Worldwide, breast cancer is the most commonly diagnosed female malignancy and the leading cause of cancer mortality in women.1,2 Radiotherapy (RT) is an essential component of multimodal breast cancer treatment. Adjuvant irradiation to the residual breast after wide local excision of the primary tumor provides equivalent outcomes to mastectomy while ensuring superior cosmesis.3,4,5 In patients with risk factors after mastectomy, adjuvant radiotherapy improves overall survival and local control.6,7 Due to improvements in breast cancer treatment, the number of long-term survivors has increased over the past decades.8 With larger numbers surviving, more patients become at risk of developing a wide range of late radiation-related side effects. Postoperative radiotherapy in patients with left-sided breast cancer is characterized by exposure of significant portion of the heart volume to high doses of irradiation.9 Pathophysiology of radiotherapy-induced cardiac toxicity involves damage of blood vessels and interstitial fibrosis, leading to coronary artery disease, valvular abnormalities, myocardial dysfunction, pericardial disease and conductive disturbances. These changes can become clinically manifest several years or even decades after treatment, leading to increased risk for cardiac morbidity and death.10,11,12,13,14,15,16,17,18,19,20,21,22,23,24 The increased risk is proportional to the dose received by the heart, begins within years after exposure and continues for decades.12 Various radiotherapy techniques, including breath hold (BH) during treatment have been proposed to reduce the cardiac dose.25,26 It is expected that these maneuvers will result in reduced probability of late clinical manifestations of cardiac events.12,27,28 Voluntary deep inspiration breath hold (V-DIBH) radiotherapy has been studied in the setting of breast conserving treatment and post-mastectomy.25,26 Using this technique, the distance from the chest wall to the heart increases during deep inspiration, resulting in a decrease of cardiac volume in the radiotherapy field. While consistency and stability of V-DIBH has been demonstrated, real-time positional monitoring is advocated in daily practice to apply corrective actions due to intra-fractional movements.29,30 In 2015, we implemented V-DIBH for patients undergoing leftsided breast cancer radiotherapy, based on a prospective observational study protocol. This implementation in routine practice was based on the existent body of evidence in favor of V-DIBH.25,26 We aimed to ensure controlled transition to the new routine technique and to report on feasibility of this approach in our clinical setting and its dosimetric impact. Our hypothesis was that V-DIBH, when compared with free-breathing (FB) technique, will result in statistically significant reduction of the commonly reported cardiac dose-volume histogram (DVH) parameters without compromising the coverage of the target volume with prescribed dose. We also hypothesized that V-DIBH treatment results in non-inferior set-up accuracy when compared with FB. Our study was approved by the institutional Medical Research Center (Study No. 15330/15), on 14. 10. 2015 by the Hamad Medical Corporation Medical Research Centre.
Formal calculation of the sample size was not performed. Instead, all consecutive left-sided breast cancer patients, treated at our department with postoperative radiotherapy between May 2015 and January 2017, were considered for V-DIBH. In this way, a clinically relevant patient cohort was enrolled during the period of V-DIBH implementation. Informed consent to treatment according to the protocol was obtained from all patients. Metastatic disease, compromised respiratory function, Eastern Cooperative Group performance status >2, need for sedation during treatment and patient refusal were exclusion criteria. Patients with cardiac V25Gy ≥ 5% on FB treatment plan were offered V-DIBH. In addition, selected cases with lower V25Gy were entered on the V-DIBH protocol. This selection was performed at discretion of the treating radiation oncologist by considering patient-related factors such as age, pre-existent ischemic cardiac events and other co-morbidities, application of cardio-toxic medications, history of smoking, diabetes mellitus, hyperlipidemia and arterial hypertension.
