1. bookVolume 16 (2016): Issue 4 (October 2016)
Journal Details
First Published
25 Nov 2011
Publication timeframe
4 times per year
access type Open Access

The Effect of Adding Hesperidin, Diosmin, Quercetin and Resveratrol Extracts to Feed for Turkey Hens on Selected Immunological and Biochemical Blood Indices

Published Online: 27 Oct 2016
Volume & Issue: Volume 16 (2016) - Issue 4 (October 2016)
Page range: 1101 - 1114
Received: 23 Feb 2016
Accepted: 09 May 2016
Journal Details
First Published
25 Nov 2011
Publication timeframe
4 times per year

It was postulated that naturally occurring phenolic compounds obtained from various plant species may have potential use as feed additives for poultry. Therefore the aim of the study was to compare extracts of hesperidin, diosmin, quercetin and resveratrol in terms of their health-promoting (particularly immunostimulatory) effect on turkeys at different ages. The experiment was conducted on 720 Big 6 turkey hens assigned to 6 experimental groups of 120 individuals (6 repetitions with 20 birds each). The turkey hens in group G-C were the control, receiving a basal compound feed with no experimental additives. The turkey hens in the remaining groups, from the first to the 16th week of life, received a basal diet containing hesperidin (group G-H), diosmin (group G-D), quercetin (group G-Q) or resveratrol (group G-R) in the amount of 200 g per tonne of feed. Ht, Hb, RBC, WBC, lysozyme activity, %PC, IgA, IL-6, GLU, TP and minerals were determined in blood samples. The addition of quercetin or resveratrol in the amount of 200 g per tonne of feed was found to have a beneficial effect on haemoglobin synthesis and phosphorus availability, and may also modulate immunity in turkey hens.


Abdulkarimi R., Daneshyar M. (2012). The effects of thyme (Thymus vulgaris) extract supplementation in drinking water on iron metabolism in broiler chickens. J. Med. Plants Res., 6: 645-650.Search in Google Scholar

Adamu M., Boonkaewwan C., Gongruttananun N., Vongpakorn M. (2013). Hematological, biochemical and histopathological changes caused by coccidiosis in chickens. Kasetsart J. (Nat. Sci.), 47: 238-246.Search in Google Scholar

Addass P.A., David D.L., Edward A., Zira K.E., Midau A. (2012). Effect of age, sex and management system on some haematological parameters of intensively and semi-intensively kept chicken in Mubi, Adamawa State, Nigeria. IJAS, 2: 277-282.Search in Google Scholar

Alagawany M., Abd El- Hack M.E. (2015). The effect of rosemary herb asadietary supplement on performance, egg quality, serum biochemical parameters and oxidative status in laying hens. J. Anim. Feed Sci., 24: 341-347.Search in Google Scholar

Arczewska-Włosek A., Świątkiewicz S. (2015). The efficacy of selected feed additives in the prevention of broiler chicken coccidiosis under natural exposure to Eimeria spp. Ann. Anim. Sci., 15: 725-735.Search in Google Scholar

Bomski J. (1989). Basic Laboratory Hematological Analyses (in Polish). Warsaw, Poland, PZWL Search in Google Scholar

Chen Y.T., Zheng R.L., Jia Z.J., Ju Y. (1990). Flavonoids as superoxide scavengers and antioxidants. Free Radic. Biol. Med., 9: 19-21.Search in Google Scholar

Christev C., Nickolova M., Penkov D., Ivanova R., Abadjieva D., Grigorova S. (2011). Investigation of the effect of Tribulus terrestris extract on the main biochemical and haematological indices of the blood in guinea fowls (Numida meleagris). J. Cent. Eur. Agr., 12: 16-26.Search in Google Scholar

Dougnon T.J., Kiki P., Dougnon T.V., Youssao I. (2014). Evaluation of Capsicum frutescens powder effects on the growth performances, biochemical and hematological parameters in Hubbard broiler. J. App. Pharm. Sci., 4: 38-43.Search in Google Scholar

Feldman B., Zinkl J., Jain N. (2000). Schalm’s Veterinary Haematology. Baltimore, USA, Lippincott Williams and Wilkins. 5th ed., 1376 pp.Search in Google Scholar

Frémont L., Belguendouz L., Delpal S. (1999). Antioxidant activity of resveratrol and alcohol- free wine polyphenols related to LDLoxidation and polyunsaturated fatty acids. Life Sci., 64: 2511-2521.Search in Google Scholar

