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Kestose Increases the Relative Abundance of Faecalibacterium spp. and Nominally Increases Cow Milk Tolerant Dose in Children with Cow's Milk Allergy – Preliminary Results

Shohei Kubota
Shohei Kubota
, 
Shiro Sugiura
Shiro Sugiura
, 
Mayuko Takahashi
Mayuko Takahashi
, 
Yoshihiro Kadota
Yoshihiro Kadota
, 
Yoshihiro Takasato
Yoshihiro Takasato
, 
Teruaki Matsui
Teruaki Matsui
, 
Katsumasa Kitamura
Katsumasa Kitamura
, 
Takumi Tochio
Takumi Tochio
 e 
Komei Ito
Komei Ito
   | 20 set 2023
Polish Journal of Microbiology's Cover Image
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Article Category: Original Paper
Pubblicato online: 20 set 2023
Pagine: 299 - 306
Ricevuto: 24 mar 2023
Accettato: 30 giu 2023
DOI: https://doi.org/10.33073/pjm-2023-030
Parole chiave
© 2023 Shohei Kubota et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Changes in the patients’ immunological parameters.The laboratory data from 10 participants before and after the study are shown. a) Total Immunoglobulin E (tIgE), b) milk-specific Immunoglobulin E (sIgE), c) casein-specific Immunoglobulin G4 (IgG4), d) Thymus and Activation-Regulated Chemokine (TARC). No significant changes were seen after 12 weeks (p > 0.05, Wilcoxon's signed-rank test).
Changes in the patients’ immunological parameters.The laboratory data from 10 participants before and after the study are shown. a) Total Immunoglobulin E (tIgE), b) milk-specific Immunoglobulin E (sIgE), c) casein-specific Immunoglobulin G4 (IgG4), d) Thymus and Activation-Regulated Chemokine (TARC). No significant changes were seen after 12 weeks (p > 0.05, Wilcoxon's signed-rank test).

Fig. 2.

Relative abundance of gut microbiota at 0 and 12 weeks.This bar graph shows the composition of the intestinal microbiota in each of the 10 subjects. The numbers on the horizontal axis of the graphs represent the patient number, and the graphs in the same column represent the results for the same subject. The results for the top 17 genera with the highest occupancy are shown. The top row shows the results at the beginning of the study, and the bottom row shows the results 12 weeks into the study.
Relative abundance of gut microbiota at 0 and 12 weeks.This bar graph shows the composition of the intestinal microbiota in each of the 10 subjects. The numbers on the horizontal axis of the graphs represent the patient number, and the graphs in the same column represent the results for the same subject. The results for the top 17 genera with the highest occupancy are shown. The top row shows the results at the beginning of the study, and the bottom row shows the results 12 weeks into the study.

Fig. 3.

Absolute quantification of intestinal microbiota (qPCR).The logarithm of the genome copy number of Faecalibacterium prausnitzii per gram of feces is shown at baseline (0w) and after 12 weeks of intervention (12w). Dotted lines connect changes in the same participants. Eight of 10 patients exhibited nominal increases from 0 to 12 weeks, but this difference was not statistically significant (p > 0.05, Wilcoxon's signed-rank test).
Absolute quantification of intestinal microbiota (qPCR).The logarithm of the genome copy number of Faecalibacterium prausnitzii per gram of feces is shown at baseline (0w) and after 12 weeks of intervention (12w). Dotted lines connect changes in the same participants. Eight of 10 patients exhibited nominal increases from 0 to 12 weeks, but this difference was not statistically significant (p > 0.05, Wilcoxon's signed-rank test).

Relative abundance of gut microbiota.

0w 12w p-value*
Median (25%–75%) Median (25%–75%)
xBifidobacterium 16.2 (8.3–24.2) 21.7 (13.3–24.1) 0.72
Blautia 9.5 (6.8–16.9) 13.6 (10.0–22.7) 0.24
Bacteroides 4.0 (0.8–8.3) 6.6 (3.7–9.8) 0.20
Faecalibacterium 3.8 (1.4–7.0) 6.8 (4.4–9.7) 0.01
Fusicatenibacter 6.0 (0.3–8.1) 5.3 (0.4–8.8) 0.29
Anaerostipes 5.0 (1.0–7.7) 4.4 (2.3–7.3) 0.39
Roseburia 3.8 (0.5–5.3) 0.8 (0.4–8.0) 0.88
Lachnospiracea_incertae_sedis 3.0 (2.7–5.3) 3.8 (2.5–4.5) 0.65
Gemmiger 4.6 (1.0–7.5) 4.7 (1.1–5.6) 0.67
Streptococcus 2.3 (0.9–6.5) 2.4 (1.6–4.7) 0.88
Clostridium XlVa 2.9 (1.1–4.5) 2.2 (1.8–3.9) 0.24
Ruminococcus2 0.6 (0.4–3.1) 0.8 (0.3–1.7) 0.51
Veillonella 1.8 (0.2–3.4) 0.8 (0.2–3.2) 0.96
Clostridium XI 1.4 (0.1–3.0) 0.6 (0.4–0.9) 0.33
Dialister 0.2 (0.0–2.2) 0.9 (0.0–1.9) 0.59
Butyricicoccus 0.4 (0.1–1.3) 0.9 (0.6–1.6) 0.20
Clostridium IV 0.6 (0.1–1.5) 0.3 (0.2–1.0) 0.45

Background information on the participants.

Number of participants 10
Age (median/minimum-maximum) 8/2–12
Male/female 7/3
Atopic dermatitis 8
Bronchial asthma 7
Allergic rhinitis 8
IgE (total) IU/ml (25%–75%) 1,157 (384–2,744)
IgE (milk) kUA/l (25%–75%) 50 (27–66)
EASI score (25%–75%) 0.3 (0.05–2.1)
Serum TARC (pg/ml) (25%–75%) 551 (333–976)

Oral food challenge results for all patients.

First OFC Second OFC
TD (ml) TS (points) TS/Pro TD (ml) TS (points) TS/Pro (points/g)
Patient 1 3.5 10 94 8.5 5 19
Patient 2 1.5 25 550 3.5 25 236
Patient 3 1.7 15 291 3.7 1 9
Patient 4 1.7 31 601 0.7 20 943
Patient 5 0.1 10 3300 1.8 0 0
Patient 6 0.8 16 660 1.8 20 367
Patient 7 3.7 15 134 3.7 15 134
Patient 8 0.1 10 3300 0.3 25 2750
Patient 9 0.7 11 519 1.7 20 388
Patient 10 3.7 15 134 3.7 40 357
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