<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The aim of this study is to compare the results from the histopathology and the immunohistochemical method in the diagnostic of Porcine circovirus type 2 (PCV2) infection in pigs. The circovirus infection is a pig disease that is caused by a small, spherical, nonenveloped virus with a single stranded DNA genome which is spread throughout the pig industry worldwide. The circovirus is the etiological agent of a several pig diseases which today are thought to be the cause of the greatest economical loses in pig production. The most important of these diseases is the PMWS (post-weaning multisystemic wasting syndrome). In this article we have performed an investigation of four farms on which there had been a previous clinical diagnosis of the Post-weaning multisystemic wasting syndrome. The examination was performed on thirty pigs from these farms, from two to five months old, which had the most severe symptoms of the disease. Necropsy, histopathology and immunohistochemical diagnostic methods were performed. The most significant necropsy findings were the enlarged lymph nodes (especially the inguinal, mediasinal and the mesenteric lymph nodes). The main histopathological changes were located in the lymphatic organs presented by B and T lymphocyte depletion and increase in the number of the macrophages. PCV2 antigen and nucleic acid were detected in almost all of the examined tissues. The examination showed that the histopathological and immunohistochemical methods provide complementary results in diagnosing PCV2 in pigs.</p></abstract>

The aim of this study is to compare the results from the histopathology and the immunohistochemical method in the diagnostic of Porcine circovirus type 2 (PCV2) infection in pigs. The circovirus infection is a pig disease that is caused by a small, spherical, nonenveloped virus with a single stranded DNA genome which is spread throughout the pig industry worldwide. The circovirus is the etiological agent of a several pig diseases which today are thought to be the cause of the greatest economical loses in pig production. The most important of these diseases is the PMWS (post-weaning multisystemic wasting syndrome). In this article we have performed an investigation of four farms on which there had been a previous clinical diagnosis of the Post-weaning multisystemic wasting syndrome. The examination was performed on thirty pigs from these farms, from two to five months old, which had the most severe symptoms of the disease. Necropsy, histopathology and immunohistochemical diagnostic methods were performed. The most significant necropsy findings were the enlarged lymph nodes (especially the inguinal, mediasinal and the mesenteric lymph nodes). The main histopathological changes were located in the lymphatic organs presented by B and T lymphocyte depletion and increase in the number of the macrophages. PCV2 antigen and nucleic acid were detected in almost all of the examined tissues. The examination showed that the histopathological and immunohistochemical methods provide complementary results in diagnosing PCV2 in pigs.

Diagnostic Characteristics of Circovirus Infection in Pigs

Faculty of Veterinary Medicine, “Ss Cyril and Methodius” University in Skopje Lazar Pop Trajkov 5-7, 1000 Skopje, Republic of Macedonia

Department of Pathology and Forensic Medicine Faculty of Veterinary Medicine-Skopje “Ss. Cyril and Methodius” University in Skopje Lazar Pop Trajkov 5-7, 1000 Skopje, Republic of Macedonia

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Diagnostic Characteristics of Circovirus Infection in Pigs"}

The aim of this study is to compare the results from the histopathology and the immunohistochemical method in the diagnostic of Porcine circovirus type 2...

Diagnostic Characteristics of Circovirus Infection in Pigs

Published Online: Nov 18, 2015

Page range: 73 - 78

Received: Jul 21, 2014

Accepted: Nov 21, 2014

DOI: https://doi.org/10.14432/j.macvetrev.2014.11.033

Keywords
histopathology, immunohistochemistry, porcine circovirus type 2, post-weaning multisystemic wasting syndrome

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Diagnostic Characteristics of Circovirus Infection in Pigs

Published Online: Nov 18, 2015

Page range: 73 - 78

Received: Jul 21, 2014

Accepted: Nov 21, 2014

DOI: https://doi.org/10.14432/j.macvetrev.2014.11.033

Keywordshistopathology, immunohistochemistry, porcine circovirus type 2, post-weaning multisystemic wasting syndrome

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
histopathology, immunohistochemistry, porcine circovirus type 2, post-weaning multisystemic wasting syndrome