1. bookVolume 56 (2007): Issue 1-6 (December 2007)
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
access type Open Access

Identification of AFLP Markers Associated with Embryonic Root Development in Populus tomentosa Carr

Published Online: 14 Oct 2017
Volume & Issue: Volume 56 (2007) - Issue 1-6 (December 2007)
Page range: 27 - 32
Received: 22 Nov 2005
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Abstract

Embryonic root (radicle) development in the mature embryo following germination is essential for the formation of the root organ in plants. In this study a phenotype described by a lack of proper radicle development was identified in an intraspecific hybrid of Populus tomentosa Carr.. Association of this trait with Amplified Fragment Length Polymorphisms (AFLPs) markers was investigated in a segregating F1 population generated by intraspecific-controlled crossing between a highly fertile female P. tomentosa clone “5082” and a male P. tomentosa clone “JY”. A total of 3193 seeds were obtained, and the rate of germination found to be 48.74% at 15 to 20 days. 376 (24%) of seedlings were shown to lack a root organ following visual assessment of the developing radicle. Genetic regulation of this trait appeared to be via a single dominant gene or a set of tightly linked genes, based on the 3:1 ratio of the rooting versus nonrooting seed embryos. A Bulked Segregant Analysis approach using 5600 AFLP markers was applied to this population and revealed 2 AFLP markers, EcoRI + GAG/ Mse I + AAT-492 and EcoRI + GAG/Mse I + CCA-502, that were associated with the radicle development-controlling locus in P. tomentosa. The AFLP markers identified have potential for application in hybrid breeding via marker assisted selection, and provide a starting point for map based cloning of the radical development-controlling gene.

Keywords

BENFEY, P. N., P. J. LINSTEAD, K. ROBERTS, J. W. SCHIEFELBEIN, M. T. HAUSER and R. A. AESCHBACHER (1993): Root development in Arabidopsis: four mutants with dramatically altered root morphogenesis. Development 119: 57-70.10.1242/dev.119.1.57Search in Google Scholar

CELENZA, J. L. JR, P. L. GRISAFI and G. R. FINK (1995): A pathway for lateral root formation in Arabidopsis thaliana. Genes Dev 9: 2131-2142.10.1101/gad.9.17.21317657165Search in Google Scholar

CERVERA, M. T., J. GUSMAO, M. STEENACKERS, J. PELEMAN, V. STORME, A. VAN DEN BROECK, M. VAN MONTAGU and W. BOERJAN (1996): Identification of AFLP molecular markers for resistance against Me-lampsora larici-populina in Populus. Theor Appl Genet 93: 733-737.10.1007/BF0022406924162401Open DOISearch in Google Scholar

CHENG, J. C., K. A. SEELEY and Z. R. SUNG (1995): RML1 and RML2, Arabidopsis genes required for cell proliferation at the root tip. Plant Physiol 107: 365-376.10.1104/pp.107.2.3651571367724670Search in Google Scholar

HOISINGTON, D., M. KHAIRALLAH and D. GONZALES-DE-LEON (1994): Laboratory protocols CIMMYT. 2nd edn. Applied molecular genetics laboratory. CIMMYT. EI Batan, Mexico DF.Search in Google Scholar

KONDO, T., K. TERADA, E. HAYASHI, N. KURAMOTO, M. OKAMURA and H. KAWASAKI (2000): RAPD markers linked to a gene for resistance to pine needle gall midge in Japanese black pine (Pinus thunbergii). Theor Appl Genet 100: 391-395.10.1007/s001220050051Open DOISearch in Google Scholar

KOSAMBI, D. D. (1994): The estimation of map distances from recombination values. Ann Eugen 12: 172-175.10.1111/j.1469-1809.1943.tb02321.xSearch in Google Scholar

KUBO, H., A. J. M. PEETERS, M. G. M. AARTS, A. PEREIRA and M. KOORNNEEF (1999): ANTHOCYANINLESS2, a homeobox gene affecting anthocyanin distribution and root development in Arabidopsis. Plant Cell 11: 1217-1226.10.1105/tpc.11.7.121714428310402424Open DOISearch in Google Scholar

LAUX, T. and G. JÜRGENS (1997): Embryogenesis: a new start in life. Plant Cell 9: 989-1000.10.1105/tpc.9.7.98915697312237371Open DOISearch in Google Scholar

MATHER, K. (1938): The measurement of linkage in heredity (Methuen’s monographs on biological subjects). Methuen, London.Search in Google Scholar

MAYER, U., R. A. TORRES RUIZ, T. BERLETH, S. MISERA and G. JÜRGENS (1991): Mutations affecting body organization in the Arabidopsis embryo. Nature 353: 402-407.10.1038/353402a0Search in Google Scholar

