<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The aim of our study was to identify the most suitable flotation solution and effective preservation method for the examination of equine faeces samples using the FLOTAC technique. Samples from naturally infected horses were transported to the laboratory and analysed accordingly. The sample from each horse was homogenized and divided into four parts: one was frozen, another two were preserved in different reagents such as sodium acetate-acetic-acid-formalin(SAF) or 5% formalin. The last part was examined as a fresh sample in three different flotation solutions (Sheather´s solution, sodium chloride and sodium nitrate solution, all with a specific gravity 1.200). The preserved samples were examined in the period from 14 to 21 days after collection. According to our results, the sucrose solution was the most suitable flotation solution for fresh samples (small strongyle egg per gram was 706 compared to 360 in sodium chlorid and 507 in sodium nitrate) and the sodium nitrate solution was the most efficient for the preserved samples (egg per gram was 382 compared to 295 in salt solution and 305 in sucrose solution). Freezing appears to be the most effective method of sample preservation, resulting in minimal damage to fragile strongyle eggs and therefore it is the most simple and effective preservation method for the examination of large numbers of faecal samples without the necessity of examining them all within 48 hours of collection. Deep freezing as a preservation method for equine faeces samples has not, according to our knowledge, been yet published.</p></abstract>

The aim of our study was to identify the most suitable flotation solution and effective preservation method for the examination of equine faeces samples using the FLOTAC technique. Samples from naturally infected horses were transported to the laboratory and analysed accordingly. The sample from each horse was homogenized and divided into four parts: one was frozen, another two were preserved in different reagents such as sodium acetate-acetic-acid-formalin(SAF) or 5% formalin. The last part was examined as a fresh sample in three different flotation solutions (Sheather´s solution, sodium chloride and sodium nitrate solution, all with a specific gravity 1.200). The preserved samples were examined in the period from 14 to 21 days after collection. According to our results, the sucrose solution was the most suitable flotation solution for fresh samples (small strongyle egg per gram was 706 compared to 360 in sodium chlorid and 507 in sodium nitrate) and the sodium nitrate solution was the most efficient for the preserved samples (egg per gram was 382 compared to 295 in salt solution and 305 in sucrose solution). Freezing appears to be the most effective method of sample preservation, resulting in minimal damage to fragile strongyle eggs and therefore it is the most simple and effective preservation method for the examination of large numbers of faecal samples without the necessity of examining them all within 48 hours of collection. Deep freezing as a preservation method for equine faeces samples has not, according to our knowledge, been yet published.

Original Scientific Article. Assessment of Reagent Effectiveness and Preservation Methods for Equine Faecal Samples

University of Veterinary and Pharmaceutical Sciences, Faculty of Veterinary Medicine, Equine Clinic, Brno, Czech Republic

Equine Clinic, Faculty of Veterinary Medicine University of Veterinary and Pharmaceutical Sciences Brno Palackého 1/3, 612 42 Brno, Czech Republic

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The aim of our study was to identify the most suitable flotation solution and effective preservation method for the examination of equine faeces samples using...

Original Scientific Article. Assessment of Reagent Effectiveness and Preservation Methods for Equine Faecal Samples

Published Online: Nov 18, 2015

Page range: 101 - 106

Received: Oct 09, 2014

Accepted: Jan 22, 2015

DOI: https://doi.org/10.14432/j.macvetrev.2015.02.040

Keywords
horse, flotation solution, small strongyles, freezing, formalin

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Original Scientific Article. Assessment of Reagent Effectiveness and Preservation Methods for Equine Faecal Samples

Published Online: Nov 18, 2015

Page range: 101 - 106

Received: Oct 09, 2014

Accepted: Jan 22, 2015

DOI: https://doi.org/10.14432/j.macvetrev.2015.02.040

Keywordshorse, flotation solution, small strongyles, freezing, formalin

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
horse, flotation solution, small strongyles, freezing, formalin