Accesso libero

Physicochemical and Microbiological Water Quality Assessment of a Northwestern Algerian Dam: Detection of Ichtyopathogenic Bacteria

Hanane Boumerdassi
Hanane Boumerdassi
, 
Lydia Neïla Djouadi
Lydia Neïla Djouadi
, 
Abderrahmane Hambli
Abderrahmane Hambli
, 
Marie-Laure Fardeau
Marie-Laure Fardeau
, 
Hadda-Imen Ouzari
Hadda-Imen Ouzari
 e 
Farida Nateche
Farida Nateche
   | 14 giu 2023
Polish Journal of Microbiology's Cover Image
INFORMAZIONI SU QUESTO ARTICOLO
Articolo precedente
Articolo Successivo
Cita
Article Category: ORIGINAL PAPER
Pubblicato online: 14 giu 2023
Pagine: 187 - 198
Ricevuto: 27 gen 2023
Accettato: 14 apr 2023
DOI: https://doi.org/10.33073/pjm-2023-020
Parole chiave
© 2023 Hanane Boumerdassi et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Geographical location of Sekkak dam.
Geographical location of Sekkak dam.

Fig. 2.

Seasonal variations of the physicochemical parameters of the Sekkak dam. T – temperature (°C), pH – potential hydrogen, DO2 – dissolved oxygen (%), OM – organic matter (mg/l), BOD5 – biological oxygen demand 5 (mg/l), COD – chemical oxygen demand (mg/l), NO3– – nitrates (mg/l), NO2– – nitrites (mg/l), NH4+ – ammonium (mg/l), Ds – dry residue (mg/l), PO43– – phosphorus (mg/l), SD – standard deviation
Seasonal variations of the physicochemical parameters of the Sekkak dam. T – temperature (°C), pH – potential hydrogen, DO2 – dissolved oxygen (%), OM – organic matter (mg/l), BOD5 – biological oxygen demand 5 (mg/l), COD – chemical oxygen demand (mg/l), NO3– – nitrates (mg/l), NO2– – nitrites (mg/l), NH4+ – ammonium (mg/l), Ds – dry residue (mg/l), PO43– – phosphorus (mg/l), SD – standard deviation

Fig. 3.

Seasonal variations of different bacterial groups.
Seasonal variations of different bacterial groups.

Fig. 4.

Neighbor-joining phylogenetic tree of Gram-negative bacteria strains isolated from Sekkak dam using 16S rRNA sequences.
Neighbor-joining phylogenetic tree of Gram-negative bacteria strains isolated from Sekkak dam using 16S rRNA sequences.

Fig. 5.

Neighbor-joining phylogenetic tree of Gram-positive bacteria strains isolated from Sekkak dam using 16S rRNA sequences.
Neighbor-joining phylogenetic tree of Gram-positive bacteria strains isolated from Sekkak dam using 16S rRNA sequences.

Fig. 6.

Antibiotic resistance frequencies and percentage of resistant strains for each antibiotic. penicillins (beta-lactams family) cephalosporins (beta-lactams family) polymyxins family. aminoglycosides family. macrolides family. tetracyclins family. streptogramins family. novobiocin
Antibiotic resistance frequencies and percentage of resistant strains for each antibiotic. penicillins (beta-lactams family) cephalosporins (beta-lactams family) polymyxins family. aminoglycosides family. macrolides family. tetracyclins family. streptogramins family. novobiocin

Characterization and identification of isolates.

Strains Catalase Oxydase Gram Identification
S33, S41• + − − Acinetobacter johnsonii* Gram- (n = 45 stains)
S60, S58 + + − Aeromonas hydrophila*
S43• + + − Alcaligenes aquatilis
S32• + + − Comamonas testosteroni
S40, S39• + − − Citrobacter freundii*
S28, S62 + − − Edwardsiella tarda*
S1, S10, S5, S2, S9• + − − Enterobacter cloacae
S42 + − − Klebsiella sp.
S44 + + − Photobacterium damselae
S23, S34, S35 + − − Proteus mirabilis*
S26 + − − Proteus vulgaris*
S30, S29, S27 + − − Providencia rettgeri*
S21• + − − Providencia vermicola*
S11, S18, S16 + + − Pseudomonas aeruginosa*
S24, S31 + + − Pseudomonas fluorescens*
S17, S12 + + − Pseudomonas lactis
S15, S19 + + − Pseudomonas luteola*
S6, S36, S61• + − − Serratia fonticola
S4, S56, S38 + + − Vibrio alginolyticus*
S57, S37 + + − Vibrio fluvialis*
S14, S13, S59• + + − Psychrobacter pulmonis*
S55 − − + Aerococcus urinaeequi) Gram+ (n = 17 stains
S8• + + + Bacillus flexus
S54 − − + Enterococcus faecalis*
S51, S3•, S50• + − + Leucobacter aridicollis
S46• + + + Salinicoccus roseus
S7• + + + Sporosarcina aquimarina
S20 + + + Sporosarcina globispora
S22• + + + Sporosarcina newyorkensis
S25• − − + Vagococcus fluvialis
S53, S52• + − + Microbacterium maritypicum
S48, S49• + − + Staphylococcus equorum subsp. linens
S45•, S47 + − + Staphylococcus aureus*
Total 62 strains

Pattern of antibiotic resistance of isolates.

