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Journal of Electrical Bioimpedance
Volume 3 (2012): Numero 1 (January 2012)
Accesso libero
Transient impedance changes in venous endothelial monolayers as a biological radiation dosimetry response
Erik F. Young
Erik F. Young
| 23 ott 2012
Journal of Electrical Bioimpedance
Volume 3 (2012): Numero 1 (January 2012)
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Article Category:
Articles
Pubblicato online:
23 ott 2012
Pagine:
61 - 65
Ricevuto:
26 lug 2012
DOI:
https://doi.org/10.5617/jeb.395
Parole chiave
Bioimpedance
,
endothelium
,
ECIS
,
radiation
,
dosimetry
,
HUVEC
© 2012 Erik F. Young, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Fig.1
Radiation induced changes in venous endothelial monolayer permeability via ECIS. Primary human umbilical cord vein endothelial cells (HUVECs) were seeded on collagen coated 8W10E+ arrays and allowed to mature into competent monolayers prior to irradiation. Transmonolayer resistance for the entire course of culture is shown in A. The trace shows contact, spreading, sealing and decline of the monolayer’s cells over time. Changes in resistance after irradiation shown in B reveal a significant decrease (**, p = 0.017) in monolayer resistance 3 h after irradiation. The dominant contributor to this resistance is the modeled Rb parameter (C) that reported a statistically significant (*, p = 0.038) change in resistance arising from lateral cell contacts. The mean and standard deviation of three monolayers is plotted. Radiation induces an early, transient decrease in endothelial monolayer permeability.
Fig.2
Time-lapse video microscopy of irradiated primary HUVECs. Cells were seeded on collagen-coated substrate and allowed to mature into confluent monolayers before irradiation with 5.0 Gy of γ rays. Frames from the video timecourse (Supplementary Movies S2,S3) were captured after irradiation at the intervals shown. Endothelial monolayers are dynamic with dividing and motile cells and are not overtly compromised after radiation. Scale bars; 200 μ.