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Polymorphisms of α1-antitrypsin and Interleukin-6 genes and the progression of hepatic cirrhosis in patients with a hepatitis C virus infection

T Motawi

T Motawi

Department of Biochemistry, Faculty of Pharmacy, Cairo UniversityCairo, Egypt
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Motawi, T
, 
OG Shaker

OG Shaker

Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo UniversityCairo, Egypt
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Shaker, OG
, 
RM Hussein

RM Hussein

Department of Biochemistry, Faculty of Pharmacy, Beni-Suef UniversityBeni-Suef, Egypt
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Hussein, RM
 and 
M Houssen

M Houssen

Department of Biochemistry, Faculty of Pharmacy, Damanhour UniversityDamanhour, Egypt
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Houssen, M
  
Dec 31, 2016
Polymorphisms of α1-antitrypsin and Interleukin-6 genes and the progression of hepatic cirrhosis in patients with a hepatitis C virus infection's Cover Image
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Article Category: Original Article
Published Online: Dec 31, 2016
Page range: 35 - 44
DOI: https://doi.org/10.1515/bjmg-2016-0034
Keywords
© 2016 Motawi T, Shaker OG, Hussein RM, Houssen M
This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
The A1AT and IL-6 genotypes by PCR-RFLP. The left panel shows the M, S and Z alleles of the A1AT genotypes. M: DNA marker; ZZ genotype (179 and 100 bp band); SZ genotype (179, 157, 121 and 100 bp bands); MM genotype (157 and 100 bp bands); SM genotype (157, 121 and 100 bp bands). The right panel shows the G and C alleles of the IL-6 genotypes. GC genotype (168, 119 and 49 bp); GG genotype (168 bp band); CC genotype (119 and 49 bp bands); M: DNA marker.
The A1AT and IL-6 genotypes by PCR-RFLP. The left panel shows the M, S and Z alleles of the A1AT genotypes. M: DNA marker; ZZ genotype (179 and 100 bp band); SZ genotype (179, 157, 121 and 100 bp bands); MM genotype (157 and 100 bp bands); SM genotype (157, 121 and 100 bp bands). The right panel shows the G and C alleles of the IL-6 genotypes. GC genotype (168, 119 and 49 bp); GG genotype (168 bp band); CC genotype (119 and 49 bp bands); M: DNA marker.

The clinical characteristics and biochemical parameters of the control group, chronic hepatitis patients and cirrhotic patients_

ParametersControl (n = 100)Chronic Hepatitis Patients (n= 85)Cirrhotic Patients (n = 65)
SexesM: 45; F: 55M: 55; F: 30M: 35; F: 30
Age (years ± SE)33.10 ± 1.8338.88 ± 1.9238.92 ± 2.36
Serum ALT (IU/mL)30.00 ± 1.3483.53 ± 16.03

significant values (p <0.05) when compared to the control group.

110.00 ± 14.63

significant values (p <0.05) when compared to the control group.

Serum AST (IU/mL)30.20 ± 1.3676.76 ± 11.07

significant values (p <0.05) when compared to the control group.

172.92 ± 27.76

significant values (p <0.05) when compared to the control group.

,

significant values (p <0.05) between chronic hepatitis and cirrhotic patients.

Serum ALP (IU/mL)43.65 ± 1.69113.71 ± 6.51

significant values (p <0.05) when compared to the control group.

119.85 ± 9.49

significant values (p <0.05) when compared to the control group.

Total bilirubin (mg/dL)0.75 ± 0.0450.92 ± 0.0981.09 ± 0.14

significant values (p <0.05) when compared to the control group.

Direct bilirubin (mg/dL)0.15 ± 0.010.25 ± 0.060.28 ± 0.05
Albumin (g/dL)3.85 ± 0.053.69 ± 0.053.21 ± 0.11

significant values (p <0.05) when compared to the control group.

,

significant values (p <0.05) between chronic hepatitis and cirrhotic patients.

Prothrombin time (seconds)11.19 ± 0.1411.53 ± 0.1913.46 ± 0.51

significant values (p <0.05) when compared to the control group.

,

significant values (p <0.05) between chronic hepatitis and cirrhotic patients.

AFP (ng/mL)5.86 ± 0.458.76 ± 0.5527.38 ± 6.91

significant values (p <0.05) when compared to the control group.

,

significant values (p <0.05) between chronic hepatitis and cirrhotic patients.

Fibrosis score (1-6)—1.94 ± 0.184.46 ± 0.14

significant values (p <0.05) when compared to the control group.

The single nucleotide polymorphism pattern of the A1TAT gene in the control group, chronic hepatitis patients and cirrhotic patients_

ParametersControl Group n (%)Chronic Hepatitis Patients n (%)Cirrhotic Patients n (%)
Genotypes:
 MM85 (85.0)40 (47.0)35 (53.8)

significant values (p <0.05) when compared to the control group.

