<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Staphylococcus aureus is considered as one of the most important and common pathogens of bovine mastitis. Polymerase Chain Reaction is frequently proposed in the diagnosis of S. aureus directly from milk samples instead of classical culture. However, false-negative results may occur in the polymerase chain reaction analysis performed directly from clinical material. For the purpose of disclosing the false negative results, the use of internal amplification controls can be beneficial. Therefore, in this study a new polymerase chain reaction technique with host specific internal amplification control was developed by optimizing S. aureus-specific primers in combination with bovine specific primers. The effectiveness of the developed technique in this study was attempted in milk samples from bovine subclinical mastitis. This technique has the potential to detect S. aureus from bovine milk samples or dairy products.</p></abstract>

Staphylococcus aureus is considered as one of the most important and common pathogens of bovine mastitis. Polymerase Chain Reaction is frequently proposed in the diagnosis of S. aureus directly from milk samples instead of classical culture. However, false-negative results may occur in the polymerase chain reaction analysis performed directly from clinical material. For the purpose of disclosing the false negative results, the use of internal amplification controls can be beneficial. Therefore, in this study a new polymerase chain reaction technique with host specific internal amplification control was developed by optimizing S. aureus-specific primers in combination with bovine specific primers. The effectiveness of the developed technique in this study was attempted in milk samples from bovine subclinical mastitis. This technique has the potential to detect S. aureus from bovine milk samples or dairy products.

PCR Assay With Host Specific Internal Control for Staphylococcus Aureus From Bovine Milk Samples

Mustafa Kemal University, Faculty of Veterinary Medicine, Department of Microbiology, Tayfur Sokmen Campus 31000 Hatay, Turkey

Mustafa Kemal University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, Tayfur Sokmen Campus 31000 Hatay, Turkey

Yüzüncü Yıl University, Faculty of Pharmacy, Department of Pharmaceutical Microbiology, Campus, 65100 Van, Turkey

Department of Agronomy, Telidji Amar University, P. O. Box 37G, 03000 Laghouat, LBRA, Algeria

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Staphylococcus aureus is considered as one of the most important and common pathogens of bovine mastitis. Polymerase Chain Reaction is frequently proposed in...

PCR Assay With Host Specific Internal Control for Staphylococcus Aureus From Bovine Milk Samples

Published Online: Nov 18, 2015

Page range: 97 - 100

Received: Nov 13, 2014

Accepted: Jan 20, 2015

DOI: https://doi.org/10.14432/j.macvetrev.2015.01.038

Keywords
bovine milk, internal control, polymerase chain reaction, Staphylococcus aureus

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

PCR Assay With Host Specific Internal Control for Staphylococcus Aureus From Bovine Milk Samples

Published Online: Nov 18, 2015

Page range: 97 - 100

Received: Nov 13, 2014

Accepted: Jan 20, 2015

DOI: https://doi.org/10.14432/j.macvetrev.2015.01.038

Keywordsbovine milk, internal control, polymerase chain reaction, Staphylococcus aureus

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
bovine milk, internal control, polymerase chain reaction, Staphylococcus aureus