Our V-DIBH approach was adapted from the technique used in the UK HeartSpare study and was based on visual confirmation of the borders of the light fields, marked on the patient as surrogates of the isocenter.31 Planning scans were obtained with a 64-slice Siemens Somatom Definition CT scanner, using 5 mm slice thickness. During scanning, Patients were positioned supine with both arms above the head, using the breast board and knee rest (CIVCO Medical Solutions, © 2017 USA). Standard CT planning marks were used in all cases. They included four tattoos (superior, inferior, medial and lateral) and wires to delineate surgical scar and patient midline. First, FB CT was obtained. Following completion of FB CT, the patients, still in simulation position, underwent V-DIBH coaching. During coaching, patient’s ability to hold breath for the duration of treatment was checked. In addition, geometric consistency of BH was visually assessed using in-room lasers and skin marks. Finally, the patients were asked to practice V-DIBH technique overnight and returned the following day to complete V-DIBH CT scan. V-DIBH scans were completed using the same basic set up parameters as FB scans. During imaging, the therapists communicated with the patients by using in-room intercom. Video connection with closed-circuit camera was used for visual assessment of skin marks to ensure BH consistency; distance from tattoos to BH marks was recorded.
Contouring and treatment planning was performed with Varian External Beam Planning system, version 13.7 (© 1996-2016 Varian Medical Systems, Inc, Palo Alto, USA). Planning target volume (PTV), heart and lung were contoured on FB and V-DIBH CT scans by five different radiation oncologists according to our departmental contouring guidelines, adapted from the published recommendations.32 As per our departmental practice, internal mammary lymph node chains were not included in the treatment volume. Left anterior descending coronary artery (LAD) was contoured based on the recently published contouring atlas.33 Optimized PTV (OPTV) was created by subtracting 5 mm from PTV at the skin. Analysis of contouring uncertainties was limited to volumetric comparisons (mean volumes and relative standard deviations) of delineated regions of interest between FB and V-DIBH and within each approach.
Treatment planning was completed according to our institutional practice (see below) separately for FB and V-DIBH scans. Our standard prescription was 50 Gy in 25 fractions to the OPTV. When indicated, 45 Gy in 25 fractions were applied simultaneously with breast / chest wall irradiation to the ipsilateral supra-clavicular region. OPTV irradiation was followed by electron boost of 16 Gy in 8 fractions to lumpectomy bed or postmastectomy scar. Photon boost was not used. Dose contribution from electron boost was not included in the analysis. Dose optimization did not differ between FB and V-DIBH approach and consisted of forward-planned intensity modulated field in field technique. Subfields were fitted on a weighted pair of tangents to attain OPTV dose homogeneity. Planning aims were to cover ≥ 95% of the OPTV with ≥ 95% of prescribed dose and to keep the proportion of ipsilateral lung irradiated to 20 Gy (V20Gy) < 25%, cardiac V25Gy < 5% and mean cardiac dose < 5 Gy. For hypo-fractionation (40 Gy in 15 fractions), linear quadratic model with α/β of 3Gy was applied to calculate the biologically equivalent dose constraints for the lung (V18Gy < 22%–25%) and heart (V23Gy ≤ 5% and mean D ≤ 4Gy). DVH parameters of FB and V-DIBH treatment plans were recorded and compared.
For daily treatments, patients were positioned and immobilized identical to planning CT. BH consistency was checked in the room by using skin marks to reflect the measurements from CT and in-room lasers. Outside the treatment room, closed-circuit television camera was zoomed in to clearly define BH skin marks. Next, patients were asked to complete BH using the intercom system. Skin marks were visually checked through video connection to quantify inspiration and to ensure that the planned inspiration depth was reproduced during daily treatments. Set up verification and correction was performed through daily pre-port tangential megavoltage images and cine loop images (Varian On board imager 1.6 © 2015, Switzerland) to confirm both isocenter position and BH consistency. If corrections were required, they were applied in the room with the patient in BH position and new marks were placed on the patient’s skin. Set up errors were assessed offline for every fraction. For the first 18 patients treated with the V-DIBH technique, mean shifts and systematic and random errors were calculated. The results were compared with a retrospective cohort of patients treated at our department using the FB technique.
