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Sulfhydryl treatment of serum or plasma for the reduction of IgM antibodies
Oct 16, 2019
About this article
Published Online: Oct 16, 2019
Page range: 135 - 139
DOI: https://doi.org/10.21307/immunohematology-2018-021
Keywords
© 2018 L.N. Blagg, published by Sciendo
Dithiothreitol (DTT) and 2-mercaptoethanol (2-ME) are sulfhydryl compounds that can be used to treat serum or plasma to denature IgM antibody reactivity. By using sulfhydryl agents, IgG and IgM antibodies can be separated, the relative amount of IgM and IgG antibodies can be determined, and the risk of hemolytic disease of the fetus and newborn can be assessed.