Coomassie Blue G250 for Visualization of Active Bacteria from Lake Environment and Culture

Amann R., F.O. Glöckner and A. Neef. 1997. Modern methods in subsurface microbiology: In situ identification of microorganisms with nucleic acid probes. FEMS Microbiology Reviews. 20: 191–200.10.1111/j.1574-6976.1997.tb00308.xSearch in Google Scholar

Ammor M.S., A.B. Flórez, A. Margolles and B. Mayo. 2006. Fluorescence spectroscopy: a rapid tool for assessing tetracycline resistance in Bifidobacterium longum. Can. J. Microbiol. 52: 740–746.10.1139/w06-031Search in Google Scholar

Bastviken D. and L. Tranvik. 2001. The Leucine Incorporation method estimates bacterial growth equally well in both oxic and anoxic lake waters. Appl. Environ. Microbiol. 67: 2916–2921.10.1128/AEM.67.7.2916-2921.2001Search in Google Scholar

Bengtsson M.M., K. Sjøtun, A. Lanzén and L. Øvreås. 2012. Bacterial diversity in relation to secondary production and succession on surfaces of the kelp Laminaria hyperborea. ISME J. 6: 2188–2198.10.1038/ismej.2012.67 Search in Google Scholar

Böllmann J., K. Rathsack and M. Martienssen. 2016. The precision of bacterial quantification techniques on different kinds of environmental samples and the effect of ultrasonic treatment. J. Microbiol. Methods 126: 42–47.10.1016/j.mimet.2016.05.006 Search in Google Scholar

Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.10.1016/0003-2697(76)90527-3 Search in Google Scholar

Carlsson N., C.C. Kitts and B. Åkerman. 2012. Spectroscopic characterization of Coomassie blue and its binding to amyloid fibrils. Anal. Biochem. 420: 33–40.10.1016/j.ab.2011.08.04321945461 Search in Google Scholar

Chróst R.J. 1990. Microbial ectoenzymes in aquatic environments, pp. 47–78. In: Overbeck J. and J.R. Chróst (eds.). Aquatic Microbial Ecology: Biochemical and Molecular Approaches. Springer, New York.10.1007/978-1-4612-3382-4_3 Search in Google Scholar

Chróst R.J. and H. Rai. 1993. Bacterial secondary production, pp. 92–117. In: Overbeck J. and R.J. Chróst (eds.). Microbial Ecology of Lake Pluβsee. Springer-Verlag, New York.10.1007/978-1-4612-2606-2_5 Search in Google Scholar

Franklin T.J. and G.A. Snow. 2005. Facilitated uptake of antimicrobial drugs, pp. 129–135. In: Franklin T.J. and G.A. Snow (eds.). Biochemistry and molecular biology of antimicrobial drug action, biochemistry and molecular biology of antimicrobial. Springer Science- l-Business, Media, Inc., NY10.1007/0-387-27566-5 Search in Google Scholar

Freese H.M., U. Karsten and R. Schumann. 2006. Bacterial abundance, activity, and viability in the eutrophic River Warnow, northeast Germany. Microb. Ecol. 51: 117–127. Search in Google Scholar

Fuhrman J.A. and F. Azam. 1982. Thymidine incorporation as a measure of heterotrophic bacterioplankton production in marine surface waters: Evaluation and field results. Mar. Biol. 66: 109–120.10.1007/BF00397184 Search in Google Scholar

Georgiou C.D., K. Grintzalis, G. Zervoudakis and I. Papaposto- lou. 2008. Mechanism of Coomassie brilliant blue G-250 binding to proteins: A hydrophobic assay for nanogram quantities of proteins. Anal. Bioanal. Chem. 391: 391–403.10.1007/s00216-008-1996-x Search in Google Scholar

Glazier S.A. and J.J. Horvath. 1995. Feasibility of fluorescence detection of tetracycline in media mixtures employing a fiber optic probe. Anal. Lett. 28: 2607–2624.10.1080/00032719508007413 Search in Google Scholar

Gottesman S. and M.R. Maurizi. 1992. Regulation by proteolysis: energy-dependent proteases and their targets. Microbiol. Rev. 56: 592–621.10.1128/mr.56.4.592-621.1992 Search in Google Scholar

Griebler C., B. Mindle and D. Slezak. 2001. Combining DAPI and SYBR Green II for the enumeration of total bacteria numbers in aquatic sediments. Internat. Rev. Hydrobiol. 86: 453–465.10.1002/1522-2632(200107)86:4/5<453::AID-IROH453>3.0.CO;2-L Search in Google Scholar

Haglund A.L., P. Lantz, E. Törnblom and L. Tranvik. 2003. Depth distribution of active bacteria and bacterial activity in lake sediment. FEMS Microbiol. Ecol. 46: 31–38.10.1016/S0168-6496(03)00190-9 Search in Google Scholar

Karner M. and J.A. Fuhrman. 1997. Determination of active marine bacterioplankton: a comparison of universal 16S rRNA probes, autoradiography, and nucleoid staining. Appl. Environ. Microbiol. 63: 1208–1213.10.1128/aem.63.4.1208-1213.1997 Search in Google Scholar