Strains Number of antibiotic Patterns
Klebsiella sp. 3 OX AMP / E
Edwardsiella tarda 4 CX OX AMP / K
Proteus vulgaris 4 OX AMP CJ / E
Proteus mirabilis 4 CX OX AMP CJ
Edwardsiella tarda 5 CX OX AMP / K / NV
Proteus mirabilis 5 CX OX AMP CJ / NV
Proteus mirabilis 5 CX OX AMP AX CJ
Enterobacter cloacae 5 CX OX TI CJ CEP
Serratia fonticola 5 CX TI CEP / E / NV
Pseudomonas lactis 6 CX OX AMP CJ CFP / E
Pseudomonas lactis 6 CX OX AMP CJ / E / VI
Vibrio fluvialis 6 CX OX AMP AX TI NV
Pseudomonas aeruginosa 6 CX OX TI CJ CEP CTR
Pseudomonas luteola 6 CX OX AMP CJ CFP CAZ
Acinetobacter johnsonii 6 CX OX AMP AX TI CJ
Enterobacter cloacae 6 CX OX TI CJ CEP / VI
Vibrio fluvialis 6 CX OX AMP AX CFP / NV
Psychrobacter pulmonis 7 CX OX AMP AX TI CFP CAZ
Enterococcus faecalis 7 CX OX AMP AX CFP / CS / O
Salinicoccus roseus 7 CX AMP AX CFP CAZ / CS / O
Pseudomonas luteola 7 CX OX AMP CJ CFP CAZ / E
Vibrio alginolyticus 7 CX OX AMP AX CEP / K / NV
Vibrio alginolyticus 7 CX OX AMP AX CFP CEP / K
Pseudomonas aeruginosa 7 CX OX TI CJ CEP CFP CTR
Enterobacter cloacae 7 CX OX TI CJ CEP / E / O
Providencia rettgeri 7 CX OX AMP AX TI CJ / CS
Serratia fonticola 7 CX TI CEP CFP / GM / E / VI
Pseudomonas fluorescens 7 CX OX TI CJ CEP CTR / E
Enterobacter cloacae 7 CX OX TI CJ CEP / O / VI
Bacillus flexus 8 CX TI CEP CFP CAZ / CS / GM / VI
Staphylococcus equorum subsp. linens 8 CX AMP AX TI CEP CTX / CS / NV
Microbacterium maritypicum 8 CX OX AMP AX TI CJ CEP / CS
Acinetobacter johnsonii Providencia rettgeri 8 CX OX AMP AX TI CJ / CS / NV
Sporosarcina aquimarina Sporosarcina globispora 8 CX CFP CAZ CTR / CS / GM / E / VI
Citrobacter freundii 8 CX OX AMP AX TI CJ CEP / O
Pseudomonas aeruginosa 8 CX OX TI CJ CEP CFP CAZ CTR
Vibrio alginolyticus 8 CX OX AMP AX CFP CEP / K / NV
Aerococcus urinaeequi 9 CX OX TI CEP CFP CAZ / CS / GM / VI
Aeromonas hydrophila 9 CX OX AMP CJ CAZ / CS / E / O / VI
Aeromonas hydrophila 9 CX OX AMP AX CJ / CS / E / O / VI
Staphylococcus aureus 9 CX OX AMP AX CEP CTX / CS / O / NV
Staphylococcus aureus 9 CX OX AMP AX TI CEP CTX / CS / O
Citrobacter freundii 9 CX OX AMP AX CJ CEP CTX / CS / O
Comamonas testosteroni 9 CX OX AMP AX TI CEP CAZ / TOB / O
Serratia fonticola 9 CX TI CEP CFP CAZ / GM / E / VI / NV
Vagococcus fluvialis 10 CX OX TI CJ CEP CTX CFP / CS / GM / VI
Pseudomonas fluorescens 10 CX OX TI CJ CEP CFP CTR / CS / TOB / E
Leucobacter aridicollis 10 CX OX TI CJ CEP CFP CAZ CTR / CS / O
Staphylococcus equorum subsp. linens 10 CX AMP AX TI CEP CTX CFP CAZ / CS / NV
Psychrobacter pulmonis 10 CX OX AMP AX TI CJ CEP CFP CAZ / O
Providencia vermicola 10 CX OX AMP AX CJ CFP CAZ / E / O / NV
Enterobacter cloacae 10 CX OX CJ CEP CFP CAZ / GM / E / VI / NV
Psychrobacter pulmonis 11 CX TI CJ CEP CFP CAZ CTR / GM / E / VI / NV
Photobacterium damselae 11 CX TI CJ CEP CFP CAZ / GM / K / E / O / NV
Microbacterium maritypicum 11 CX OX AMP AX TI CJ CEP CFP CAZ / CS / O
Leucobacter aridicollis 11 CX OX TI CJ CEP CFP CAZ CTR / CS / GM / O
Leucobacter aridicollis 11 CX OX AMP AX TI CEP CFP CTR CAZ / CS / O
Alcaligenes aquatilis 12 CX OX AMP AX TI CEP CFP CAZ / E / O / VI / NV
Sporosarcina newyorkensis 13 CX OX TI CJ CEP CFP CAZ CTR / CS / E / O / VI / NV
Providencia rettgeri 14 CX OX AMP AX TI CJ CFP CAZ / CS / TOB / E / O / VI / NV
eISSN:
2544-4646
Lingua:
Inglese
Frequenza di pubblicazione:
4 volte all'anno
Argomenti della rivista:
Life Sciences, Microbiology and Virology
Feed RSS della rivista