<abstract xmlns="http://www.w3.org/1999/xhtml"> The present study has described a simple protocol for efficient plant regeneration of Asparagus densiflorus ‘Sprengeri’ and ‘Myriocladus’ using single-node spear explants, and indirect organogenesis via callogenesis induced on internode explants. The results showed that the genotypes ‘Sprengeri’ and ‘Myriocladus’ regenerated to complete plants via nodal cultures and callus tissue, but the plant regeneration response was higher in secondary explants on MS medium with NAA + kinetin (1+1 mg dm-3) after transfer onto a multiplication medium with IAA+BAP (1+4 mg dm-3), and then onto a rooting medium supplemented with IBA (10 mg dm-3) or NAA + kinetin (1+1 mg dm-3). Primary explants of both cultivars showed high regenerative potential (via the callus stage) on MS medium with IAA+BAP. The cultivar Sprengeri also regenerated via somatic embryogenesis. Both kinds of ‘Meyeri’ explants have a morphogenetic potential for the formation of shoots, which, however, were not capable of rooting. This confirms that the explant, genotype and culture medium are determining factors in the in vitro plant regeneration system.</abstract>

The present study has described a simple protocol for efficient plant regeneration of Asparagus densiflorus ‘Sprengeri’ and ‘Myriocladus’ using single-node spear explants, and indirect organogenesis via callogenesis induced on internode explants. The results showed that the genotypes ‘Sprengeri’ and ‘Myriocladus’ regenerated to complete plants via nodal cultures and callus tissue, but the plant regeneration response was higher in secondary explants on MS medium with NAA + kinetin (1+1 mg dm-3) after transfer onto a multiplication medium with IAA+BAP (1+4 mg dm-3), and then onto a rooting medium supplemented with IBA (10 mg dm-3) or NAA + kinetin (1+1 mg dm-3). Primary explants of both cultivars showed high regenerative potential (via the callus stage) on MS medium with IAA+BAP. The cultivar Sprengeri also regenerated via somatic embryogenesis. Both kinds of ‘Meyeri’ explants have a morphogenetic potential for the formation of shoots, which, however, were not capable of rooting. This confirms that the explant, genotype and culture medium are determining factors in the in vitro plant regeneration system.

Micropropagation of Asparagus densiflorus via axillary shoots, indirect organogenesis and somatic embryogenesis

Folia Horticulturae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The present study has described a simple protocol for efficient plant regeneration of Asparagus densiflorus ‘Sprengeri’ and ‘Myriocladus’ using single-node...

Micropropagation of Asparagus densiflorus via axillary shoots, indirect organogenesis and somatic embryogenesis

Published Online: Dec 29, 2017

Page range: 143 - 153

Received: Apr 11, 2017

Accepted: Jul 19, 2017

DOI: https://doi.org/10.1515/fhort-2017-0014

Keywords
ornamental asparagus, micropropagation, somatic embryogenesis

© by Anna Pindel

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Micropropagation of Asparagus densiflorus via axillary shoots, indirect organogenesis and somatic embryogenesis

Published Online: Dec 29, 2017

Page range: 143 - 153

Received: Apr 11, 2017

Accepted: Jul 19, 2017

DOI: https://doi.org/10.1515/fhort-2017-0014

Keywordsornamental asparagus, micropropagation, somatic embryogenesis

© by Anna Pindel

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
ornamental asparagus, micropropagation, somatic embryogenesis