1. bookVolume 67 (2017): Issue 3 (September 2017)
Journal Details
License
Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
access type Open Access

Effects of Selenium and Thyroid Hormone Deficiency on Peritoneal Macrophages Adhesion and Occurrence of Natural IGM Antibodies in Juvenile Rats

Published Online: 23 Sep 2017
Volume & Issue: Volume 67 (2017) - Issue 3 (September 2017)
Page range: 340 - 355
Received: 07 Mar 2017
Accepted: 20 Jun 2017
Journal Details
License
Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
Abstract

Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual influence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium deficiency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats. The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU- group; selenium adequate, PTU+ group; selenium defi cient, PTU- group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 mg/L) in drinking water. After 3 weeks, thyroxine (T4), triiodothyronine (T3), and thyroid stimulating hormone (TSH) were determined. The animals having “intermediate” concentrations of T3 (1.56-1.69 nmol/L) and T4 (41-50 nmol/L) were excluded from further analysis. Thus, PTU+ groups included hypothyroid animals (T3≤1.55 nmol/L; T4≤40 nmol/L), while PTU- groups included euthyroid rats (T3≥1.70 nmol/L; T4≥50 nmol/L). Both groups of selenium deficient rats had, when compared to the control group, a significantly lower activity of glutathione peroxidase GPx1 and GPx3. Neither selenium deficiency nor PTU influenced the adherence of peritoneal macrophages. Selenium deficiency significantly decreased the peroxide synthesis in macrophages and significantly increased the titer of anti-SRBC IgM. Hypotyroidism alone or in combination with selenium deficiency had no influence on these parameters.

Keywords

1. McKenzie RC, Arthur JR, Miller SM, Rafferty TS, Beckett GJ: Selenium and the immune system. In: Nutrition and Immune Function, CAB International, Oxford, U.K 2002, 229-250.10.1079/9780851995830.0229Search in Google Scholar

2. Arthur JR, Nicol F, Beckett GJ: Hepatic iodothyronine 5’ deiodinase. The role of selenium. Biochem J 1990, 272:537-540.10.1042/bj272053711497352268281Search in Google Scholar

3. Hoffmann PR, Hoge SC, Li PA, Hoffman FV, Hashimoto AC, Berry MJ: The selenoproteome exhibits widely varying, tissue-specific dependence on selenoprotein P for selenium supply, Nucleic Acids Res 2007, 35:3963-3973.10.1093/nar/gkm355191948917553827Search in Google Scholar

4. Berry MJ, Banu L, Chen YY, Mandel SJ, Kieffer JD, Harney JW, Larsen PR. Recognition of UGA as a selenocysteine codon in type I deiodinase requires sequnces in the 3’ untransllated region, Nature 1991, 353, 273-27610.1038/353273a01832744Search in Google Scholar

5. Tarp U, Overvad K, Hansen JC, Thorling EB: Low selenium level in severe rheumatoid arthritis. Scand J Rheumatol 1985, 14:97-101.10.3109/030097485091654904001893Search in Google Scholar

6. Toulis KA, Anastasilakis AD, Tzellos TG, Goulis DG, Kouvelas D: Selenium supplementation in the treatment of Hashimoto’s thyroiditis: a systematic review and a meta-analysis. Thyroid 2010, 20:1163-1173.10.1089/thy.2009.035120883174Search in Google Scholar

7. Turner RJ, Finch JM: Selenium and the immune response. Proc Nutr Soc 1991. 50: 275-285.10.1079/PNS199100371749795Search in Google Scholar

8. Baker SS, Cohen HJ: Altered oxidative metabolism in selenium-deficient rat granulocytes. J Immunol 1983, 130(6):2856-2860Search in Google Scholar

9. Kim SH, Johnson VJ, Shin TY, Sharma RP: Selenium attenuates lipopolysaccharide-induced oxidative stress responses through modulation of p38 MAPK and NF-kappaB signaling pathways. Exp Biol Med (Maywood) 2004, 229:203-13.10.1177/15353702042290020914734799Search in Google Scholar

10. Huang Z, Aaron A, Hoffmann P: The role of selenium in inflammation and immunity: from molecular mechanisms to therapeutic opportunities. Antioxid Redox Signal 2012, 16:705-743.10.1089/ars.2011.4145327792821955027Search in Google Scholar

11. Kiremidjian-Schumacher L, Roy M, Wishe HI, Cohen MW, Stotzky G: Supplementation with selenium and human immune cell functions. II. Effect on cytotoxic lymphocytes and natural killer cells. Biol Trace Elem Res 1994, 41:115-27.10.1007/BF029172227946899Search in Google Scholar

12. Kiremidjian-Schumacher L, Roy M, Wishe HI, Cohen MW, Stotzky G: Regulation of cellular immune responses by selenium. Biol Trace Elem Res 1992, 33:23-35.10.1007/BF02783989Search in Google Scholar

13. Kiremidjian-Schumacher L, Roy M, Wishe HI, Cohen MW, Stotzky G, Selenium and immune cell functions. I. Effect on lymphocyte proliferation and production of interleukin 1 and interleukin 2. Proc Soc Exp Biol Med 1990, 193:136-42.10.3181/00379727-193-43014Search in Google Scholar

14. Roy M, Kiremidjian-Schumacher L, Wishe HI, Cohen MW, Stotzky G: Supplementation with selenium and human immune cell functions. I. Effect on lymphocyte proliferation and interleukin 2 receptor expression. Biol Trace Elem Res 1994, 41:103-14.10.1007/BF02917221Search in Google Scholar

15. De Vito P, Incerpi S, Pedersen JZ, Luly P, Davis FB, Davis PJ: Thyroid hormones as modulators of immune activities at the cellular level. Thyroid 2011, 21(8):879-890.10.1089/thy.2010.0429Search in Google Scholar

16. Rosa LF, Safi DA, Curi R: Effect of hypo- and hyperthyroidism on the function and metabolism of macrophages in rats. Cell Biochem Funct 1995, 13:141-7.10.1002/cbf.290130211Search in Google Scholar

17. Klecha AJ, Genaro AM, Lysionek AE, Caro RA, Coluccia AG, Cremaschi GA: Experimental evidence pointing to the bidirectional interaction between the immune system and the thyroid axis. Int J Immunopharmacol 2000, 22:491-50010.1016/S0192-0561(00)00012-6Search in Google Scholar

18. Klecha AJ, Genaro AM, Gorelik G, Barreiro Arcos ML, Silberman DM, Schuman M, Garcia SI, Pirola C, Cremaschi GA: Integrative study of hypothalamus-pituitary-thyroidimmune system interaction: thyroid hormone-mediated modulation of lymphocyte activity through the protein kinase C signaling pathway. J Endocrinol. 2006, 189:45-55.10.1677/joe.1.06137Search in Google Scholar

19. Behne D, Kyriakopoulos A, Meinhold H, Köhrle J, 1990, Identifi cation of type I iodothyronine 5’-deiodinase as a selenoenzyme, Biochem Biophys Res Commun, 31, 173(3), 1143-9.10.1016/S0006-291X(05)80905-2Search in Google Scholar

20. Arthur JR, McKenzie RC, Beckett GJ: Selenium in the immune system. J Nutr 2003, 133:1457-9.10.1093/jn/133.5.1457S12730442Search in Google Scholar

21. Abbas AK, Lichtman AH, Pillai S: Cellular and Molecular Immunology (Seventh edition) 2012, Elsevier/ Saunders, Philadelphia, PA, USA.Search in Google Scholar

22. Day MJ, Schultz RD. Veterinary Immunology: Principles and Practice. 2011. Manson publishing/The veterinary press, London, UK.10.1201/b15224Search in Google Scholar

23. Callahan GN, Yates RM. Basic Veterinary Immunology. 2014. University Press of Colorado, Boulder, Colorado.Search in Google Scholar

24. Boes M: Role of natural and immune IgM antibodies in immune responses. Mol Immunol 2000, 37(18):1141-1149.10.1016/S0161-5890(01)00025-6Search in Google Scholar

25. Grönwall C, Vas J, Silverman GJ: Protective Roles of Natural IgM Antibodies. Front Immunol 2012, 3:66.10.3389/fimmu.2012.00066Search in Google Scholar

26. Lobo PI: Role of natural autoantibodies and natural IgM anti-leucocyte autoantibodies in health and disease. Front Immunol 2016, 7:198.10.3389/fimmu.2016.00198Search in Google Scholar

27. Günzler WA, Steffens GJ, Grossman A, Kim SMA, Otting F, Wendel A, Flohe L: The aminoacid sequence of a bovine glutathione peroxidase. Hoppe-Seyler’s Z Physiol Chem 1974, 365:19510.1515/bchm2.1984.365.1.195Search in Google Scholar

28. Sankari: Plasma glutathione peroxidase and tissue selenium response to selenium supplementation in swine. Acta Vet. Scand 1985, 81 (Suppl.):1-127.Search in Google Scholar

29. Oez S, Welte K, Platzer E, Kalden JR: A simple assay for quantifying the inducible adherence of neutrophils. Immunobiology 1990, 180:308-315.10.1016/S0171-2985(11)80294-9Search in Google Scholar

30. Pick E, Mizel D: Rapid microassays for the measurement of superoxide and hydrogen peroxide production by macrophages in culture using an automatic enzyme immunoassay reader. Journal of Immunological Methods 1981, 46:211-226.10.1016/0022-1759(81)90138-1Search in Google Scholar

31. Sunde RA: Regulation of glutathione peroxidase-1 expression. In: Hatfi eld DL, Berry MJ, Gladyshev VN, editors. Selenium: its molecular biology and role in human health. 2nd ed. New York: Springer Science Media 2006, 149-60.10.1007/0-387-33827-6_14Search in Google Scholar

32. Hatfield DL, Selenium: Its molecular biology and role in human health, Kluver academic publishers 2001,10.1007/978-1-4615-1609-5Search in Google Scholar

33. Hill K, Burk R, Lane J: Effect of selenium depletion and repletion on plasma glutathione and glutathione dependent enzymes in rat. J Nutr 1987, 117:99-104.10.1093/jn/117.1.993819879Search in Google Scholar

34. Cettour-Rose P, Visser1 TJ, Burger AG, Rohner-Jeanrenaud F: Inhibition of pituitary type 2 deiodinase by reverse triiodothyronine does not alter thyroxine-induced inhibition of thyrotropin secretion in hypothyroid rats. Eur J Endocrinol 2005, 153:429-434.10.1530/eje.1.0198416131606Search in Google Scholar

35. Ferreira E, Silva AE, Serakides R, Gomes AES, Cassali GD: Model of induction of thyroid dysfunctions in adult female mice. Arq Bras Med Vet Zootec 2007, 59:1245-1249.10.1590/S0102-09352007000500022Search in Google Scholar

36. Kabanda L, Lefebvre RA, Van Bree HJ, Remon JP: In vitro and in vivo evaluation in dogs and pigs of a hydrophilic matrix containing propylthiouracil, Pharm Res 1994, 11:1663-1668.10.1023/A:1018982409661Search in Google Scholar

37. Veronikis IE, Braverman LE, Alex S, Fang SL, Norvell B, Emerson CH: Comparison of the effects of propylthiouracil and selenium defi ciency on T3 production in the rat. Endocrinology 1996, 137:2580-2585.10.1210/endo.137.6.8641212Search in Google Scholar

38. Bianco A , Kim B: Deiodinases: Implications of the local control of thyroid hormone action, J Clin Invest 2006, 116:2571-2579.10.1172/JCI29812Search in Google Scholar

39. Eichner RD, Smeaton TC: Agar accumulates in rat peritoneal macrophages elicited with thioglycollate broth. Scand J Immunol 1983, 18:259-26310.1111/j.1365-3083.1983.tb00866.xSearch in Google Scholar

40. Melnicoff MJ, Horan PK, Morahan PS: Kinetics of changes in peritoneal cell populations following acute infl ammation. Cell Immunol 1989, 118:178.10.1016/0008-8749(89)90367-5Search in Google Scholar

41. Tak PP, Firestein GS: NF-κB: a key role in infl ammatory diseases. J Clin Invest 2001, 107:7-11.10.1172/JCI1183019855211134171Search in Google Scholar

42. Marazuela M, Postigo AA, Acevedo A, Díaz-González F, Sanchez-Madrid F, de Landázuri MO : Adhesion molecules from the LFA-1/ICAM-1,3 and VLA-4/VCAM-1 pathways on T lymphocytes and vascular endothelium in Graves’ and Hashimoto’s thyroid glands. Eur J Immunol 1994, 24:2483-90.10.1002/eji.18302410347523142Search in Google Scholar

43. Wenisch C, Myskiw D, Gessl A, Graninger W: Circulating selectins, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1 in hyperthyroidism. J Clin Endocrinol Metab 1995, 80:2122-2126.10.1210/jcem.80.7.75418027541802Search in Google Scholar

44. Cho KJ, Seo JM, Kim JH: Bioactive Lipoxygenase Metabolites Stimulation of NADPH Oxidases and Reactive Oxygen Species. Mol Cells 2011, 32(1):1-5.10.1007/s10059-011-1021-7388765621424583Search in Google Scholar

45. Duntas LH: Selenium and Infl ammation: Underlying anti-infl ammatory mechanisms. Horm Metab Res 2009, 41:443-447.10.1055/s-0029-122072419418416Search in Google Scholar

46. Vunta H, Davis F, Palempalli U, Bhat D, Arner R, Thompson J, Peterson D, Reddy C, Prabhu KS: The Anti-infl ammatory Effects of Selenium Are Mediated through 15-Deoxy-Δ12,14-prostaglandin J2 in Macrophages. J Biol Chem 2007, 282:17964-17973.10.1074/jbc.M70307520017439952Search in Google Scholar

47. Ladics GS: Primary immune response to sheep red blood cells (SRBC) as the conventional T-Cell dependent antibody response (TDAR) test. J Immunotox 2007, 4:149-152.10.1080/1547691070133735718958723Search in Google Scholar

48. Witkowska AM, Kuryliszyn-Moskal A, Borawska MH, Hukałowicz K, Markiewicz R: A study on soluble intercellular adhesion molecule-1 and selenium in patients with rheumatoid arthritis complicated by vasculitis. Clin Rheumatol 2003, 22:414-419.10.1007/s10067-003-0799-x14677018Search in Google Scholar

49. Duntas LH: The Role of Iodine and Selenium in Autoimmune Thyroiditis. Horm Metab Res 2015, 47(10):721-726.10.1055/s-0035-155963126361258Search in Google Scholar

50. Li J, Liang Y, Mao H, Deng W, Zhang J: Effects of B-lymphocyte dysfunction on the serum copper, selenium and zinc levels of rheumatoid arthritis patients. Pak J Med Sci 2014, 30:1064-1067.10.12669/pjms.305.5214416323325225527Search in Google Scholar

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