<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The investigations covered a total of 234 lungs from necropsied pigs with different pneumonic lesions, from 6 farrow-to-finish pig farms during 2013 and 2014. The samples were inoculated on selective culture media and aerobically incubated at 37°C and in carbon dioxide condition. The isolated bacterial colonies were further characterised morphologically and biochemically. The identification was confirmed using the BBL Crystal, E/N, G/P ID Kit (Becton Dickinson). For determination of the type of <italic>Pasteurella multocida</italic>, the PCR method was used. The findings showed that bacteria were isolated from 202 (86%) out of 234 examined lung samples. The pure isolates of <italic>Pasteurella multocida</italic> were obtained from 71 (35 %) samples. Out of the remaining 29 (14%) examined lung samples, 9, 8, 7 and 5 examined lung samples were shown as mixed cultures of <italic>Pasteurella multocida</italic> and <italic>Streptococcus</italic> spp., <italic>Arcanobacterium pyogenes</italic>, <italic>Actinobacillus pleuropneumoniae</italic> and <italic>Haemophilus parasuis</italic>, respectively. The PCR method confirmed that all 15 investigated strains of <italic>P. multocida</italic> belong to type A.</p></abstract>

The investigations covered a total of 234 lungs from necropsied pigs with different pneumonic lesions, from 6 farrow-to-finish pig farms during 2013 and 2014. The samples were inoculated on selective culture media and aerobically incubated at 37°C and in carbon dioxide condition. The isolated bacterial colonies were further characterised morphologically and biochemically. The identification was confirmed using the BBL Crystal, E/N, G/P ID Kit (Becton Dickinson). For determination of the type of Pasteurella multocida, the PCR method was used. The findings showed that bacteria were isolated from 202 (86%) out of 234 examined lung samples. The pure isolates of Pasteurella multocida were obtained from 71 (35 %) samples. Out of the remaining 29 (14%) examined lung samples, 9, 8, 7 and 5 examined lung samples were shown as mixed cultures of Pasteurella multocida and Streptococcus spp., Arcanobacterium pyogenes, Actinobacillus pleuropneumoniae and Haemophilus parasuis, respectively. The PCR method confirmed that all 15 investigated strains of P. multocida belong to type A.

The Prevalence of <italic>Pasteurella Multocida</italic> from Farm Pigs in Serbia

Institute of Veterinary Medicine of Serbia

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The Prevalence of  from Farm Pigs in Serbia

The investigations covered a total of 234 lungs from necropsied pigs with different pneumonic lesions, from 6 farrow-to-finish pig farms during 2013 and 2014....

The Prevalence of Pasteurella Multocida from Farm Pigs in Serbia

Published Online: Mar 15, 2016

Page range: 39 - 42

Received: Jul 20, 2015

Accepted: Oct 19, 2015

DOI: https://doi.org/10.1515/macvetrev-2015-0063

Keywords
lung, pig

© 2015 Oliver Radanovic et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The Prevalence of Pasteurella Multocida from Farm Pigs in Serbia

Published Online: Mar 15, 2016

Page range: 39 - 42

Received: Jul 20, 2015

Accepted: Oct 19, 2015

DOI: https://doi.org/10.1515/macvetrev-2015-0063

Keywordslung, pig

© 2015 Oliver Radanovic et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
lung, pig