 MS10 (10.0)30 (35.3)15 (23.1)
 MZ5 (5.0)5 (5.9)0 (0.0)
 ZZ0 (0.0)10 (11.8)0 (0.0)
 SS0 (0.00 (0.0)15 (23.1)
Alleles:
 M185 (92.5)115 (67.6)85 (56.4)

significant values (p <0.05) when compared to the control group.

 S10 (5.0)30 (17.6)45 (34.6)

significant values (p <0.05) when compared to the control group.

 Z5 (2.5)25 (14.7)0 (0.0)

The biochemical parameters within different A1AT genotypes in the control group, chronic hepatitis patients and cirrhotic patients_

ParametersControl GroupChronic Hepatitis PatientsCirrhotic Patients
MMMS+MZMMMSMZ+ZZMMMS+SS
Serum ALT (IU/mL)30.2±1.528.7±2.665.4±6.967.7±13.20163.7±80.287.0±80.2176.3±43.4

significant values (p <0.05) when the MS+SS genotypes of cirrhotic patients were compared to the MM genotypes of cirrhotic patients.

Serum AST (IU/mL)30.1±1.631.0±2.568.0±5.657.3±10.50139.0±47.9

significant values (p <0.05) when the MZ+ZZ genotypes of chronic hepatitis patients were compared to the MM genotypes of chronic hepatitis patients.

significant values (p <0.05) when the MZ+ZZ genotypes of chronic hepatitis patients were compared to the MS genotypes of chronic hepatitis patients.

127.7±20.1300.7±65.9

significant values (p <0.05) when the MS+SS genotypes of cirrhotic patients were compared to the MM genotypes of cirrhotic patients.

Serum ALP (IU/mL)44.5±1.938.7±0.9109.3±7.4115.5±5.70122.0±34.5114.6±10.8130.7±34.8
Total bilirubin (mg/dL)0.8±0.10.7±0.20.8±0.070.9±0.101.3±0.50.9±0.11.8±0.4

significant values (p <0.05) when the MS+SS genotypes of cirrhotic patients were compared to the MM genotypes of cirrhotic patients.

Direct bilirubin (mg/dL)0.2±0.020.1±0.030.2±0.020.2±0.040.5±0.30.2±0.010.5±0.2

significant values (p <0.05) when the MS+SS genotypes of cirrhotic patients were compared to the MM genotypes of cirrhotic patients.

Albumin (g/dL)3.8±0.053.9±0.23.7±0.13.7±0.103.6±0.033.3±0.12.7±0.1

significant values (p <0.05) when the MS+SS genotypes of cirrhotic patients were compared to the MM genotypes of cirrhotic patients.

Prothrombin time (seconds)11.2±0.211.3±0.311.5±0.211.2±0.2012.3±0.913.7±0.615.0±0.6
AFP (ng/mL)6.0±0.55.0±1.08.6±0.98.7±1.009.3±1.025.6±10.348.3±11.2
Fibrosis score (1-6)——2.1±0.31.5±0.202.3±0.34.6±0.24.3±0.3

The single nucleotide polymorphism pattern of the IL-6 gene in the control group, chronic hepatitis patients and cirrhotic patients_

ParametersControl Group n (%)Chronic Hepatitis Patients n (%)Cirrhotic Patients n (%)
Genotypes:
 GG100 (100.0)60 (70.6)40 (61.5)

significant values (p <0.05) when compared to the control group.

 GC0 (0.0)20 (23.5)25 (38.5)
 CC0 (0.0)5 (5.9)0 (0.0)
Alleles:
 G200 (100.0)140 (82.4)105 (80.8)
 C0 (0.0)30 (17.6)25 (19.2)

significant values (p <0.05) when compared to the control group.

The biochemical parameters within different IL-6 genotypes in the control group, chronic hepatitis patients and cirrhotic patients_

ParametersGenotypes
Control GroupChronic Hepatitis PatientsCirrhotic Patients
GGGG+GCCCGGGC
Serum ALT (IU/mL)30.00±1.3480.75±7.09128.10±52.91102.63±17.87121.80±26.81
Serum AST (IU/mL)30.20±1.3673.33±5.61137.45±35.74150.50±28.65208.80±54.69
Serum ALP (IU/mL)43.65±1.69115.93±5.4578.34±19.49125.25±14.21111.20±10.31
Total bilirubin (mg/dL)0.75±0.0450.92±0.060.90±0.321.09±0.171.00±0.28
Direct bilirubin (mg/dL)0.15±0.010.26±0.020.20±0.190.29±0.070.26±0.09
Albumin (g/dL)3.85±0.053.69±0.073.60±0.063.26±0.133.12±0.23
Prothrombin time (seconds)11.19±0.1411.50±0.1512.10±0.5814.00±0.6312.60±0.81
AFP (ng/mL)5.86±0.458.87±0.657.00±1.0525.63±8.9430.20±12.04
Fibrosis score (1-6)—1.93±0.232.00±0.324.50±0.194.40±0.24
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