<abstract xmlns="http://www.w3.org/1999/xhtml"><p> In the paper, the identification and quantification of α- and β-amyrin in five varieties of Humulus lupulus is described. The plant samples were extracted with ethyl acetate and analyzed using of high performance liquid chromatography (HPLC) with PAD detection. The separation was achieved on RP 18 column, at 2 mL/min flow rate and at temperature of 35°C. Acetonitrile was used as a mobile phase. The established calibration curves and the other validation parameters: linearity (correlation coefficient r > 0.9988) and precision (RSD values ranged from 0.14 to 1.81%) were found to be satisfactory for the proposed method. The content of α- and β-amyrin strongly depended on varieties of H. lupulus and amounted from 72.7 to 232.5 μg/g and from 77.9 to 176.9 μg/g of dry plant material, respectively.</p></abstract>

In the paper, the identification and quantification of α- and β-amyrin in five varieties of Humulus lupulus is described. The plant samples were extracted with ethyl acetate and analyzed using of high performance liquid chromatography (HPLC) with PAD detection. The separation was achieved on RP 18 column, at 2 mL/min flow rate and at temperature of 35°C. Acetonitrile was used as a mobile phase. The established calibration curves and the other validation parameters: linearity (correlation coefficient r > 0.9988) and precision (RSD values ranged from 0.14 to 1.81%) were found to be satisfactory for the proposed method. The content of α- and β-amyrin strongly depended on varieties of H. lupulus and amounted from 72.7 to 232.5 μg/g and from 77.9 to 176.9 μg/g of dry plant material, respectively.

Identificatin and quantiative analysis of amyrins in Humulus lupulus L.

Departament of Chemistry, Medicinal University in Lublin, Chodzki 4a, 20-093 Lublin

Current Issues in Pharmacy and Medical Sciences

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{"article-title":"Identificatin and quantiative analysis of amyrins in Humulus lupulus L."}

In the paper, the identification and quantification of α- and β-amyrin in five varieties of Humulus lupulus is described. The plant samples were extracted with...

Identificatin and quantiative analysis of amyrins in Humulus lupulus L.

Published Online: Mar 03, 2015

Page range: 142 - 144

Received: Aug 20, 2014

Accepted: Aug 26, 2014

DOI: https://doi.org/10.1515/cipms-2015-0002

Keywords
amyrin, Humulus lupulus, triterpenes

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Identificatin and quantiative analysis of amyrins in Humulus lupulus L.

Published Online: Mar 03, 2015

Page range: 142 - 144

Received: Aug 20, 2014

Accepted: Aug 26, 2014

DOI: https://doi.org/10.1515/cipms-2015-0002

Keywordsamyrin, Humulus lupulus, triterpenes

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
amyrin, Humulus lupulus, triterpenes