Katrahalli U., S.S. Kalanur and J. Seetharamappa. 2010. Interaction of bioactive Coomassie Brilliant Blue G with protein: insights from spectroscopic methods. Sci. Pharm. 78: 869–880.10.3797/scipharm.1008-15 Search in Google Scholar

Knoll S., W. Zwisler and M. Simon. 2001. Bacterial colonization of early stages of limnetic diatom microaggregates. Aquat. Microb. Ecol. 25: 141–150.10.3354/ame025141 Search in Google Scholar

Kiersztyn B., W. Siuda and R.J. Chróst. 2012. Persistence of bacterial proteolytic enzymes in lake ecosystems. FEMS Microbiol. Ecol. 80: 124–134.10.1111/j.1574-6941.2011.01276.x Search in Google Scholar

Larimer C., E. Winder, R. Jeters, M. Prowant, I. Nettleship, R.S. Addleman and G.T. Bonheyo. 2016. A method for rapid quantitative assessment of biofilms with biomolecular staining and image analysis. Anal. Bioanal. Chem. 408: 999–1008.10.1007/s00216-015-9195-z Search in Google Scholar

Lebaron P., N. Parthuisot and P. Catala. 1998. Comparison of blue nuclei acid dyes for flow cytometric enumeration of bacteria in aquatic systems. Appl. Environ. Microbiol. 64: 1725–1730.10.1128/AEM.64.5.1725-1730.1998 Search in Google Scholar

Long R.A. and F. Azam. 1996. Abundant protein-containing particles in the sea. Aquat. Microb. Ecol. 10: 213–221.10.3354/ame010213 Search in Google Scholar

Luna G.M., E. Mannini and R. Donovaro. 2002. Large fraction of dead and inactive bacteria in coastal marine sediments: comparison of protocols for determination and ecological significance. Appl. Environ. Microbiol. 68: 3509–3513.10.1128/AEM.68.7.3509-3513.2002 Search in Google Scholar

Luo S., N.B. Wehr and R.L. Levine. 2006. Quantitation of protein on gels and blots by infrared fluorescence of Coomassie Blue and Fast Green. Anal. Biochem. 350: 233–238.10.1016/j.ab.2005.10.048 Search in Google Scholar

Neumann U., H. Khalaf and M. Rimpler. 1994. Quantitation of electrophoretically separated proteins in the submicrogram range by dye elution. Electrophoresis 15: 916–921.10.1002/elps.11501501132 Search in Google Scholar

Penzkofer A. and Y. Lu. 1986. Fluorescence quenching of rhodamine 6G in methanol at high concentration. Chem. Phys. 103: 399–405.10.1016/0301-0104(86)80041-6 Search in Google Scholar

Porter K.G. and Y.S. Feig. 1980. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 25: 943–948.10.4319/lo.1980.25.5.0943 Search in Google Scholar

Rodriguez G.G., D. Phipps, K. Ishiguro and H.F. Ridgway. 1992. Use of a fluorescent redox probe for direct visualization of actively respiring bacteria. Appl. Environ. Microbiol. 58: 1801–1808.10.1128/aem.58.6.1801-1808.19921956871622256 Search in Google Scholar

Shibata A., K. Kogure, I. Koike and K. Ohwada. 1997. Formation of submicron colloidal particles from marine bacteria by viral infection. Mar. Ecol. Prog. Ser. 155: 303–307.10.3354/meps155303 Search in Google Scholar

Simon M. 1988. Growth Characteristics of small and large free- living and attached bacteria in Lake Constance. Microb. Ecol. 15: 151–163.10.1007/BF02011709 Search in Google Scholar

Simon M. and F. Azam. 1989. Protein content and protein synthesis rates of planktonic marine bacteria. Mar. Ecol. Prog. Ser. 51: 201–213.10.3354/meps051201 Search in Google Scholar

Smith E.M. and P.A. del Giorgio. 2003. Low fractions of active bacteria in natural aquatic communities? Aquat. Microb. Ecol. 31: 203–208.10.3354/ame031203 Search in Google Scholar

Suller M.T.E. and D. Lloyd. 1999. Fluorescence monitoring of antibiotic-induced bacterial damage using flow cytometry. Cytometry 35: 235–241.10.1002/(SICI)1097-0320(19990301)35:3<235::AID-CYTO6>3.0.CO;2-0 Search in Google Scholar

Tupas L.M., B.N. Popp and D.M. Karl. 1994. Dissolved organic carbon in oligotrophic waters: experiments on sample preservation, storage and analysis. Mar. Chem. 45: 207–216. Search in Google Scholar

Warkentin M., H.M. Freese, U. Karsten and R. Schumann. 2007. New and fast method to quantify respiration rates of bacterial and plankton communities in freshwater ecosystems by using optical oxygen sensor spots. Appl. Environ. Microbiol. 73: 6722–6729.10.1128/AEM.00405-07 Search in Google Scholar

Zweifel U.L. and A. Hagstrom. 1995. Total counts of marine bacteria include a large fraction of non-nucleoid- containing bacteria (ghosts). Appl. Environ. Microbiol. 61: 2180–2185.10.1128/aem.61.6.2180-2185.1995Search in Google Scholar