1. bookVolume 59 (2010): Issue 1-6 (December 2010)
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
access type Open Access

Use of DNA-Fingerprints to Control the Origin of Forest Reproductive Material

Published Online: 20 Oct 2017
Volume & Issue: Volume 59 (2010) - Issue 1-6 (December 2010)
Page range: 268 - 273
Received: 28 May 2010
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Abstract

Well-adapted, high quality reproductive material is key to the success of forest plantations. Consequently in many countries the collection and trade of forest reproductive material is regulated. Paper documents are usually the only evidence for the origin of forest reproductive material. Certification schemes already established in Germany use genetic inventories to compare reference samples collected at different steps of the chain-ofcustody. A new approach using DNA-fingerprints efficiently controls the origin of seed sources without these multiple reference samples. Only a sample of adult trees within the seed stands is needed. The control is directly made for each suspicious plant or a group of suspicious plants by use of multilocus genotype assignment. We made a field test with samples of adults and seedling from 5 registered seed stands of Quercus robur in Western Germany. Eight highly variable nuclear microsatellites were used to genotype each individual. We found in total 255 different alleles at all loci in the adult populations. The observed levels of genetic variation (Ae= 9.18), genetic differentiation (delta = 0.187) and population fixation (FST = 0.01) were slightly higher than results of similar studies. Individual and group assignment tests were performed with the Bayesian multi-locus approach. The proportion of correctly assigned seedlings was 65% for individuals with completely scored genotypes. In all 5 cases the groups of seedlings were assigned to the correct seed stand and an additional sample of seedlings from another stand could be successfully excluded with a probability test. The conclusion of the field study is that a large scale application of this new approach to control of the origin of forest reproductive material is feasible.

Keywords

BEHM, A. and M. KONNERT (2000): Proposal for a seed certification scheme. Dendrobiology 47, 105-108.Search in Google Scholar

COLPAERT, N., S. CAVERS, E. BANDOU, H. CARON, G. GHEYSEN and A. J. LOWE (2005): Sampling Tissue for DNA Analysis of Trees: Trunk Cambium as an Alternative to Canopy leaves. Silvae Genet. 54, 265-269.10.1515/sg-2005-0038Search in Google Scholar

CORNUET, J. M., S. PIRY, G. LUIKART, A. ESTOUP and M. SOLIGNAC (1999): New methods employing multilocus genotypes to select or exclude populations as origins of individuals. Genetics 153: 1989-2000.10.1093/genetics/153.4.1989Search in Google Scholar

DUMOLIN, S., B. DEMESURE and R. J. PETIT (1995): Inheritance of chloroplast and mitochondrial genomes in pediculate oak investigated with an efficient PCR method. Theor. Appl. Genetics 91, 1253-1256.Search in Google Scholar

GREGORIUS, H.-R., B. DEGEN and A. KOENIG (2007): Problems in the analysis of genetic differentiation among populations - A case study in Quercus robur. Silvae Genet. 56, 190-199.10.1515/sg-2007-0029Search in Google Scholar

GREGORIUS, H.-R. (1978): The concept of genetic diversity and its formal relationship to heterozygosity and genetic distance. Math. Bioscience 41, 253-271.10.1016/0025-5564(78)90040-8Open DOISearch in Google Scholar

HEDRICK, P. W. (2005): A standardized genetic differentiation measure. Evolution 59, 1633-1638.10.1111/j.0014-3820.2005.tb01814.xSearch in Google Scholar

HONJO, M., S. Ueno, Y. TSUMURA, T. HANDA, I. Washitani and R. OHSAWA (2008): Tracing the origins of stocks of the endangered species Primula sieboldii using nuclear microsatellites and chloroplast DNA. Conservation Genetics 9, 1139-1147.10.1007/s10592-007-9427-7Open DOISearch in Google Scholar

KAMPFER, S., C. LEXER, J. GLÖSSL and H. STEINKELLNER (1998): Characterization of (GA)n microsatellite loci from Quercus robur. Hereditas 129, 183-186.10.1111/j.1601-5223.1998.00183.xSearch in Google Scholar

KOENIG, A. O., B. ZIEGENHAGEN, B. C.V. DAM, U. M. CSAIKL, E. COART, B. DEGEN, K. BURG, S. M. G. D. VRIES and R. J. PETIT (2002): Chloroplast DNA variation of oaks in western Central Europe and genetic consequences of human influences. For. Ecol. Manage. 156, 147-166.Search in Google Scholar

KONNERT, M. and W. RUETZ (2006): Genetic aspects of artificial regeneration of Douglas-fir (Pseudotsuga menziesii) in Bavaria. Eur. J. For. Res. 125, 261-270.Search in Google Scholar

KONNERT, M. (2006): Success with origin evidence using isoenzym and DNA analyses. AFZ/Wald, Allg. Forst Z. Waldwirtsch. Umweltvorsorge 61.Search in Google Scholar

