<abstract xmlns="http://www.w3.org/1999/xhtml">

<italic>Schizosaccharomyces pombe</italic> cells of strains each carrying a deletion of one of the genes <italic>snf5</italic>, <italic>ypa1</italic>, <italic>pho7</italic> and <italic>pas1</italic> and of a strain overexpressing gene <italic>odr1</italic>, have been previously shown to grow in presence of the toxic glucose analogue 2-deoxyglucose (2-DG). Here we report that these genes control 2-DG induced lysis and are, with the exception of <italic>odr1</italic>, also involved in control of formation of reactive oxygen species (ROS) upon exposure of cells to H2O2. Lysis of deletion strains, but not of strain overexpressing <italic>odr1</italic>, is dependent on glucose concentration of the medium whereas ROS formation is glucose independent.
</abstract>

Schizosaccharomyces pombe cells of strains each carrying a deletion of one of the genes snf5, ypa1, pho7 and pas1 and of a strain overexpressing gene odr1, have been previously shown to grow in presence of the toxic glucose analogue 2-deoxyglucose (2-DG). Here we report that these genes control 2-DG induced lysis and are, with the exception of odr1, also involved in control of formation of reactive oxygen species (ROS) upon exposure of cells to H2O2. Lysis of deletion strains, but not of strain overexpressing odr1, is dependent on glucose concentration of the medium whereas ROS formation is glucose independent.

Genes Controlling 2-deoxyglucose Induced Lysis and Formation of Reactive Oxygen Species in <italic>Schizosaccharomyces pombe</italic>

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Genes Controlling 2-deoxyglucose Induced Lysis and Formation of Reactive Oxygen Species in 

Schizosaccharomyces pombe cells of strains each carrying a deletion of one of the genes snf5, ypa1, pho7 and pas1 and of a strain overexpressing gene odr1,...

Genes Controlling 2-deoxyglucose Induced Lysis and Formation of Reactive Oxygen Species in Schizosaccharomyces pombe

Pubblicato online: 27 set 2017

Pagine: 393 - 396

Ricevuto: 07 set 2016

Accettato: 12 gen 2017

DOI: https://doi.org/10.5604/01.3001.0010.4877

Parole chiave
2-deoxyglucose, fission yeast, glucose signaling, lysis, ROS formation

© 2017 AKSHAY VISHWANATHA et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Genes Controlling 2-deoxyglucose Induced Lysis and Formation of Reactive Oxygen Species in Schizosaccharomyces pombe

Pubblicato online: 27 set 2017

Pagine: 393 - 396

Ricevuto: 07 set 2016

Accettato: 12 gen 2017

DOI: https://doi.org/10.5604/01.3001.0010.4877

Parole chiave2-deoxyglucose, fission yeast, glucose signaling, lysis, ROS formation

© 2017 AKSHAY VISHWANATHA et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Parole chiave
2-deoxyglucose, fission yeast, glucose signaling, lysis, ROS formation