Galati E.M., Monforte M.T., Kirjavainen S. (1994). Biological effects of hesperidin,acitrus flavonoid: anti-inflammatory and analgesic activity. Farmaco., 40: 709-712.Search in Google Scholar

Gheith I.M., Fararh K.M., Bakry H.H., Hosney G.A. (2011). Clinicopathological studies on the effect of enteric diseases in broiler chicks. Banha Vet. Med. J., 22: 17-26.Search in Google Scholar

Grajek W. (2007). Antioxidants in food (in Polish). Warsaw, Poland, WNT.Search in Google Scholar

Grela E.R., Ognik K., Czech A., Matras J. (2014). Quality assessment of eggs from laying hens fedamixture with lucerne protein concentrate. J. Anim. Feed Sci., 23: 236-243.Search in Google Scholar

Hager- Theodorides A.L., Goliomytis M., Delis S., Dligeorgis S. (2014). Effects of dietary supplementation with quercetin on broiler immunological characteristics. Anim. Feed Sci. Tech., 198: 224-230.Search in Google Scholar

Jaccob A.A., Hussain S.A. (2012). Effects of long-term use of flavonoids on the absorption and tissue distribution of orally administered doses of trace elements in rats. Pharmacology & Pharmacy, 3: 474-480.Search in Google Scholar

Jankowski J., Juśkiewicz J., Zduńczyk P., Kosmala M., Zieliński H., Antoszkiewicz Z., Zduńczyk Z. (2016). Antioxidant status of blood and liver of turkeys fed diets enriched with polyunsaturated fatty acids and fruit pomaces asasource of polyphenols. Pol. J. Vet. Sci., 19: 89-98.Search in Google Scholar

John C.M., Sandrasaigaran P., Tong C.K., Adam A., Ramasamy R. (2011). Immunomodulatory activity of polyphenols derived from Cassia auriculata flowers in aged rats. Cell Immunol., 271: 474-479.Search in Google Scholar

Juśkiewicz J., Jankowski J., Zduńczyk Z., Kołodziejczyk K., Mikulski D., Zduńczyk P. (2015). The chemical composition of selected dried fruit pomaces and their effects on the growth performance and post-slaughter parameters of young turkeys. J. Anim. Feed Sci., 24: 53-60.Search in Google Scholar

Kang O.H., Jang H.J., Chae H.S., Oh Y.C., Choi J.G., Lee Y.S., Kim J.H., Kim Y.C., Sohn D.H., Park H., Kwon D.Y. (2009). Anti-inflammatory mechanisms of resveratrol in activated HMC-1 cells: Pivotal roles of NF-κBand MAPK. Pharmacol. Res., 59: 330-337.Search in Google Scholar

Kasapis C., Thompson P.D. (2005). The effects of physical activity on serum C-reactive protein and inflammatory markers:asystematic review. J. Am. Coll. Cardiol., 45: 1563-1569.Search in Google Scholar

Kehinde A.S., Obun C.O., Inuwa M., Bobadoye O. (2011). Growth performance, haematological and serum biochemical indices of cockerel chicks fed ginger (Zingiber officinale) additive in diets. Anim. Res. Int., 8: 1398-1404.Search in Google Scholar

Kim E.T., Guanle L., Lee S.J., Lee S.M., Lee S.S., Lee I.D., Lee S.K., Lee S.S. (2015). Effects of flavonoid-rich plant extracts on in vitro ruminal methanogenesis, microbial populations and fermentation characteristics. Asian-Australas. J. Anim. Sci., 28: 530-537.Search in Google Scholar

Koncicki A., Krasnodębska - Depta A. (2005). The possibility of using hematology and biochemistry in the diagnosis of poultry diseases (in Polish). Mag. Wet. Supl. Drób, pp. 20-22.Search in Google Scholar

Kongkathip N., Teerawattanowanich C., Chantakru S., Kongkathip B., Song- serm T., Pankaew Y., Isariyodam S. (2010). Broiler ration plus Curcuma longa extracts for protection against diseases-causing viruses. Virus and target cell interaction inhibition. Patent International Application (2010). CODEN PIXCDZ WO 2010062260 71 20100603 CAN 153:21014:683175 CAPLUS. 24 pp.Search in Google Scholar