Mean values and standard deviations were used to present continuous numerical variables and paired sample t-test was used to test for significance of differences. Statistical tests were double sided with p-value of < 0.05 considered as the limit for significance. Miscrosoft Office Excel software was used for data analysis and statistics.
Sixty-three patients were considered for V-DIBH radiotherapy. Nine (14.3%) were excluded at the time of coaching due to non-compliance with the V-DIBH protocol. Additional two (3.2%) were unable to hold breath after the treatment start and reverted from V-DIBH to FB technique, but their DVH parameters are included in final analysis. In summary, the overall ability to complete radiotherapy using the V-DIBH technique was 82.5% and 54 cases were included in the DVH analysis. All patients were female and median age was 41 years (range: 30-64 years). The irradiated volume included residual breast after organ conserving surgery in 31 (57%) and chest wall after mastectomy in 23 (43%) cases. All patients received electron boost of 16 Gy in 8 fractions to the lumpectomy cavity or mastectomy scar. Left supra-clavicular region was included in the radiation fields in all 24 (100%) post-mastectomy and in 7 (22.6%) post-lumpectomy cases. Patients were required to perform 11 breath holds on an average daily treatment. Mean couch shifts and setup errors for the V-DIBH technique were not significantly different when compared with our historic cohort of patients, treated with FB approach (Table 1). Largest mean shift for initial patient setup was in longitudinal direction (2.7 mm), followed by the lateral (2.1 mm) and vertical direction (1.2 mm). Population systematic and random error was the highest for the longitudinal direction (2.1 and 0.7 mm, respectively).
Vertical, longitudinal and lateral couch shifts and corresponding systematic and random errors for the initial 18 patients treated with voluntary deep inspiration breath-hold (V-DIBH) and a historic control treated with free-breathing (FB) technique. Differences between the two approaches were non-significant. FB = free breathing; V-DIBH = voluntary deep inspiration breath holdVertical [mm] Longitudinal [mm] Lateral [mm] Mean shift 1 1.2 2.8 2.7 1.9 2.1 Systematic error 0.7 1.5 1.6 2.1 0.8 1.3 Random error 0.6 0.6 2.1 0.7 1.1 0.4
V-DIBH technique resulted in a significant increase of delineated left lung volume when compared with FB (1557 +/– 389 cm3
Impact of deep inspiration on cardiac dose during postoperative radiotherapy for left-sided breast cancer. Doses from 0% to three-dimensional dose maximum are depicted by color wash. Upper two slices are taken at mid-breast level and the lower pair 6 slices below. At both levels, the proportion of cardiac volume is reduced by voluntary deep inspiration breath-hold.Figure 1
