1. bookVolume 55 (2006): Issue 1-6 (December 2006)
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Journal
eISSN
2509-8934
First Published
22 Feb 2016
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English
access type Open Access

Reduction of Lignin Content by Suppression of Expression of the LIM Domain Transcription Factor in Eucalyptus camaldulensis

Published Online: 19 Oct 2017
Volume & Issue: Volume 55 (2006) - Issue 1-6 (December 2006)
Page range: 269 - 277
Received: 26 Jul 2006
Accepted: 14 Oct 2006
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Abstract

We report a reduction of lignin content in the woody plant Eucalyptus camaldulensis by the suppression of gene expression of the LIM domain transcription factor. Previously, we identified a cDNA encoding the tobacco (Nicotiana tabacum) LIM domain transcription factor, Nt1iml, involved in lignin biosynthesis and that specifically binds to an important cis-acting element, the PALbox sequence. The orthologous Eucalyptus gene of Ntliml, namely Ecliml, was isolated from the E. camaldulensis cDNA library (84% amino acid identity). The antisense Ntliml construct with a kanamycin-resistant gene was introduced into E. camaldulensis. The transgenic Eucalyptus plants grown in the greenhouse showed decreased expression levels of severallignin biosynthesis genes, phenylalanine ammonialyase (PAL), cinnamate-4-hydroxylase (C4H) and 4-hydroxycinnamate CoA ligase (4CL). The abnormal phenotypic changes and a 29% reduction of lignin content were observed in the line LG12, in which the transcript level of Ecliml was most1y suppressed. Ec1iml is one of the key transcription factors involved in lignin biosynthesis.

Keywords

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