacal, formula: v1, v2, v3d/v4, ad, ph, v5, 6, 7, pd. The v5, 6, 7 clusters greatly separated, left subventral group at level of phasmid, right subventral group at level of posterior dorsal papilla. Although there were some differences, the South African populations of the species compare well to all know descriptions of the species. Phylogenetic analysis based on partial small subunit (SSU) rDNA sequences showed that both South African populations of <italic>B. butleri</italic> are in a maximally supported sister relation with an Iranian population of this species. Based on large subunit (LSU) rDNA sequences, the two populations of <italic>B. butleri</italic> clustered together in a well-supported clade.</p>
</abstract>

Two populations of a Butlerius species were recovered from compost in two gardens in Potchefstroom, North-West Province, South Africa. Although the genus has previously been reported from South Africa, no species of the genus has ever been identified in the country. Based on morphological, morphometric, and molecular studies, the specimens were identified as Butlerius butleri and are herein reported for the first time from South Africa. The South African specimens are 1,082 to 1,423 µm long, a = 40.8 to 47.6; b = 4.7 to 5.8; c = 4.0 to 6.0; c′ = 117 to 16.3; V = 44 to 47%. Cuticle with evenly spaced punctations. Reproductive system didelphic, amphidelphic, both branches equal in length. Four large glands opening into proximal part of uterus. Males with prominent sphincter present in mid-region of vas deferens. Spicules 36 to 43 μm long, gubernaculum 23 to 31 µm long, nine pairs of genital papillae, three pre-cloacal and six post-cloacal, formula: v1, v2, v3d/v4, ad, ph, v5, 6, 7, pd. The v5, 6, 7 clusters greatly separated, left subventral group at level of phasmid, right subventral group at level of posterior dorsal papilla. Although there were some differences, the South African populations of the species compare well to all know descriptions of the species. Phylogenetic analysis based on partial small subunit (SSU) rDNA sequences showed that both South African populations of B. butleri are in a maximally supported sister relation with an Iranian population of this species. Based on large subunit (LSU) rDNA sequences, the two populations of B. butleri clustered together in a well-supported clade.

<article-title xml:lang="en">Morphological and molecular characterization of <italic>Butlerius butleri</italic> Goodey, 1929 (Nematoda: Diplogastridae) from South Africa: First report

Journal of Nematology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Morphological and molecular characterization of  Goodey, 1929 (Nematoda: Diplogastridae) from South Africa: First report

Two populations of a Butlerius species were recovered from compost in two gardens in Potchefstroom, North-West Province, South Africa. Although the genus has...

Morphological and molecular characterization of Butlerius butleri Goodey, 1929 (Nematoda: Diplogastridae) from South Africa: First report

Published Online: Jan 01, 2021

Page range: 1 - 12

DOI: https://doi.org/10.21307/jofnem-2021-026

Keywords
Compost, First report, Morphology, Morphometrics, Molecular biology, Systematics, Taxonomy

© 2021 Authors, published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Morphological and molecular characterization of Butlerius butleri Goodey, 1929 (Nematoda: Diplogastridae) from South Africa: First report

Published Online: Jan 01, 2021

Page range: 1 - 12

DOI: https://doi.org/10.21307/jofnem-2021-026

KeywordsCompost, First report, Morphology, Morphometrics, Molecular biology, Systematics, Taxonomy

© 2021 Authors, published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
Compost, First report, Morphology, Morphometrics, Molecular biology, Systematics, Taxonomy