Heavy metal intoxication compromises the host cytokine response in Ascaris Suum model infection
Article Category: Research Article
Published Online: Mar 10, 2016
Page range: 14 - 23
Received: Oct 13, 2015
Accepted: Nov 10, 2015
DOI: https://doi.org/10.1515/helmin-2015-0063
Keywords
© 2016 Institute of Parasitology, SAS, Košice
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
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Fig. 3.
Parasite larval burden in mice intoxicated with heavy metals (lead Pb, cadmium Cd, mercury Hg) and infected with A. suum
| Heavy Metal Intoxication | Days of experiment (Days post infection) | intoxication + | |||
|---|---|---|---|---|---|
| Liver (mean±S.D.) | lungs (mean±S.D.) | lungs (mean±S.D.) | lungs (mean±S.D.) | ||
| Pb | 25. (4. p.i.) | 195.0 ± 43.0 | 250.6 ± 28.7 | ||
| 28. (7. p.i.) | 3.3 ± 1.5 | 66.7 ± 15.9 | 2.6 ± 3.8 | 86.3 ± 17.5 | |
| Cd | 25. (4. p.i.) | 225.5 ± 57.3 | P<0.05) statistically significant differences between | ||
| 28. (7. p.i.) | 7.3 ± 3.5 | 31.7 ± 23.6 | 2.3 ± 4.0 | 33.3 ± 2.1 | |
| Hg | 25. (4. p.i.) | 179.3 ± 40.5 | P<0.05) statistically significant differences between | ||
| 28. (7. p.i.) | 4.3 ± 3.2 | 25.7 ± 6.1 | 4.3 ± 4.2 | P<0.05) statistically significant differences between | |
Heavy metal concentrations in tissues of mice intoxicated with heavy metals (lead Pb, cadmium Cd, mercury Hg) and infected with A. suum
| Heavy metal | Experimental groups | Days of experiment (Days post infection) | liver (mg/kg) (mean±S.D.) | kidneys (mg/kg) (mean±S.D.) | Muscles (mg/kg) (mean±S.D.) |
|---|---|---|---|---|---|
| Pb | control | 0 | 0.12 ± 0.06 | 0.22 ± 0.08 | 0.16 ± 0.09 |
| intoxication (before infection) | 21 | 0.51 ± 0.18 | (P<0.05; | 0.71 ± 0.18 | |
| intoxicated | 35 | 0.27 ± 0.03 | 0.34 ± 0.04 | 0.48 ± 0.04 | |
| intoxicated+infected | 35 (14 p.i.) | 0.36 ± 0.14 | 0.44 ± 0.02 | 0.59 ± 0.14 | |
| Cd | control | 0 | 0.02 ± 0.01 | 0.05 ± 0.01 | 0.03 ± 0.01 |
| intoxication (before infection) | 21 | (P<0.01); | (P<0.01); | 0.04 ± 0.01 | |
| intoxicated | 35 | (P<0.05; | (P<0.0001) statistically significant differences between control and heavy metal’s intoxication; | 0.03 ± 0.01 | |
| intoxicated+infected | 35 (14 p.i.) | 0.35 ± 0.30 | (P<0.05) statistically significant differences between heavy metal’s intoxication and heavy metal’s intoxication in combination with | 0.05 ± 0.01 | |
| Hg | control | 0 | 1.03 ± 0.35 | 0.98 ± 0.39 | 0.03 ± 0.01 |
| intoxication (before infection) | 21 | (P<0.0001) statistically significant differences between control and heavy metal’s intoxication; | (P<0.0001) statistically significant differences between control and heavy metal’s intoxication; | (P<0.01); | |
| intoxicated | 35 | (P<0.0001) statistically significant differences between control and heavy metal’s intoxication; | (P<0.0001) statistically significant differences between control and heavy metal’s intoxication; | (P<0.01); | |
| intoxicated+infected | 35 (14 p.i.) | 9.57 ± 4.12 | 90.68±1.57 | 0.52±0.18 |