<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The extracellular polymeric substances (EPS) produced by the bacteria associated with the gastropod <italic>Turbo</italic> sp. were isolated and screened for antibacterial activity against biofilm-forming bacteria. EPS of five out of 13 strains showed inhibitory activities in the antibacterial assay. Furthermore, the antibiofilm and antifouling activity of the most active EPS isolated from the strain KT1 was studied using various <italic>in vitro</italic> and <italic>in vivo</italic> bioassays. Results revealed that EPS significantly inhibited the growth and biofilm formation of bacteria. Furthermore, the antifouling coating developed with bacterial EPS considerably reduced the recruitment of fouling organisms on coated surfaces submerged in the seawater. The functional groups present in EPS, characterized by strong activity, were analyzed using FT-IR and the spectrum showed the presence of alcohol, amines, carboxylic acid and esters. The bacterium responsible for the production of bioactive EPS was identified as <italic>Pseudomonas aeruginosa</italic>, using the 16S rRNA gene. Since the findings of this study revealed the antibiofilm and antifouling activities of EPS, further long term field tests and characterization of the bioactive compound of the EPS could lead to the development of eco-friendly antifouling coating.</p>
</abstract>

The extracellular polymeric substances (EPS) produced by the bacteria associated with the gastropod Turbo sp. were isolated and screened for antibacterial activity against biofilm-forming bacteria. EPS of five out of 13 strains showed inhibitory activities in the antibacterial assay. Furthermore, the antibiofilm and antifouling activity of the most active EPS isolated from the strain KT1 was studied using various in vitro and in vivo bioassays. Results revealed that EPS significantly inhibited the growth and biofilm formation of bacteria. Furthermore, the antifouling coating developed with bacterial EPS considerably reduced the recruitment of fouling organisms on coated surfaces submerged in the seawater. The functional groups present in EPS, characterized by strong activity, were analyzed using FT-IR and the spectrum showed the presence of alcohol, amines, carboxylic acid and esters. The bacterium responsible for the production of bioactive EPS was identified as Pseudomonas aeruginosa, using the 16S rRNA gene. Since the findings of this study revealed the antibiofilm and antifouling activities of EPS, further long term field tests and characterization of the bioactive compound of the EPS could lead to the development of eco-friendly antifouling coating.

Antibiofilm and antifouling activities of extracellular polymeric substances isolated from the bacteria associated with marine gastropod <italic>Turbo</italic> sp

Department of Marine Biology, Faculty of Marine Sciences

Oceanological and Hydrobiological Studies

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Antibiofilm and antifouling activities of extracellular polymeric substances isolated from the bacteria associated with marine gastropod  sp

The extracellular polymeric substances (EPS) produced by the bacteria associated with the gastropod Turbo sp. were isolated and screened for antibacterial...

Antibiofilm and antifouling activities of extracellular polymeric substances isolated from the bacteria associated with marine gastropod Turbo sp

Article Category: Original research paper

Published Online: Mar 10, 2016

Page range: 11 - 19

Received: Apr 12, 2015

Accepted: Aug 17, 2015

DOI: https://doi.org/10.1515/ohs-2016-0002

Keywords
biofouling, surface associated bacteria, extracellular polymeric substance, antimicrobial activity, antibiofilm activity, antifouling activity

© Faculty of Oceanography and Geography, University of Gdañsk, Poland. All rights reserved.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Antibiofilm and antifouling activities of extracellular polymeric substances isolated from the bacteria associated with marine gastropod Turbo sp

Article Category: Original research paper

Published Online: Mar 10, 2016

Page range: 11 - 19

Received: Apr 12, 2015

Accepted: Aug 17, 2015

DOI: https://doi.org/10.1515/ohs-2016-0002

Keywordsbiofouling, surface associated bacteria, extracellular polymeric substance, antimicrobial activity, antibiofilm activity, antifouling activity

© Faculty of Oceanography and Geography, University of Gdañsk, Poland. All rights reserved.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
biofouling, surface associated bacteria, extracellular polymeric substance, antimicrobial activity, antibiofilm activity, antifouling activity