1. bookVolume 53 (2004): Issue 1-6 (December 2004)
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
access type Open Access

Evaluation of Clonal Variability in Shoot Coppicing Ability and in vitro Responses of Dalbergia sissoo Roxb

Published Online: 27 Oct 2017
Volume & Issue: Volume 53 (2004) - Issue 1-6 (December 2004)
Page range: 212 - 220
Received: 17 Feb 2004
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Summary

Clonal variations were observed amongst 12 clones of Dalbergia sissoo belonging to four states (U.P, Uttaranchal, Haryana and Rajasthan) of India, representing four different geographical zones in respect of ex vitro shoot coppicing ability and in vitro responses. Coppicing ability of shoot hedges of clones exhibited significant variation which ranged from average of 13.81 coppiced shoots (Clone 40, Uttar Pradesh) to 9.29 (Clone 64, Haryana). Comparative analysis of clones from different regions in respect to their coppicing ability revealed that clones from U.P had higher coppicing ability whereas those from Haryana proved to be least coppicers. Regional variations were also exhibited in the in vitro multiple bud induction ability on nodal explants excised from shoot hedges of clones (mean number of buds induced and percentage of cultures forming multiple buds). Regional as well as inter clonal variations were recorded in the shoot proliferation efficiency as well as rootability of microshoots of these clones as well as their optimal plant growth regulator requirements. BAP alone (2.5 μM) was sufficient for inducing multiple buds on cultured nodal explants of Uttaranchal and Uttar Pradesh region clones. On the contrary, clones from Rajasthan and Haryana had higher optimal requirement of BAP and in addition, they required media to be supplemented with auxin (NAA) for induction of multiple buds on explants. Correlation analysis between shoot coppicing ability of clones and in vitro performances of explants of these clones cultured on 2.5 μM BAP indicates a positive correlation. Observation lays credence to our view that these characters are genetically controlled and shoot coppicing can be used as a marker character in optimizing in vitro performance of clones. Using the information generated by this paper in vitro production of elite planting material can be maximized by ameliorating plant growth regulator requirement in the medium.

Keywords

AHUJA, M. R. (1983): Somatic cell differentiation and rapid clonal propagation of aspen. Silvae Genetica 32: 3-4.Search in Google Scholar

BERGMANN, B. A. and A. M. STOMP (1994): Effect of genotype on rooting of hypocotyls and in vitro-produced shoots of Pinus radiata. Plant Cell Tissue Org. Cult. 39: 195-202.Search in Google Scholar

BROWN, C. L. (1981): Application of tissue culture technology to production of woody biomass. National Swedish Board for Energy Source Development. IEA Report:NE 1981-18.Search in Google Scholar

COLEMAN, G. D. and S. G. ERNEST (1989): In vitro shoot regeneration of Populus deltoides: Effect of cytokinin and genotype. Plant Cell Rep. 8: 459-462.10.1007/BF00269048Search in Google Scholar

ERNEST, S. G. (1993): In vitro culture of pure species non-aspen poplars. In: Micropropagation of woody plants. AHUJA, M. R. (ed.). Kluwer Academic Publishers. Pp. 195-207.10.1007/978-94-015-8116-5_12Search in Google Scholar

GURUMURTHI, K.: Clonal forestry for tree propagation. In: Genetic Improvement and Propagation of Forest Trees. Eds. S. S. R. BENNET, K. SUBRAMANIAM, A. J. KURIAN. Pb: ICFRE, India. Pp 37-61.Search in Google Scholar

HAISSIG, B. E. and D. E. RIEMENSCHNEIDER (1988): Genetic effects on adventitious rooting. In: Adventitious root formation in cuttings. DAVIS, T. D., HAISSIG, B. E. and SANKHLA, N. (eds.). Dioscorides Press, Portland, Oregon. Pp. 47-60.Search in Google Scholar

HAMMERSCHLAG, F. (1982): Factors affecting establishment and growth of peach shoots in vitro. Hort. Sci. 17: 85-86.Search in Google Scholar

HARTMAN, H. T. and D. E. KESTER (1983): Plant Propagation. Principles and Practices. Prentice Hall, Inc. Englwood Cliffs, New Jersey.Search in Google Scholar

HORGAN, K. and L. HOLLAND (1989): Rooting micropropagated shoots from mature radiata pine. Can. J. For. Res. 19: 1309-1315.Search in Google Scholar

HORGAN, K. (1987): Pinus radiata. In: Cell and Tissue culture in Forestry. Vol. 3. Case histories: Gymnosperms, Angiosperms and Palms. BONGA, J. M. and DURZAN, D. J. (eds.) Martineuss Nijhoff Publ. Pp.: 128-145. 10.1007/978-94-017-0992-7_10Open DOISearch in Google Scholar

KACKAR, N. L., K. R. SOLANKI, S. C. VYAS and M. SINGH (1991): Micropropagation of Prosopis cineraria. Indian J. Exp. Biol. 29: 65-67.Search in Google Scholar

KALIA, S.: In vitro studies on morphogenesis and micropropagation of Dalbergia sissoo Roxb. and Dalbergia latifolia. PhD Thesis. Forest Research Institute, Dehradun, India. 2003.Search in Google Scholar

LEAKEY, R. R. B., A. C. NEWTON and J. MC P. DICK (1994): Capture of genetic variation by vegetative propagation processes determining successes. In: LEAKEY, R. R. B. and NEWTON, A. C. (eds.). London. HMSO: pp. 72-83.Search in Google Scholar

MURASHIGE, T. and F. SKOOG (1962): A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15: 473-497.Search in Google Scholar

NORTON, M. E. and C. R. NORTON (1985): In vitro propagation of Ericaceae: A comparison of the activity of cytokinins N6-benzyladenine and N6-isopentyl adenine in shoot proliferation. Sci. Hort. 27: 335-340.10.1016/0304-4238(85)90038-XSearch in Google Scholar

PAL, M., M. BAKSHI and R. PRAKASH (2003): Effect of coppicing height on shoot production capacity of different clones of Dalbergia sissoo Roxb. Indian Forester 504-508.Search in Google Scholar

PANETSOS, K. P., A. ECONOMOU and A. SCALTSOYIANNES (1987): Propagation in vitro of Aspen hybrid Populus spartiatica x Populus tremula from mature trees. Agricultural Research 11: 449-459.Search in Google Scholar

SCALTSOYIANNES, A., K. P. PANETSOS, A. ECONOMOU and P.TSOULPHA (1994): Micropropagation of the pine hybrid Pinus brutia (Ten.) x P. halepensis (Mill.) by culturing fascicle shoots. Ann. Sci. For. 51: 175-182.10.1051/forest:19940207Open DOISearch in Google Scholar

SHARMA, S. K. and S. KALIA (2004): Micropropagation of Eucalyptus tereticornis and interspecific hybrids. Current Advances in Plant Sciences (In Press).Search in Google Scholar

SHEKHAWAT, N. S., T. S. RATHORE, R. P. SINGH, N. S. DEORA and S. R. RAO (1993): Factors affecting in vitro clonal propagation of Prosopis cineraria. Plant Gr. Reg. 12: 273-280.Search in Google Scholar

SIMPSON, D.W. and J. A. BELL (1989): The response of different genotypes of Fragaria annanasa and their seedling progenies to in vitro micropropagation and the effect of varying the concentration of 6-benzylaminopurine in the proliferation medium. Plant Cell Tissue Organ Cult.17: 225-234.10.1007/BF00046869Open DOISearch in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo