<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: Umbilical cord-derived mesenchymal stem cells (UC-MSCs) are considered to be multipotent mesenchymal stem cells that are easily induced to differentiate into functional osteoblasts both in vitro and in vivo. Osterix (Osx), a novel zinc-⃞nger-containing transcription factor of the Sp family, is required for osteoblast differentiation and bone formation.Objective: We investigated the effect of Osx on the proliferation and osteogenic differentiation of the UC-MSCs.Method: The primary UC-MSCs were isolated and cultured. An Osx-expressing plasmid (pEGFP-Osx) was constructed and transfected into UC-MSCs. Then expression of bone morphogenesis-related genes, proliferation rate, alkaline phosphatase activity, and mineralization were examined to evaluate the osteogenic potential of the Osx gene-modified UC-MSCs.Result: UC-MSCs transfected with pEGFP-Osx exhibited apparent osteogenic differentiation as determined by increased activity of alkaline phosphatase, the formation of mineralized nodules and the expression of related osteoblastic genes.Conclusion: These results confirmed the ability of Osx to enhance osteoblast differentiation of UC-MSCs in vitro, and the Osx gene-modified UC-MSCs are potential as novel cell resources of bone tissue engineering.</abstract>

Background: Umbilical cord-derived mesenchymal stem cells (UC-MSCs) are considered to be multipotent mesenchymal stem cells that are easily induced to differentiate into functional osteoblasts both in vitro and in vivo. Osterix (Osx), a novel zinc-⃞nger-containing transcription factor of the Sp family, is required for osteoblast differentiation and bone formation.Objective: We investigated the effect of Osx on the proliferation and osteogenic differentiation of the UC-MSCs.Method: The primary UC-MSCs were isolated and cultured. An Osx-expressing plasmid (pEGFP-Osx) was constructed and transfected into UC-MSCs. Then expression of bone morphogenesis-related genes, proliferation rate, alkaline phosphatase activity, and mineralization were examined to evaluate the osteogenic potential of the Osx gene-modified UC-MSCs.Result: UC-MSCs transfected with pEGFP-Osx exhibited apparent osteogenic differentiation as determined by increased activity of alkaline phosphatase, the formation of mineralized nodules and the expression of related osteoblastic genes.Conclusion: These results confirmed the ability of Osx to enhance osteoblast differentiation of UC-MSCs in vitro, and the Osx gene-modified UC-MSCs are potential as novel cell resources of bone tissue engineering.

Osteogenic differentiation of human umbilical cord-derived mesenchymal stem cells promoted by overexpression of osterix

Department of Oral and Maxillofacial Surgery, Provincial Hospital Affiliated to Shandong University, Shandong, Jinan, 250021,

Department of Orthodontics, Provincial Hospital Affiliated to Shandong University, Shandong, Jinan 250021,

Department of Oral Radiology, Provincial Hospital Affiliated to Shandong University, Shandong, Jinan 250021,

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Background: Umbilical cord-derived mesenchymal stem cells (UC-MSCs) are considered to be multipotent mesenchymal stem cells that are easily induced to...

Osteogenic differentiation of human umbilical cord-derived mesenchymal stem cells promoted by overexpression of osterix

Article Category: Original article

Pubblicato online: 04 feb 2017

Pagine: 743 - 752

DOI: https://doi.org/10.5372/1905-7415.0706.236

Parole chiave
Osteogenic differentiation, osterix, umbilical cord-derived mesenchymal stem cells

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Osteogenic differentiation of human umbilical cord-derived mesenchymal stem cells promoted by overexpression of osterix

Article Category: Original article

Pubblicato online: 04 feb 2017

Pagine: 743 - 752

DOI: https://doi.org/10.5372/1905-7415.0706.236

Parole chiaveOsteogenic differentiation, osterix, umbilical cord-derived mesenchymal stem cells

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Parole chiave
Osteogenic differentiation, osterix, umbilical cord-derived mesenchymal stem cells