<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The 18S small subunit (SSU) ribosomal DNA sequence is one of the most useful molecular loci for identification and phylogeny reconstruction of agriculturally important nematodes. Various pairs of universal primers have been developed in the past to amplify short and long nematode sequences. However, certain nematode taxa were not readily amplified and/or sequenced with the existing primer tools. Frequently, the center region of a roughly 1,000 nucleotide segment would be lost. Therefore new primers were developed based on a very large 276 taxon alignment of 124 agriculturally important nematode species, and tested on problematic nematode taxa such as <italic>Aphelenchoides, Bursaphelenchus, Ditylenchus</italic>, and <italic>Panagrolaimus</italic>. New primers and protocols are provided for successful generation of sequences useful in future investigations of nematode systematics.</p>
</abstract>

The 18S small subunit (SSU) ribosomal DNA sequence is one of the most useful molecular loci for identification and phylogeny reconstruction of agriculturally important nematodes. Various pairs of universal primers have been developed in the past to amplify short and long nematode sequences. However, certain nematode taxa were not readily amplified and/or sequenced with the existing primer tools. Frequently, the center region of a roughly 1,000 nucleotide segment would be lost. Therefore new primers were developed based on a very large 276 taxon alignment of 124 agriculturally important nematode species, and tested on problematic nematode taxa such as Aphelenchoides, Bursaphelenchus, Ditylenchus, and Panagrolaimus. New primers and protocols are provided for successful generation of sequences useful in future investigations of nematode systematics.

Improved 18S small subunit rDNA primers for problematic nematode amplification

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

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The 18S small subunit (SSU) ribosomal DNA sequence is one of the most useful molecular loci for identification and phylogeny reconstruction of agriculturally...

Improved 18S small subunit rDNA primers for problematic nematode amplification

Published Online: Dec 03, 2018

Page range: 533 - 542

DOI: https://doi.org/10.21307/jofnem-2018-051

Keywords
Barcode, Molecular sequence, Nematode identification

© 2018 L. K. Carta et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Improved 18S small subunit rDNA primers for problematic nematode amplification

Published Online: Dec 03, 2018

Page range: 533 - 542

DOI: https://doi.org/10.21307/jofnem-2018-051

KeywordsBarcode, Molecular sequence, Nematode identification

© 2018 L. K. Carta et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
Barcode, Molecular sequence, Nematode identification