<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: Some Thai vegetables may enhance the activities of some phase II enzymes and decrease activities of phase I enzymes. Thus, they may possess cancer chemo-preventive potentials.Objective: Determine the anti-clastogenic activity of Thai vegetable, Siamese cassia (SC), against an indirectacting clastogens, cyclophosphamide (CYP), and 7, 12-dimethylbenz(a)anthracene (DMBA).Methods: Male mice were fed either with semi-purified diet, containing 2% or 4% of ground lyophilized SC leaves, for two weeks prior to administration of clastogens. The anti-clastogenicity of SC leaves using the in vivo erythrocyte micronucleus assay in mice was performed. Blood samples were collected and counted for reticulocytes with and without a micronucleus using the fluorescent microscope.Results: Feeding SC leaves at 2% or 4% in the diets reduced the number of micronucleated peripheral reticulocytes (MNRETs) induced by both CYP and DMBA. However, the effect was statistically significant only at 4% in CYP-induced mice.Conclusion: Siamese cassia leaves possess anti-clastogenic activity against clastogens in mice, particularly in a high dose.</abstract>

Background: Some Thai vegetables may enhance the activities of some phase II enzymes and decrease activities of phase I enzymes. Thus, they may possess cancer chemo-preventive potentials.Objective: Determine the anti-clastogenic activity of Thai vegetable, Siamese cassia (SC), against an indirectacting clastogens, cyclophosphamide (CYP), and 7, 12-dimethylbenz(a)anthracene (DMBA).Methods: Male mice were fed either with semi-purified diet, containing 2% or 4% of ground lyophilized SC leaves, for two weeks prior to administration of clastogens. The anti-clastogenicity of SC leaves using the in vivo erythrocyte micronucleus assay in mice was performed. Blood samples were collected and counted for reticulocytes with and without a micronucleus using the fluorescent microscope.Results: Feeding SC leaves at 2% or 4% in the diets reduced the number of micronucleated peripheral reticulocytes (MNRETs) induced by both CYP and DMBA. However, the effect was statistically significant only at 4% in CYP-induced mice.Conclusion: Siamese cassia leaves possess anti-clastogenic activity against clastogens in mice, particularly in a high dose.

Anticlastogenic potential of Thai vegetable, Siamese cassia, using mouse erythrocyte micronucleus assay

Laboratory Animal Section,Research Division, National Cancer Institute, Bangkok 10400,

Biochemistry and Chemical Carcinogenesis Section, Research Division, National Cancer Institute, Bangkok 10400,

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Background: Some Thai vegetables may enhance the activities of some phase II enzymes and decrease activities of phase I enzymes. Thus, they may possess cancer...

Anticlastogenic potential of Thai vegetable, Siamese cassia, using mouse erythrocyte micronucleus assay

Article Category: Brief communication (Original)

Pubblicato online: 04 feb 2017

Pagine: 669 - 673

DOI: https://doi.org/10.5372/1905-7415.0505.087

Parole chiave
Anticlastogenic, micronucleus, MNRETs, Siamese cassia

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Anticlastogenic potential of Thai vegetable, Siamese cassia, using mouse erythrocyte micronucleus assay

Article Category: Brief communication (Original)

Pubblicato online: 04 feb 2017

Pagine: 669 - 673

DOI: https://doi.org/10.5372/1905-7415.0505.087

Parole chiaveAnticlastogenic, micronucleus, MNRETs, Siamese cassia

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Parole chiave
Anticlastogenic, micronucleus, MNRETs, Siamese cassia