Kwiecień M., Winiarska- Mieczan A., Pałyszka M., Kwiatkowska K. (2014). The effects of chicken feed herbal additives on the breeding performance, slaughter yield and mechanical and morphometric parameters of chicken tibia bones. Med. Weter., 70: 280-286.Search in Google Scholar

Lloyd S., Gibson J.S. (2006). Haematology and biochemistry in healthy young pheasants and redlegged partridges and effects of spironucleosis on these parameters. Avian Pathol., 35: 335-340.Search in Google Scholar

Min Y.D., Choi C .H., Bark H ., Son H .Y., Park H .H., Lee S ., Park J .W., Park E .K., Shin H.I., Kim S.H. (2007). Quercetin inhibits expression of inflammatory cytokines through attenuation of NF-kappa Band p38 MAPKin HMC-1 human mast cell line. Inflamm. Res., 56: 210-215.Search in Google Scholar

Nutrient Requirements for Poultry (2005). A. Rutkowski (Co-editor). Collective work. Jabłonna, Poland, PAN IFi ZZ, 4th ed. revised and enlarged.Search in Google Scholar

Ognik K. (2013). The possibility of using natural antioxidants in feeding of slaughter poultry as inhibitors of lipid peroxidation (in Polish). In: Rozprawy Naukowe Uniwersytetu Przyrodniczego w Lublinie 370, Lublin, Poland, 146 pp.Search in Google Scholar

Ognik K., Czech A. (2010). The effect of diversified doses of aloes plus plant preparation on the level of antioxidant indices in the blood of turkey hens. J. Appl. Anim. Res., 38: 45-48.Search in Google Scholar

Ognik K., Czech A. (2014). Effect of applying soybean and linseed oil and different forms of tocopherol on the redox and immune profiles in blood of slaughter turkey hens. South Afr. J. Anim. Sci., 44: 322-334.Search in Google Scholar

Ognik K., Sembratowicz I. (2012). Effect of Aloe plus preparation supplement on hematological and immunological blood parameters of and performance of turkey hens. Turkish J. Vet. Anim. Sci., 36: 491-498.Search in Google Scholar

Ognik K., Czech A., Stachyra K. (2013). Effect ofanatural versusasynthetic antioxidant, and sex and age on the redox profile in the blood of growing turkeys. South Afr. J. Anim. Sci., 43: 473-481.Search in Google Scholar

Ognik K., Sembratowicz I., Czech A., Kulak E., Merska M. (2015 a). Effect of an aloe preparation and 5-oxo-1,2,4-triazine on the redox profile of the blood of turkey hens subjected to stress. Ann. Anim. Sci., 15: 93-105.10.2478/aoas-2014-0079Search in Google Scholar

Ognik K., Sembratowicz I., Czech A. (2015 b). Effect of an aloe preparation and 5-oxo-1,2,4- triazine on immunological and haematological parameters of the blood of turkey hens subjected to stress. Acta Vet. Brno, 4: 365-371.10.2754/avb201584040365Search in Google Scholar

Ognik K., Cholewińska E., Sembratowicz I., Grela E.R., Czech A. (2016). The potential of using plant antioxidants to stimulate antioxidant mechanisms in poultry. World Poultry Sci. J., 72: 1-8.Search in Google Scholar

Omar H.R., Komarova I., El- Ghonemi M., Fathy A., Rashad R., Abdelmalak H.D., Yerramadha M.R., Ali Y., Helal E., Camporesi E.M. (2012). Licorice abuse: time to sendawarning message. Ther. Adv. Endocrinol. Metab., 3: 125-138.Search in Google Scholar

Park B.H., Fikrig S.M., Smithuick E.M. (1968). Infection and nitroblue tetrazolium reduction by neutrophils. Lancet, 2: 532.Search in Google Scholar

Pereira Lima G.P.P., Vianello F., Corrêa C.R., Arnouxda Silva Campos R., Galhardo Borguini M. (2014). Polyphenols in fruits and vegetables and its effect on human health. J. Nutr. Food Sci., 5: 1065-1082.Search in Google Scholar

Petiwala S.M., Puthenveetil A.G., Johnson J.J. (2013). Polyphenols from the Mediterranean herb rosemary (Rosmarinus officinalis) for prostate cancer. Front Pharmacol., 4: 29.Search in Google Scholar

Pietta P.G. (2000). Flavonoids as antioxidants. J. Nat. Prod., 63: 1035-1042.Search in Google Scholar