Mean values +/– standard deviations of dose volume histogram (DVH) parameters for heart, left anterior descending coronary artery (LAD), lung and optimized planning target volume (OPTV) achieved by the free breathing and breath-hold technique. Dmax = maximal dose; Dmean = mean dose; Dx% = dose received by x% of volume; LAD = left anterior descending coronary artery; NS = non-significant; VxGy = relative volume, receiving a dose of x Gy; Vx% =relative volume, receiving x% of the prescribed doseDVH parameter Free breathing Breath-hold P-value Dmean [Gy] 6.1 +/– 2.5 3.2 +/– 1.4 Dmax [Gy] 51.1 +/– 1.4 48.5 +/– 6.8 V50Gy [%] 0.7 +/– 1.1 0.2 +/– 0.6 V40Gy [%] 6.2 +/– 3.5 2.1 +/– 1.9 V30Gy [%] 7.8 +/– 4 2.8 +/– 2.3 < 0.05 V25Gy [%] 8.5 +/– 4.2 3.2 +/– 2.5 V20Gy [%] 9.2 +/– 4.4 3.6 +/– 2.7 V15Gy [%] 10 +/– 4.6 4.1 +/– 2.9 V10Gy [%] 11.2 +/– 5 4.9 +/– 3.2 Dmean [Gy] 11.3 +/– 3.2 10.6 +/– 2.6 Dmax [Gy] 51.6 +/– 1.8 50.6 +/– 1.1 V30Gy [%] 17.9 +/– 6.3 17 +/– 4.6 NS V20Gy [%] 20.5 +/– 7 19.5 +/– 5.1 V10Gy [%] 25.9 +/– 8.1 25.4 +/– 5.8 Dmean [Gy] 23.0 +/– 6.7 14.8 +/– 7.6 Dmax [Gy] 49.7 +/– 3.4 44.3 +/– 12.2 < 0.05 D50% [Gy] 20.4 +/– 17.6 8.4 +/– 11.1 V95% [%] 95.6 +/– 4.1 95.2 +/– 6.3 NS
Impact of supraclavicular fossa (SCF) radiotherapy on heart and lung dose volume histogram (DVH) parameters NS = non-significantDVH parameter Free breathing p-value Breath hold p-value Dmean [Gy] 5.5 +/– 2.2 6.6 +/– 2.7 2.9 +/– 1.1 3.5 +/– 1.6 Dmax [Gy] 51.1 +/– 1.5 51.1 +/– 1.3 48.2 +/– 7.8 48.8 +/– 5.9 V50Gy [%] 1 +/– 1.3 0.5 +/– 0.9 0.3 +/– 0.7 0.2 +/– 0.3 V40Gy [%] 5.9 +/– 3.6 6.5 +/– 3.4 NS 1.6 +/– 1.4 2.4 +/– 2.2 NS V30Gy [%] 7.2 +/– 4.1 8.3 +/– 3.9 2.3 +/– 1.8 3.3 +/– 2.6 V25Gy [%] 7.8 +/– 4.2 9.1 +/– 4.1 2.6 +/– 1.9 3.7 +/– 2.8 V20Gy [%] 8.4 +/– 4.4 9.8 +/– 4.4 2.9 +/– 2.1 4.2 +/– 3 V15Gy [%] 9.2 +/– 4.7 10.6 +/– 4.5 3.4 +/– 2.3 4.7 +/– 3.2 V10Gy [%] 10.4 +/– 5.1 12 +/– 4.8 4.2 +/– 2.6 5.6 +/– 3.5 Dmean [Gy] 9 +/– 2.6 13.2 +/– 2.2 < 0.05 9.3 +/– 3.2 11.8 +/– 2.4 < 0.05 Dmax [Gy] 51.1 +/– 1.4 52 +/– 2.1 NS 50.4 +/– 1.1 50.8 +/– 1.1 NS V30Gy [%] 13.4 +/– 4.9 21.7 +/– 4.7 14 +/– 4 19.6 +/– 3.3 V20Gy [%] 15.5 +/– 5.3 24.9 +/– 5.1 < 0.05 16.3 +/– 4.4 22.3 +/– 3.8 < 0.05 V10Gy [%] 19.8 +/– 6.1 31.2 +/– 5.5 21.5 +/– 5.5 28.8 +/– 3.6
Adjuvant radiotherapy improves the treatment outcome after breast cancer surgery, but it also carries a risk for late cardiac toxicity in left sided tumors.3,4,5,6,7,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24 Various techniques were suggested to reduce this effect.25,26 In our present study, the implementation of V-DIBH technique achieved statistically significant reduction of cardiac doses without affecting the PTV coverage. V-DIBH radiotherapy was characterized by excellent patient compliance and high set-up accuracy which was non-inferior to the historic FB control.