LEPAIS, O., V. LÉGER and S. GERBER (2006): Short Note: High Throughput Microsatellite Genotyping in Oak Species. Silvae Genet. 55, 238-240.10.1515/sg-2006-0031Search in Google Scholar

LEPAIS, O., J. PETIT, E. GUICHOUX, J. E. LAVABRE, F. ALBERTO, A. KREMER and S. GERBER (2009): Species relative abundance and direction of introgression in oaks. Mol. Ecol. 18, 2228-2242.10.1111/j.1365-294X.2009.04137.x19302359Open DOISearch in Google Scholar

MANLY, B. F. J. (1991): Randomization and Monte Carlo Methods in Biology. Chapman and Hall, London.10.1007/978-1-4899-2995-2Search in Google Scholar

MARSHALL, T. C., J. SLATE, L. E. B. KRUUK and J. M. PEMBERTON (1998): Statistical confidence for likelihoodbased paternity inference in natural populations. Molecular Ecology 7: 639-655.10.1046/j.1365-294x.1998.00374.x9633105Open DOISearch in Google Scholar

MUIR, G., A. J. LOWE, C. C. FLEMING and C. VOGL (2004): High nuclear genetic diversity, high levels of outcrossing and low differentiation among remnant populations of Quercus petraea at the margin of its range in Ireland. Ann. Bot. 93, 691-697.10.1093/aob/mch096424230115087303Search in Google Scholar

NARUM, S. R., M. BANKS, T. D. BEACHAM, M. R. BELLINGER, M. R. CAMPBELL, J. DEKONING, A. ELZ, C. M. GUTHRIE, C. KOZFKAY, K. M. MILLER, P. MORAN, R. PHILLIPS, L.W. SEEB, C. T. SMITH, K. WARHEIT, S. F. YOUNG and J. C. GARZA (2008): Differentiating salmon populations at broad and fine geographical scales with microsatellites and single nucleotide polymorphisms. Mol. Ecol. 17, 3464-3477.Search in Google Scholar

OGDEN, R. (2008): Fisheries forensics: the use of DNA tools for improving compliance, traceability and enforcement in the fishing industry. Fish and Fisheries 9, 462-472. 10.1111/j.1467-2979.2008.00305.xOpen DOISearch in Google Scholar

ORRU, L., G. CATILLO, F. NAPOLITANO, G. DE MATTEIS, M. C. SCATA, F. SIGNORELLI and B. MOIOLI (2009): Characterization of a SNPs panel for meat traceability in six cattle breeds. Food Control 20, 856-860.10.1016/j.foodcont.2008.10.015Open DOISearch in Google Scholar

PIRY, S., A. ALAPETITE, J. M. CORNUET, D. PAETKAU, L. BAUDOUIN and A. ESTOUP (2004): GENECLASS2: A software for genetic assignment and first-generation migrant detection. J. Hered. 95, 536-539.10.1093/jhered/esh07415475402Search in Google Scholar

RANNALA, B. and J. L. MOUNTAIN (1997): Detecting immigration by using multilocus genotypes. Proc. Natl. Acad. Sci. U.S.A. 94, 9197-9201.10.1073/pnas.94.17.9197231119256459Open DOISearch in Google Scholar

STEINKELLNER, H., S. FLUCH, E. TURETSCHEK, C. LEXER, R. STREIFF, A. KREMER, K. BURG and J. GLÖSSL (1997): Identification and characterization of (GA/CT)n microsatellite loci from Quercus petraea. Plant Mol. Biol. 33, 1093-1096.10.1023/A:1005736722794Open DOISearch in Google Scholar

WASSER, S. K., C. MAILAND, R. BOOTH, B. MUTAYOBA, E. KISAMO, B. CLARK and M. STEPHENS (2007): Using DNA to track the origin of the largest ivory seizure since the 1989 trade ban. Proc. Natl. Acad. Sci. U.S.A. 104, 4228-4233.Search in Google Scholar

WASSER, S. K., A. M. SHEDLOCK, K. COMSTOCK, E. A. OSTRANDER, B. MUTAYOBA and M. STEPHENS (2004): Assigning African elephant DNA to geographic region of origin: Applications to the ivory trade. Proc. Natl. Acad. Sci. U.S.A. 101, 14847-14852.Search in Google Scholar

WEIR, B. S. (1990): Genetic Data Analysis. Sinauer Associates, Sunderland, MA. Search in Google Scholar

XING, J. C., W. S. WATKINS, D. J. WITHERSPOON, Y. H. ZHANG, S. L. GUTHERY, R. THARA, B. J. MOWRY, K. BULAYEVA, R. B. WEISS and L. B. JORDE (2009): Finescaled human genetic structure revealed by SNP microarrays. Genome Research 19, 815-825.10.1101/gr.085589.108267597019411602Open DOISearch in Google Scholar

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