<abstract xmlns="http://www.w3.org/1999/xhtml">To develop a blood typing reagent with Rh specificity, peripheral blood lymphocytes were isolated from a multiparous woman with apparent alloanti-Ce and fused with FIS heteromyeloma cells. One hybridoma cell line, FOR-2E3, secreted human IgG, which agglutinated all human red blood cells except R2R2, RzRz, Dc–, D––, Rhnull, and e+ hrB–. The supernatant is useful as a reagent to screen for hrB– blood donors and to differentiate haplotypes with <italic>C</italic> and <italic>e</italic> in <italic>cis</italic> from haplotypes with <italic>C</italic> and <italic>e</italic> in <italic>trans</italic>.</abstract>

To develop a blood typing reagent with Rh specificity, peripheral blood lymphocytes were isolated from a multiparous woman with apparent alloanti-Ce and fused with FIS heteromyeloma cells. One hybridoma cell line, FOR-2E3, secreted human IgG, which agglutinated all human red blood cells except R2R2, RzRz, Dc–, D––, Rhnull, and e+ hrB–. The supernatant is useful as a reagent to screen for hrB– blood donors and to differentiate haplotypes with C and e in cis from haplotypes with C and e in trans.

Characterization of human anti-hrB-like monoclonal antibody

Immunohematology

Characterization of human anti-hr-like monoclonal antibody

To develop a blood typing reagent with Rh specificity, peripheral blood lymphocytes were isolated from a multiparous woman with apparent alloanti-Ce and fused...

Characterization of human anti-hr^B-like monoclonal antibody

Published Online: Nov 16, 2020

Page range: 119 - 122

DOI: https://doi.org/10.21307/immunohematology-2019-762

© 1996 A.P. Blancher et al., published by Sciendo