We found that V-DIBH, when compared with FB technique, achieved significant reduction of all analyzed DVH parameters for LAD and heart (Table 2). In our patient cohort, the mean cardiac dose was reduced by 50% and V25Gy by 64% (Table 2). The heart-sparing effect afforded by the V-DIBH did not come at a cost of inferior dose-coverage of the OPTV when compared with the FB treatment plans (Table 2). Covariates that impact the cardiac dose during radiotherapy include the radiotherapy technique (intensity modulated vs. 3D conformal), use of cardiac sparing techniques, patient position, regional irradiation and boost.34 It should be emphasized that direct comparisons between different studies and our current report are challenging due to differences in the listed factors, including treatment volumes and heart sparing methodologies used. Hjelstuen
In patients without previous cardiac events, we used the heart V25Gy ≥ 5% as threshold for inclusion on the V-DIBH protocol. But the evidence suggests that there is no threshold dose below which the late cardiac effects do not occur. A recent study by Darby
Average cardiac volume as delineated on V-DIBH scans (547 +/–91 cm3) was significantly smaller when compared with FB scans (567 +/– 82 cm3) (p = 0.016), which could be attributed to the physiologic effect of V-DIBH. While statistically significant, the absolute average difference between contoured volumes was small (20 cm3), but there was a substantial spread of individual differences (st. dev: 61 cm3), indicating large variation between FB and V-DIBH based cardiac volumes in individual study cases. Contouring variation is one of the major sources of uncertainties in radiotherapy. In spite of the use of contouring guidelines, high quality imaging and participation of trained observers, complete elimination of contouring variation is an unrealistic aim.42 Therefore, the magnitude of contouring variation needs to be quantified and considered during the interpretation of DVH results. While the DVH parameters reported in our present study were based on relative cardiac volumes, the mean absolute volume was smaller on V-DIBH when compared with FB. Therefore, cardiac sparing afforded by V-DIBH technique is likely to be an underestimation of the actual sparing effect. This can be regarded as a supporting argument for the confirmation of our study hypothesis. The inter-observer (intra-approach) variation of cardiac contouring was comparable between the FB and V-DIBH scans as demonstrated by similar relative standard deviation of around 15%. Consequently, the impact of contouring variation on uncertainties of the DVH parameters was similar for both simulation approaches, making our results clinically relevant.
Our V-DIBH technique requires no additional equipment, ensures precise setup and is feasible, as demonstrated by the 82.5% compliance with coaching instructions. We found that the mean shifts for initial patient setup with V-DIBH technique were from 1.2 to 2.8 mm and the systematic and random setup errors were ≤ 2.1 mm. These favorable results did not differ significantly from our past experience with FB approach (Table 1). Real-time monitoring ensured high precision setup throughout the beam-on time. Although not formally analyzed, our experience with the V-DIBH confirms excellent reproducibility, constancy and stability of this treatment technique.29,30 Our results compare favorably with reports by other authors using similar approaches. The HeartSpare study was a randomized comparison between V-DIBH and deep-inspiratory breath-hold with Active Breathing Coordinator™ (ABC-DIBH; © Elekta).31 Twenty three patients were randomized to receive one technique for the first seven and the second technique for the following 8 fractions of hypofractionated regimen delivering 40 Gy in 15 fractions. Differences between V-DIBH and ABC-DIBH based setup errors were non-significant. Systematic errors derived from electronic portal imaging were ≤ 1.8 mm for v-DIBH and ≤ 2 mm for ABC-DIBH, while the respective random errors were ≤ 2.5 mm and ≤ 2.2 mm. Furthermore, V-DIBH was preferred by patients and staff, took less time to deliver and achieved similar normal tissue sparing at lower cost when compared with the ABC-DIBH.31 Similarly, Borst
When compared with free-breathing radiotherapy, voluntary deep inspiration breath-hold enabled significant reduction of cardiac doses without compromising the target volume coverage in our cohort of left-sided breast cancer patients. Treatment setup shifts and population systematic and random errors were small and not significantly different from the historic controls treated with the free-breathing technique. Our systematic approach to patient coaching was reflected in low rate of conversion to free-breathing radiotherapy. Based on the positive results of our present study and experience gained, we adopted V-DIBH radiotherapy as standard treatment for all left-sided breast cancer patients. Long follow up is needed to confirm the clinical impact of the presented favorable dosimetric benefit.