<abstract xmlns="http://www.w3.org/1999/xhtml"><p>During a nematode biodiversity survey from 2012 to 2014 in Shenzhen, China, ten nematode populations (SZX1301–SZX1310) of <italic>Xiphinema</italic> were recovered from rhizosphere of different plants, namely <italic>Acacia mangium</italic> (SZX1306), <italic>A. confuse</italic> (SZX1309), <italic>Blechnum orientale</italic> (SZX1301, SZX1302, SZX1307, SZX1308), <italic>Litchi chinensis</italic> (SZX1304, SZX1310) in Tianxinshan and <italic>Gleichenia linearis</italic> (SZX1303, SZX1305) in Yangmeikeng environmental monitoring sites. Morphological and molecular profiles of these populations were determined. Three species of <italic>Xiphinema</italic>, i.e., <italic>X. hunaniense</italic><xref ref-type="bibr" rid="j_helmin-2015-0068_ref_063_w2aab3b7c30b1b6b1ab2b1c63Aa">Wang &amp; Wu, 1992</xref>, <italic>X. brasiliense</italic><xref ref-type="bibr" rid="j_helmin-2015-0068_ref_040_w2aab3b7c30b1b6b1ab2b1c40Aa">Lordello, 1951</xref> and <italic>X. americanum</italic> Cobb, 1913 <italic>sensu lato</italic> were identified using morphological characters and molecular data of partial 18S and 28S D2–D3 rDNA expansion segments. Four populations (SZX1301–SZX1304) were <italic>X. hunaniense</italic>, one population (SZX1305) <italic>X. brasiliense</italic>, and five populations (SZX1306–SZX1310) <italic>X. americanum s.l</italic>.. Phylogenetic analysis based on sequences of the 28S rDNA D2–D3 expansion segment revealed these three species are all distinct species and supported a close relationship with their corresponding species. This is the first report of <italic>X. hunaniense, X. brasiliense</italic> and <italic>X. americanum s.l</italic>. in their hosts except for <italic>L. chinensis</italic>.</p></abstract>

During a nematode biodiversity survey from 2012 to 2014 in Shenzhen, China, ten nematode populations (SZX1301–SZX1310) of Xiphinema were recovered from rhizosphere of different plants, namely Acacia mangium (SZX1306), A. confuse (SZX1309), Blechnum orientale (SZX1301, SZX1302, SZX1307, SZX1308), Litchi chinensis (SZX1304, SZX1310) in Tianxinshan and Gleichenia linearis (SZX1303, SZX1305) in Yangmeikeng environmental monitoring sites. Morphological and molecular profiles of these populations were determined. Three species of Xiphinema, i.e., X. hunanienseWang & Wu, 1992, X. brasilienseLordello, 1951 and X. americanum Cobb, 1913 sensu lato were identified using morphological characters and molecular data of partial 18S and 28S D2–D3 rDNA expansion segments. Four populations (SZX1301–SZX1304) were X. hunaniense, one population (SZX1305) X. brasiliense, and five populations (SZX1306–SZX1310) X. americanum s.l.. Phylogenetic analysis based on sequences of the 28S rDNA D2–D3 expansion segment revealed these three species are all distinct species and supported a close relationship with their corresponding species. This is the first report of X. hunaniense, X. brasiliense and X. americanum s.l. in their hosts except for L. chinensis.

Morphological and molecular characterization of <italic>Xiphinema</italic> species from Shenzhen, China

Department of Plant Protection, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People’s Republic of China

Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695–7613, USA

Nematode Assay Section, Agronomic Division, North Carolina Department of Agriculture and Consumer Services, Raleigh, NC 27607, USA

Shenzhen Environmental Monitoring Center, Shenzhen 518049, People’s Republic of China

College of Life Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People’s Republic of China

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Morphological and molecular characterization of  species from Shenzhen, China

During a nematode biodiversity survey from 2012 to 2014 in Shenzhen, China, ten nematode populations (SZX1301–SZX1310) of Xiphinema were recovered from...

Morphological and molecular characterization of Xiphinema species from Shenzhen, China

Article Category: Research Article

Published Online: Mar 10, 2016

Page range: 62 - 75

Received: May 12, 2015

Accepted: Aug 31, 2015

DOI: https://doi.org/10.1515/helmin-2015-0068

Keywords
spp., 28S rDNA, dagger nematode

© 2016 Institute of Parasitology, SAS, Košice

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Morphological and molecular characterization of Xiphinema species from Shenzhen, China

Article Category: Research Article

Published Online: Mar 10, 2016

Page range: 62 - 75

Received: May 12, 2015

Accepted: Aug 31, 2015

DOI: https://doi.org/10.1515/helmin-2015-0068

Keywordsspp., 28S rDNA, dagger nematode

© 2016 Institute of Parasitology, SAS, Košice

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
spp., 28S rDNA, dagger nematode