Pinkiewicz E. (1971). Laboratory Diagnostics of Animal Diseases. Higher School Agriculture, Lublin, Poland, pp. 481-482.Search in Google Scholar

Pourhossein Z., Qotbi A.A.A., Seidavi A., Laudadio V., Centoducati G., Tu- farelli V. (2015). Effect of different levels of dietary sweet orange (Citrus sinensis) peel extract on humoral immune system responses in broiler chickens. Anim. Sci. J., 86: 105-110.Search in Google Scholar

Rafiee A., Rahimian Y., Zamani F., Asgarian F. (2013). Effect of use ginger (Zingiber officinale) and thymus (Thymus vulgaris) extract on performance and some hematological parameters on broiler chicks. Sci. Agri., 4: 20-25.Search in Google Scholar

Rasouli E., Jahanian R., (2015). Improved performance and immunological responses as the result of dietary genistein supplementation of broiler chicks. Animal, 9: 1473-1480.Search in Google Scholar

Rice-Evans C.A., Miller N.J., Paganga G. (1996). Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Rad. Biol. Med., 20: 933-956.Search in Google Scholar

Rusinek-Prystupa E., Tatara M.R. (2014). Effect ofaplant preparation Citrosept on selected immunity indices in blood of slaughter turkey hens. Ann. Agric. Environ. Med., 21: 581-584.Search in Google Scholar

Sedghi M., Golian A., Kermanshahi H., Ahmadi H. (2010). Effect of dietary supplementation of licorice extract andaprebiotic on performance and blood metabolites of broilers. S. Afr. J. Anim. Sci., 40: 371-380.Search in Google Scholar

Sezer A., Usta U., Kocak Z., Yagci M.A. (2011). The effect ofaflavonoid fractions diosmin + hesperidin on radiation-induced acute proctitis inarat model. J. Cancer Res. Ther., 7: 152-156.Search in Google Scholar

Siwicki A.K., Anderson D.P. (1993). Immunostimulation in fish: Measuring the effects of stimulants by serological and immunological methods. U.S. Fish and Wildlife Service Inland Fisheries Institute in Olsztyn, pp. 321-326.Search in Google Scholar

Smulikowska S., Rutkowski A. (2005). Nutrient requirements of poultry (in Polish). Jablonna, Poland, The Kielanowski Institute of Animal Physiology and Nutrition PAS, 4th ed.Search in Google Scholar

Song N.R., Kim J.E., Park J.S., Kim J.R., Kang H., Lee E., Kang Y.G., Son J.E., Seo S.G., Heo Y.S., Lee K.W. (2015). Licochalcone A,apolyphenol present in licorice, suppresses UVinduced COX-2 expression by targeting PI3K, MEK1, and B-Raf. Int. J. Mol. Sci., 16: 4453-4470.Search in Google Scholar

Tako E., Beebe S.E., Reed S., Hart J.J., Glahn R.P. (2014). Polyphenolic compounds appear to limit the nutritional benefit of biofortified higher iron black bean (Phaseolus vulgaris L.). J. Nutr., 13: 28.Search in Google Scholar

Talebi A., Asri- Rezaei S., Rozeh- Chai R., Sahrae R. (2005). Comparative studies on haematological values of broiler strains (Ross, Cobb, Arbor-acres and Arian). Int. J. Poult. Sci., 4: 573-579.Search in Google Scholar

Unigwe C.R. (2011). Effect of graded levels of Hibiscus sabdariffa Linn. (Rosella) calyx extract on growth performance and haematology of broiler chickens. Glob. J. Sci. Res., 1: 78-81.Search in Google Scholar

Vauzour D., Rodriguez- Mateos A., Corona G., Oruna- Concha M.J., Spen- cer J.P.E. (2010). Polyphenols and human health: prevention of disease and mechanisms of action. Nutrients, 2: 1106-1131.Search in Google Scholar

Wung B.S., Hsu M.C., Wu C.C., Hsieh C.W. (2005). Resveratrol suppresses IL-6-induced ICAM-1 gene expression in endothelial cells: Effects on the inhibition of STAT3 phosphorylation. Life Sci., 4: 389-397.Search in Google Scholar

Zimmer A.R., Leonardi B., Miron D., Schapoval E., Oliveira J.R., Gosmann G. (2012). Antioxidant and anti-inflammatory properties of Capsicum baccatum: From traditional use to scientific approach. J. Ethnopharmacol., 139: 228-233. Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo