The history of the species knowledge may serve as an example of confuse state of nomenclature. It started in 1914 with a first note on
The soil samples were collected in 2017 from the rhizosphere of two-year
The rapid centrifugation-flotation method (Szczygieł, 1971) was used for extracting nematodes from the soil samples. Isolated nematodes were killed and fixed, then passed through a graded series of glycerol-ethanol solutions, followed by storage in anhydrous glycerol on permanent slides (Seinhorst, 1959). In total, 47 individuals from the sample were identified to the species level morphologically via a Leica biological microscope using the keys of Andrássy (2007), Brzeski (1998), and Ghaderi et al. (2014).
In order to confirm a morphological identification, three putative
Morphometrics of
Dolyna | Grytzyv | Total | ||||
---|---|---|---|---|---|---|
Character | Females | Males | Females | Males | Females | Males |
|
20 | 10 | 15 | 2 | 35 | 17 |
|
823 ± 11 (805-840) 1.4 | 746 ± 6.0 (730-755) 0.8 | 786 ± 18 (755–805) 2.3 | 718 ± 2.8 (716-720) | 808 ± 23 (755-840) 2.8 | 742 ± 12.6 (716-755) 1.7 |
|
27.1 ± 1.1 (25.5-28.5) 4.1 | 28.1 ± 0.4 (27.6-28.6) 0.4 | 25.9 ± 0.5 (25.4-26.8) 1.9 | 28.5 ± 0.1 (28.4-28.6) | 26.6 ± 1.0 (25.4-28.5) 4.0 | 28.2 ± 0.4 (27.6-28.6) 1.4 |
|
5.4 ± 0.1 (5.3-5.4) 1.4 | 4.6 ± 0.1 (4.4-4.8) 2.8 | 4.7 ± 0.2 (4.5-5.0) 4.2 | 4.9 ± 0.1 (4.8-4.9) | 5.1 ± 0.3 (4.5-5.4) 7.2 | 4.7 ± 0.1 (4.4-4.9) 3.1 |
|
14.2 ± 0.8 (12.8-15.4) 5.9 | 12.7 ± 0.3 (12.2-13.2) 2.5 | 15.5 ± 0.7 (14.2-16.6.) 5.2 | 12.7 ± 0.2 (12.5-12.8) | 14.8 ± 1.0 (12.8-16.6) 6.7 | 12.7 ± 0.3 (12.2-13.2) 2.3 |
|
3.0 ± 0.2 (2.6-3.4) 9.2 | 3.4 ± 0.1 (3.3-3.5) 9.2 | 2.7 ± 0.2 (2.7-2.9) 8.0 | 3.4 ± 0.1 (3.3-3.4) | 2.9 ± 0.2 (2.6-3.4) 10.0 | 3.4 ± 0.1 (3.3-3.5) 2.6 |
|
54.5 ± 7.6 (50-59) 13.9 | – | 53.7 ± 1.2 (52-56) 2.8 | – | 54.1 ± 5.7 (50-59) 10.6 | – |
Stylet length | 26.8 ± 1.4 (25-28) 5.2 | 26.2 ± 1.2 (25-28) 4.6 | 26.8 ± 0.8 (25-29) 3.2 | 26.5 ± 0.7 (26-27) | 26.8 ± 1.2 (25-29) 3.2 | 26.3 ± 1.1 (25-28) 4.3 |
Excretory pore | 115.8 ± 3.4 (110-120) 2.9 | 112.2 ± 1.8 (110-115) 1.6 | 121.2 ± 3.6 (116-124) 2.9 | 121 ± 1.4 (120-122) | 118.1 ± 4.4 (110-124) 3.7 | 113.7 ± 3.8 (110-122) 3.4 |
Hemizonid length | 3.5 ± 0.1 (3.5-3.6) 1.4 | – | 3.6 ± 0.1 (3.5-3.8) 2.7 | – | 3.6 ± 0.1 (3.5-3.8) 2.7 | – |
Body width | 33.4 ± 1.2 (32-36) 3.5 | – | 31.4 ± 1.2 (30-34) 3.9 | – | 32.5 ± 1.6 (30-36) 4.9 | – |
Tail annuli | 52.6 ± 1.4 (50-54) 2.7 | – | 56.9 ± 3.9 (48-62) 7 | – | 54.4 ± 3.5 (48-62) 6.4 | – |
Tail length | 58.5 ± 5.4 (49-68) 9.2 | – | 56.4 ± 1.7 (54-58) 3.0 | – | 57.6 ± 4.3 (49-68) 3.6 | – |
Phasmids on tail /% | 46.0 ± 1.9 (42-48) 4.2 | – | 45.2 ± 1.1 (44-47) 2.7 | – | 45.6 ± 1.6 (42-48) 3.7 | – |
Hyaline/tail % | 19.6 ± 1.3 (18-22) 7.0 | – | 20.6 ± 1.1 (18-22) 5.7 | – | 20.0 ± 1.3 (18-22) 6.8 | – |
Gubernaculum | – | 12.1 ± 1.3 (10-14) 11.3 | – | 11 ± 1.4 (10-12) | – | 11.9 ± 1.4 (10-14) 11.6 |
Spicule length | – | 30.7 ± 2.4 (28-34) 7.8 | – | 32.5 ± 0.4 (32-33) | – | 31 ± 2.3 (28-34) 7.4 |
Female body C-shaped:
Male body C-shaped:
The amplification of the almost full-length 18S rDNA fragment was successful (1,670 bp, MK952146). The Basic Local Alignment Search Tool (Blast) showed a 99.52% similarity to the sequence of
To the best of our knowledge, this is the first record of