MICHELMORE, R. W., I. PARAN and R. V. KESSELI (1991): Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88: 9828-9832.10.1073/pnas.88.21.9828528141682921Open DOISearch in Google Scholar

NATESH, S. and M. A. RAU (1984): The embryo. In: embryology of angiosperms. (ed. B. M. JOHRI), Springer-Verlag.10.1007/978-3-642-69302-1_8Search in Google Scholar

SAX, K. (1923): The association of size differences with seed-coat pattern and pigmentation in Phaseolus vulgaris. Genetics 8: 552-560.1724602610.1093/genetics/8.6.552120076517246026Search in Google Scholar

SCHERES, B., H. WOLKENFELT, V. WILLEMSEN, M. TERLOUW, E. LAWSON, C. DEAN and P. WEISBEEK (1994): Embryonic origin of the Arabidopsis primary root and root meristem initials. Development 120: 2475-2487.10.1242/dev.120.9.2475Search in Google Scholar

STIRLING, B., G. NEWCOMBE, J. VREBALOV, I. BOSDET and H. D. BRADSHAW (2001) Suppressed recombination around the MXC3 locus, a major gene for resistance to poplar leaf rust. Theor Appl Genet 103: 1129-1137.10.1007/s001220100721Search in Google Scholar

TIXIER, M. H., R. M. SOURDILLE, P. LEROY and M. BERNARD (1997): Detection of wheat microsatellites using a non radioactive silver-nitrate staining method. J Genet Breed 51: 175-177.Search in Google Scholar

VERNOUX, T., R. C. WILSON, K. A. SEELEY, J. P. REICHHELDT, S. MUROY, S. BROWN, S. C. MAUGHAN, C. S. COBBETT, M. V. MONTAGU, D. INZÉ, M. J. MAY and Z. R. SUNG (2000): The ROOT MERISTEMLESS1/ CADMIUM SENSITIVE2 gene defines a glutathionedependent pathway involved in initiation and maintenance of cell division during postembryonic root development. Plant Cell 12: 97-110.10.1105/tpc.12.1.9714021710634910Open DOISearch in Google Scholar

VILLAR, M., F. LEFÈVRE, H. D. BRADSHAW and E. T. DUCROS (1996): Molecular genetics of rust resistance in poplars (Melampsora larici-populina Kleb/Populus sp.) by bulked segregant analysis in a 2 x 2 factorial mating design. Genetics 143: 531-536.10.1093/genetics/143.1.53112072848722801Search in Google Scholar

VOS, P., R. HOGERS, M. BLEEKER, M. REIJANS, T. VAN DE LEE, M. HORNES, A. FRIJTERS, J. POT, J. PELEMAN, M. KUIPER and M. ZABEAU (1995): AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23: 4407-4414.10.1093/nar/23.21.44073073977501463Open DOISearch in Google Scholar

WILCOX, P. L., H. V. AMERSON, E. G. KUHLMAN, B. H. LIU, D. M. O. MALLEY and R. R. SEDEROFF (1996) Detection of a major gene for resistance to fusiform rust disease in loblolly pine by genomic mapping. Proc Natl Acad Sci 93: 3859-3864.10.1073/pnas.93.9.3859394498632980Open DOISearch in Google Scholar

WILLEMSEN, V., H. WOLKENFELT, G. VRIEZE, P. WEISBEEK and B. SCHERES (1998): The HOBBIT gene is required for formation of the root meristem in the Arabidopsis embryo. Development 125: 521-531.10.1242/dev.125.3.5219425146Search in Google Scholar

ZHANG, D., Z. ZHANG, W. SONG and K. YANG (2003). Optimizing segregation population selection for genetic linkage maps in Populus tomentosa Carr.. Journal of Beijing Forestry University 25: 22-25.Search in Google Scholar

ZHANG, D., Z. ZHANG, K. YANG and B. LI (2004): Genetic mapping in (Populus tomentosa x P. bolleana) and P. tomentosa Carr. using AFLP markers. Theor Appl Genet 108: 657-662.10.1007/s00122-003-1478-714564399Search in Google Scholar

ZHANG, Z. Y., Z. H. HUANG, D. F. ZHANG, F. W. SU, Y. H. ZHANG and F. X. DONG (1992): An investigation on flowering and bearing of clones in the arboretum of Populus tomentosa Carr.. Journal of Beijing Forestry University 14: 41-51.Search in Google Scholar

ZHU, Z. T. and Z. Y. ZHANG (1997): The status and advances of genetic improvement of Populus tomentosa Carr.. Journal of Beijing Forestry University (English Edition) 6: 1-7.Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo