<abstract xmlns="http://www.w3.org/1999/xhtml"> Silicon-substituted calcium phosphate (Si-CaP) was developed in our laboratory as a biomaterial for delivery in bone tissue engineering. It was fabricated as a 3D-construct of scaffolds using chitosan-trisodium polyphosphate (TPP) cross-linked networks. In this study, heparin was covalently bonded to the residual -NH2 groups of chitosan on the scaffold applying carbodiimide chemistry. Bonded heparin was not leached away from scaffold surfaces upon vigorous washing or extended storage. Recombinant human bone morphogenetic protein 2 (rhBMP-2) was bound to conjugated scaffolds by ionic interactions between the negatively charged SO42- clusters of heparin and positively charged amino acids of rhBMP-2. The resulting scaffolds were inspected for bone regenerative capacity by subcutaneous implanting in rats. Histological observation and mineralization assay were performed after 4 weeks of implantation. Results from both in vitro and in vivo experiments suggest the potential of the developed scaffolds for bone tissue engineering applications in the future.</abstract>

Silicon-substituted calcium phosphate (Si-CaP) was developed in our laboratory as a biomaterial for delivery in bone tissue engineering. It was fabricated as a 3D-construct of scaffolds using chitosan-trisodium polyphosphate (TPP) cross-linked networks. In this study, heparin was covalently bonded to the residual -NH2 groups of chitosan on the scaffold applying carbodiimide chemistry. Bonded heparin was not leached away from scaffold surfaces upon vigorous washing or extended storage. Recombinant human bone morphogenetic protein 2 (rhBMP-2) was bound to conjugated scaffolds by ionic interactions between the negatively charged SO42- clusters of heparin and positively charged amino acids of rhBMP-2. The resulting scaffolds were inspected for bone regenerative capacity by subcutaneous implanting in rats. Histological observation and mineralization assay were performed after 4 weeks of implantation. Results from both in vitro and in vivo experiments suggest the potential of the developed scaffolds for bone tissue engineering applications in the future.

A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2

Department of Pharmaceutical Chemistry Faculty of Pharmaceutical Sciences Prince of Songkla University Hat-yai, Songkhla, Thailand

Department of Pharmaceutical Chemistry Faculty of Pharmaceutical Sciences and Nanotec-PSU Center of Excellence on Drug Delivery System Prince of Songkla University Hat-yai, Songkhla, Thailand

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Silicon-substituted calcium phosphate (Si-CaP) was developed in our laboratory as a biomaterial for delivery in bone tissue engineering. It was fabricated as a...

A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2

Published Online: Jun 29, 2016

Page range: 373 - 385

Accepted: Mar 01, 2016

DOI: https://doi.org/10.1515/acph-2016-0026

Keywords
heparin, carbodiimide, rhBMP-2, delivery scaffold, bone tissue engineering

© by Jasadee Kaewsrichan

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2

Published Online: Jun 29, 2016

Page range: 373 - 385

Accepted: Mar 01, 2016

DOI: https://doi.org/10.1515/acph-2016-0026

Keywordsheparin, carbodiimide, rhBMP-2, delivery scaffold, bone tissue engineering

© by Jasadee Kaewsrichan

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
heparin, carbodiimide, rhBMP-2, delivery scaffold, bone tissue engineering