Characterization of human anti-hr^B-like monoclonal antibody

To develop a blood typing reagent with Rh specificity, peripheral blood lymphocytes were isolated from a multiparous woman with apparent alloanti-Ce and fused with FIS heteromyeloma cells. One hybridoma cell line, FOR-2E3, secreted human IgG, which agglutinated all human red blood cells except R₂R₂, R_zR_z, Dc–, D––, Rh_null, and e+ hr^B–. The supernatant is useful as a reagent to screen for hr^B– blood donors and to differentiate haplotypes with C and e in cis from haplotypes with C and e in trans.