<abstract xmlns="http://www.w3.org/1999/xhtml"><p> We report a reduction of lignin content in the woody plant Eucalyptus camaldulensis by the suppression of gene expression of the LIM domain transcription factor. Previously, we identified a cDNA encoding the tobacco (Nicotiana tabacum) LIM domain transcription factor, Nt1iml, involved in lignin biosynthesis and that specifically binds to an important cis-acting element, the PALbox sequence. The orthologous Eucalyptus gene of Ntliml, namely Ecliml, was isolated from the E. camaldulensis cDNA library (84% amino acid identity). The antisense Ntliml construct with a kanamycin-resistant gene was introduced into E. camaldulensis. The transgenic Eucalyptus plants grown in the greenhouse showed decreased expression levels of severallignin biosynthesis genes, phenylalanine ammonialyase (PAL), cinnamate-4-hydroxylase (C4H) and 4-hydroxycinnamate CoA ligase (4CL). The abnormal phenotypic changes and a 29% reduction of lignin content were observed in the line LG12, in which the transcript level of Ecliml was most1y suppressed. Ec1iml is one of the key transcription factors involved in lignin biosynthesis.</p></abstract>

We report a reduction of lignin content in the woody plant Eucalyptus camaldulensis by the suppression of gene expression of the LIM domain transcription factor. Previously, we identified a cDNA encoding the tobacco (Nicotiana tabacum) LIM domain transcription factor, Nt1iml, involved in lignin biosynthesis and that specifically binds to an important cis-acting element, the PALbox sequence. The orthologous Eucalyptus gene of Ntliml, namely Ecliml, was isolated from the E. camaldulensis cDNA library (84% amino acid identity). The antisense Ntliml construct with a kanamycin-resistant gene was introduced into E. camaldulensis. The transgenic Eucalyptus plants grown in the greenhouse showed decreased expression levels of severallignin biosynthesis genes, phenylalanine ammonialyase (PAL), cinnamate-4-hydroxylase (C4H) and 4-hydroxycinnamate CoA ligase (4CL). The abnormal phenotypic changes and a 29% reduction of lignin content were observed in the line LG12, in which the transcript level of Ecliml was most1y suppressed. Ec1iml is one of the key transcription factors involved in lignin biosynthesis.

Reduction of Lignin Content by Suppression of Expression of the LIM Domain Transcription Factor in Eucalyptus camaldulensis

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

We report a reduction of lignin content in the woody plant Eucalyptus camaldulensis by the suppression of gene expression of the LIM domain transcription...

Reduction of Lignin Content by Suppression of Expression of the LIM Domain Transcription Factor in Eucalyptus camaldulensis

Published Online: Oct 19, 2017

Page range: 269 - 277

Received: Jul 26, 2006

Accepted: Oct 14, 2006

DOI: https://doi.org/10.1515/sg-2006-0035

Keywords
Eucalyptus camaldulensis, Ecliml gene, transgenic Eucalyptus, lignin, holocellulose

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Reduction of Lignin Content by Suppression of Expression of the LIM Domain Transcription Factor in Eucalyptus camaldulensis

Published Online: Oct 19, 2017

Page range: 269 - 277

Received: Jul 26, 2006

Accepted: Oct 14, 2006

DOI: https://doi.org/10.1515/sg-2006-0035

KeywordsEucalyptus camaldulensis, Ecliml gene, transgenic Eucalyptus, lignin, holocellulose

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
Eucalyptus camaldulensis, Ecliml gene, transgenic Eucalyptus, lignin, holocellulose