1. bookVolume 56 (2007): Issue 1-6 (December 2007)
Journal Details
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Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
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1 time per year
Languages
English
Open Access

Cross-Species Amplification of Coconut Microsatellite Markers in Rattans

Published Online: 14 Oct 2017
Volume & Issue: Volume 56 (2007) - Issue 1-6 (December 2007)
Page range: 282 - 286
Received: 22 Aug 2006
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Summary

Cross-species amplification of microsatellite loci is considered to be a time-saving as well as a cost-effective approach for developing locus specific markers for new species. In an attempt to identify molecular markers to determine gene flow events and genetic differentiation of populations in rattans, microsatellite primer pairs developed for Cocos nucifera (Arecaceae) were examined for cross-species amplification in rattans. Of the six microsatellite primer pairs screened, two (CNZ34 and CN2A5) yielded good cross-species amplification across different rattan genera and species from South and Southeast Asia. Based on microsatellite-specific PCR amplification products, cluster analysis was performed. The dendrogram indicated a clear grouping of rattans based on their genera and species. The coconut locus specific primer pairs could be successfully used to construct molecular differentiation of rattan genera across different geographical locations.

Keywords

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