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Negative Pressure Wound Therapy (NPWT) has been widely adopted in wound healing strategies due to its multimodal mechanism of action. While NPWT’s positive impression on wound healing is well-established, its effect on bacterial load reduction remains equivocal. This study investigates NPWT’s efficacy in reducing bioburden using an <italic>in vitro</italic> porcine skin model, focusing on the impact of <italic>Staphylococcus aureus</italic> and <italic>Staphylococcus epidermidis</italic>. Custom-made negative pressure chambers were employed to apply varying negative pressures. Porcine skin was cut into 5 × 5 cm squares and three standardized wounds of 6 mm each were created using a biopsy punch. Then, wounds were infected with <italic>S. aureus</italic> and <italic>S. epidermidis</italic> bacterial suspensions diluted 1:10,000 to obtain a final concentration of 1.5 × 104 CFU/ml and were placed in negative pressure chambers. After incubation, bacterial counts were expressed as colony-forming units (CFU) per ml. For <italic>S. aureus</italic> at 120 hours, the median CFU, mean area per colony, and total growth area were notably lower at −80 mmHg when compared to −250 mmHg and −50 mmHg, suggesting an optimal negative pressure for the pressure-dependent inhibition of the bacterial proliferation. While analyzing <italic>S. epidermidis</italic> at 120 hours, the response to the negative pressure was similar but less clear, with the minor CFU at −100 mmHg. The influence of intermittent negative pressure on the <italic>S. epidermidis</italic> growth showed notably lower median CFU with the interval therapy every hour compared to the <italic>S. aureus</italic> control group. This study contributes valuable insights into NPWT’s influence on the bacterial load, emphasizing the need for further research to reformulate its role in managing contaminated wounds.
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</abstract>

Negative Pressure Wound Therapy (NPWT) has been widely adopted in wound healing strategies due to its multimodal mechanism of action. While NPWT’s positive impression on wound healing is well-established, its effect on bacterial load reduction remains equivocal. This study investigates NPWT’s efficacy in reducing bioburden using an in vitro porcine skin model, focusing on the impact of Staphylococcus aureus and Staphylococcus epidermidis. Custom-made negative pressure chambers were employed to apply varying negative pressures. Porcine skin was cut into 5 × 5 cm squares and three standardized wounds of 6 mm each were created using a biopsy punch. Then, wounds were infected with S. aureus and S. epidermidis bacterial suspensions diluted 1:10,000 to obtain a final concentration of 1.5 × 104 CFU/ml and were placed in negative pressure chambers. After incubation, bacterial counts were expressed as colony-forming units (CFU) per ml. For S. aureus at 120 hours, the median CFU, mean area per colony, and total growth area were notably lower at −80 mmHg when compared to −250 mmHg and −50 mmHg, suggesting an optimal negative pressure for the pressure-dependent inhibition of the bacterial proliferation. While analyzing S. epidermidis at 120 hours, the response to the negative pressure was similar but less clear, with the minor CFU at −100 mmHg. The influence of intermittent negative pressure on the S. epidermidis growth showed notably lower median CFU with the interval therapy every hour compared to the S. aureus control group. This study contributes valuable insights into NPWT’s influence on the bacterial load, emphasizing the need for further research to reformulate its role in managing contaminated wounds.

Negative Pressure Level and Effects on Bacterial Growth Kinetics in an <italic>in vitro</italic> Wound Model

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Negative Pressure Level and Effects on Bacterial Growth Kinetics in an  Wound Model

Negative Pressure Wound Therapy (NPWT) has been widely adopted in wound healing strategies due to its multimodal mechanism of action. While NPWT’s positive...

Negative Pressure Level and Effects on Bacterial Growth Kinetics in an in vitro Wound Model

Article Category: Original Paper

Pubblicato online: 20 giu 2024

Pagine: 199 - 206

Ricevuto: 18 feb 2024

Accettato: 20 apr 2024

DOI: https://doi.org/10.33073/pjm-2024-018

Parole chiave
negative pressure wound therapy, bacterial growth, wound healing, bioburden

© 2024 Adam Bobkiewicz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Negative Pressure Level and Effects on Bacterial Growth Kinetics in an in vitro Wound Model

Article Category: Original Paper

Pubblicato online: 20 giu 2024

Pagine: 199 - 206

Ricevuto: 18 feb 2024

Accettato: 20 apr 2024

DOI: https://doi.org/10.33073/pjm-2024-018

Parole chiavenegative pressure wound therapy, bacterial growth, wound healing, bioburden

© 2024 Adam Bobkiewicz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Parole chiave
negative pressure wound therapy, bacterial growth, wound healing, bioburden