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Description of Deladenus brevis n. sp. (Sphaerularioidea: Neotylenchidae) from Iran: a morphological and molecular phylogenetic study

Fariba Heydari

Fariba Heydari

Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares UniversityTehran, Iran
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Heydari, Fariba
, 
Joaquín Abolafia

Joaquín Abolafia

Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus Las LagunillasJaén, Spain
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Abolafia, Joaquín
 and 
Majid Pedram

Majid Pedram

Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares UniversityTehran, Iran
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Pedram, Majid
  
Jul 28, 2020
Description of Deladenus brevis n. sp. (Sphaerularioidea: Neotylenchidae) from Iran: a morphological and molecular phylogenetic study's Cover Image
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Published Online: Jul 28, 2020
Page range: 1 - 13
Received: Mar 26, 2020
DOI: https://doi.org/10.21307/jofnem-2020-062
Keywords
© 2020 Fariba Heydari et al., published by Sciendo.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Line drawings of Deladenus brevis n. sp. (A, B) Pharynx of male and female; (C) Anterior end of female; (D) Female genital tract and posterior body region; (E) Male posterior body region; (F) Lateral lines; (G) Female tail.
Line drawings of Deladenus brevis n. sp. (A, B) Pharynx of male and female; (C) Anterior end of female; (D) Female genital tract and posterior body region; (E) Male posterior body region; (F) Lateral lines; (G) Female tail.

Figure 2:

Photomicrographs of Deladenus brevis n. sp. (A, B) Anterior body region of female (fresh sample in water and permanently preserved specimen in glycerin respectively); (C) Part of pharynx (arrowhead showing excretory pore and C1 showing details of stylet); (D) Part of female reproductive system; (E) Female posterior body region; (F1 and F2) Lateral vulval flaps in ventrolateral and lateral view in two specimens; (G) Lateral lines; (H) Tip of ovary (arrowhead); (I, J) Male posterior body (Scale bars: A-F2, H-J =10 µm, F1, C1, G =5 µm).
Photomicrographs of Deladenus brevis n. sp. (A, B) Anterior body region of female (fresh sample in water and permanently preserved specimen in glycerin respectively); (C) Part of pharynx (arrowhead showing excretory pore and C1 showing details of stylet); (D) Part of female reproductive system; (E) Female posterior body region; (F1 and F2) Lateral vulval flaps in ventrolateral and lateral view in two specimens; (G) Lateral lines; (H) Tip of ovary (arrowhead); (I, J) Male posterior body (Scale bars: A-F2, H-J =10 µm, F1, C1, G =5 µm).

Figure 4:

Bayesian 50% majority rule consensus tree of Deladenus brevis n. sp. based on SSU rDNA sequences under GTR + I + G model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.
Bayesian 50% majority rule consensus tree of Deladenus brevis n. sp. based on SSU rDNA sequences under GTR + I + G model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.

Figure 3:

Deladenus brevis n. sp. (Scanning Electron Microscopy [SEM]). (A-C) Anterior end in ventral, sublateral and frontal views, respectively. (D) Deirid (white arrow) and excretory pore in lateral view (black arrow). (E) Excretory pore in ventral view (arrow). (F) Lateral field. (G, H) Vulva in lateral and ventral views, respectively. (I) Anus in ventral view (arrow). (J, K) Female posterior end in lateral and ventral views, respectively (arrow pointing the anus). (L, M) Male posterior end in lateral and subventral views, respectively.
Deladenus brevis n. sp. (Scanning Electron Microscopy [SEM]). (A-C) Anterior end in ventral, sublateral and frontal views, respectively. (D) Deirid (white arrow) and excretory pore in lateral view (black arrow). (E) Excretory pore in ventral view (arrow). (F) Lateral field. (G, H) Vulva in lateral and ventral views, respectively. (I) Anus in ventral view (arrow). (J, K) Female posterior end in lateral and ventral views, respectively (arrow pointing the anus). (L, M) Male posterior end in lateral and subventral views, respectively.

Figure 5:

Bayesian 50% majority rule consensus tree of Deladenus brevis n. sp. based on LSU rDNA D2 to D3 sequences under GTR + I + G model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.
Bayesian 50% majority rule consensus tree of Deladenus brevis n. sp. based on LSU rDNA D2 to D3 sequences under GTR + I + G model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.

Figure 6.

Bayesian 50% majority rule consensus tree of Deladenus brevis n. sp. based on COI mtDNA sequences under GTR + I + G model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.
Bayesian 50% majority rule consensus tree of Deladenus brevis n. sp. based on COI mtDNA sequences under GTR + I + G model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.

Morphometrics of Deladenus brevis n_ sp_

Female Male
Character

Holotype

Paratypes

Paratypes
n − 8

2
L 435

415.5 ± 27.0 (367-454)

391, 350

a

26.0 28 ± 3 (22.3-32.3)

35.5, 29.1

b

9.5 8.9 ± 0.7 (8.0-10.3)

8.0, 7.6

c

15.0

16.8 ± 2.0 (14.3-20.0)

17.7, 15.2

c′

3.2 2.8 ± 0.3 (2.4-3.2)

2.7, 2.9
T or V 88.9 89.0 ± 1.0 (87.2-90.3)

82.3, 85.3

m

39.5

39.0 ± 3.0 (33.3-43.1)

50.9, 46.3

Cephalic region height

 		1.4 1.9 ± 0.2 (1.5-2.1)

2.0, 1.5

Cephalic region width

 		5.7 5.9 ± 0.4 (5.0-6.5)

5.2, 6

Max. body dim.

 		17 15 ± 2 (13-18)

11, 13

Stylet total length

 		6.6 6.4 ± 0.3 (6-7)

6.0, 6.5

Stylet conus

 		2.6 2.5 ± 0.2 (2-3)

2.2, 2.5

Excretory pore from anterior

 		78 79.0 ± 5.3 (67-86)

75.0, 70.5

Nerve ring from anterior

 		71

71.5 ± 9.0 (60-83)

52, ?

Hemizonid from anterior

 		76 77.5 ± 6.0 (66-86)

72, 69

Vulval body diameter

 		13 12.5 ± 2. (10-18)

−

Anal body diameter

 		9 9.0 ± 1.2 (7-10)

8, 9

Tail

 		29 25.0 ± 3.4 (20-30)

22, 23

Spicules

 		− −

11.3, 14.5

Gubernaculum

 		− −

3.4, 4.5
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