Treatment | Phage P2 adsorption (%) (mean ± S.D.) |
---|---|
none (control) | 97.26 ± 1.21a |
1% SDS (30 min, 37°C) | 98.26 ± 4.08a |
50 U/ml lysozyme (30 min, 37°C) | 75.78 ± 3.01b |
0.1 mg/ml proteinase K (30 min, 37°C) | 97.80 ± 1.81a |
5% TCA (15 min, 100°C) | 57.14 ± 6.25c |
CDS | Strand | Predicted function | Function |
---|---|---|---|
CDS12 | + | terminase small subunit | packaging |
CDS14 | + | terminase large subunit | |
CDS15 | + | portal protein | |
CDS16 | + | prohead protease | structure |
CDS17 | + | major capsid protein | |
CDS18 | + | putative tail protein | |
CDS20 | + | head-tail joining protein | |
CDS21 | + | head-tail adaptor | |
CDS22 | + | tail protein | |
CDS23 | + | major tail protein | |
CDS25 | + | tape measure protein | |
CDS26 | + | distal tail protein | |
CDS27 | + | baseplate protein tail-like protein | |
CDS28 | + | tail fiber protein | |
CDS57 | – | membrane protein | |
CDS35 | + | integrase | host interaction |
CDS59 | – | ATP/GTP- binding protein | regulation |
CDS48 | – | PemK family transcriptional regulator | |
CDS83 | + | putative DNA binding protein | |
CDS37 | – | DNA polymerase | DNA replication |
CDS58 | – | DNA polymerase | |
CDS72 | + | DNA helicase | |
CDS73 | + | DNA primase | |
CDS74 | + | single-stranded-DNA-specific exonuclease | |
CDS1 | – | HNH endonuclease | |
CDS3 | – | HNH endonuclease | |
CDS11 | + | HNH endonuclease | |
CDS38 | – | HNH endonuclease | |
CDS41 | – | HNH endonuclease | |
CDS45 | – | HNH endonuclease | |
CDS56 | – | HNH endonuclease | |
CDS65 | – | HNH homing endonuclease | |
tRNA | + | tRNA-Pro | |
tRNA | + | tRNA-Gly | |
CDS44 | – | extracellular transglycosylase | additional function |
CDS69 | + | deoxynucleoside kinase | |
CDS96 | thymidine kinase |