<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Two different nematode species were recovered from pomegranate decaying fruit in two localities in Southern Italy: the mycetophagus nematode <italic>Sheraphelenchus sucus</italic> and a bacterial feeder nematode belonging to the Panagrolaimidae (Rhabditida) family. Morphometrics of the Italian population of <italic>S. sucus</italic> closely resemble that of the type population, whereas some differences were found when compared with another population from Iran. Molecular characterization of the Italian <italic>S. sucus</italic> using the 18S rRNA gene, D2–D3 expansion domains of the 28S rDNA, the ITS region, and the partial mitochondrial COI were carried out. Sequences of the 18S rRNA gene, the D2–D3 domains, and the ITS were analyzed using several methods for inferring phylogeny to reconstruct the relationships among <italic>Sheraphelenchus</italic> and <italic>Bursaphelenchus</italic> species. The bacterial feeder <italic>Panagrellus</italic> sp. was characterized at the molecular level only. The D2–D3 expansion domains and ITS sequences of this Italian panagrolaimid were determined. The D2–D3 sequences of the Italian panagrolaimid showed 99% similarity with the corresponding sequence of <italic>Panagrellus</italic> sp. associated with <italic>Rhynchophorus ferrugineus</italic>. This is the first report on the tritrophic association of <italic>S. sucus</italic> and Rhabditida that uses both insects and pomegranate fruit as hosts.</p>
</abstract>

Two different nematode species were recovered from pomegranate decaying fruit in two localities in Southern Italy: the mycetophagus nematode Sheraphelenchus sucus and a bacterial feeder nematode belonging to the Panagrolaimidae (Rhabditida) family. Morphometrics of the Italian population of S. sucus closely resemble that of the type population, whereas some differences were found when compared with another population from Iran. Molecular characterization of the Italian S. sucus using the 18S rRNA gene, D2–D3 expansion domains of the 28S rDNA, the ITS region, and the partial mitochondrial COI were carried out. Sequences of the 18S rRNA gene, the D2–D3 domains, and the ITS were analyzed using several methods for inferring phylogeny to reconstruct the relationships among Sheraphelenchus and Bursaphelenchus species. The bacterial feeder Panagrellus sp. was characterized at the molecular level only. The D2–D3 expansion domains and ITS sequences of this Italian panagrolaimid were determined. The D2–D3 sequences of the Italian panagrolaimid showed 99% similarity with the corresponding sequence of Panagrellus sp. associated with Rhynchophorus ferrugineus. This is the first report on the tritrophic association of S. sucus and Rhabditida that uses both insects and pomegranate fruit as hosts.

Occurrence of <italic>Sheraphelenchus sucus</italic> (Nematoda: Aphelenchoidinae) and <italic>Panagrellus</italic> sp. (Rhabditida: Panagrolaimidae) Associated with Decaying Pomegranate Fruit in Italy

Journal of Nematology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Occurrence of  (Nematoda: Aphelenchoidinae) and  sp. (Rhabditida: Panagrolaimidae) Associated with Decaying Pomegranate Fruit in Italy

Two different nematode species were recovered from pomegranate decaying fruit in two localities in Southern Italy: the mycetophagus nematode Sheraphelenchus...

Occurrence of Sheraphelenchus sucus (Nematoda: Aphelenchoidinae) and Panagrellus sp. (Rhabditida: Panagrolaimidae) Associated with Decaying Pomegranate Fruit in Italy

Published Online: Sep 26, 2018

Page range: 418 - 426

Received: Apr 04, 2017

DOI: https://doi.org/10.21307/jofnem-2017-091

Keywords
18S rRNA gene, D2–D3 expansion domains, molecular analysis, Panagrolaimidae, phylogenesis,

© 2017 ELENA FANELLI et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Occurrence of Sheraphelenchus sucus (Nematoda: Aphelenchoidinae) and Panagrellus sp. (Rhabditida: Panagrolaimidae) Associated with Decaying Pomegranate Fruit in Italy

Published Online: Sep 26, 2018

Page range: 418 - 426

Received: Apr 04, 2017

DOI: https://doi.org/10.21307/jofnem-2017-091

Keywords18S rRNA gene, D2–D3 expansion domains, molecular analysis, Panagrolaimidae, phylogenesis,

© 2017 ELENA FANELLI et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
18S rRNA gene, D2–D3 expansion domains, molecular analysis, Panagrolaimidae, phylogenesis,