In vitro Effects of Prebiotics and Synbiotics on Apis cerana Gut Microbiota

Abdel-Moneim AME, Elbaz AM, Khidr RES, Badri FB. Effect of in ovo inoculation of Bifidobacterium spp. on growth performance, thyroid activity, ileum histomorphometry, and microbial enumeration of broilers. Probiotics Antimicrob Proteins. 2020 Sep; 12(3):873–882. https://doi.org/10.1007/s12602-019-09613-xAbdel-MoneimAMEElbazAMKhidrRESBadriFB. Effect of in ovo inoculation of Bifidobacterium spp. on growth performance, thyroid activity, ileum histomorphometry, and microbial enumeration of broilers. Probiotics Antimicrob Proteins. 2020Sep; 12(3):873–882. https://doi.org/10.1007/s12602-019-09613-x31709505Search in Google Scholar

Audisio MC, Sabaté DC, Benítez-Ahrendts MR. Effect of Lactobacillus johnsonii CRL1647 on different parameters of honeybee colonies and bacterial populations of the bee gut. Benef Microbes. 2015 Oct 15;6(5):687–695. https://doi.org/10.3920/BM2014.0155AudisioMCSabatéDCBenítez-AhrendtsMR. Effect of Lactobacillus johnsonii CRL1647 on different parameters of honeybee colonies and bacterial populations of the bee gut. Benef Microbes. 2015Oct15;6(5):687–695. https://doi.org/10.3920/BM2014.015525809216Search in Google Scholar

Carlson J, Erickson J, Hess J, Gould T, Slavin J. Prebiotic dietary fiber and gut health: comparing the in vitro fermentations of beta-glucan, inulin and xylooligosaccharide. Nutrients. 2017 Dec 15; 9(12):1361. https://doi.org/10.3390/nu9121361CarlsonJEricksonJHessJGouldTSlavinJ. Prebiotic dietary fiber and gut health: comparing the in vitro fermentations of beta-glucan, inulin and xylooligosaccharide. Nutrients. 2017Dec15; 9(12):1361. https://doi.org/10.3390/nu9121361574881129244718Search in Google Scholar

Chen C, Liu Z, Luo Y, Xu Z, Wang S, Zhang X, Dai R, Gao J, Chen X, Guo H, et al. Managed honeybee colony losses of the Eastern honeybee (Apis cerana) in China (2011–2014). Apidologie. 2017 Sep;48(5):692–702. https://doi.org/10.1007/s13592-017-0514-6ChenCLiuZLuoYXuZWangSZhangXDaiRGaoJChenXGuoH. Managed honeybee colony losses of the Eastern honeybee (Apis cerana) in China (2011–2014). Apidologie. 2017Sep;48(5):692–702. https://doi.org/10.1007/s13592-017-0514-6Search in Google Scholar

Dai P, Yan Z, Ma S, Yang Y, Wang Q, Hou C, Wu Y, Liu Y, Diao Q. The herbicide glyphosate negatively affects midgut bacterial communities and survival of honey bee during larvae reared in vitro. J Agric Food Chem. 2018 Jul 25;66(29):7786–7793. https://doi.org/10.1021/acs.jafc.8b02212DaiPYanZMaSYangYWangQHouCWuYLiuYDiaoQ. The herbicide glyphosate negatively affects midgut bacterial communities and survival of honey bee during larvae reared in vitro. J Agric Food Chem. 2018Jul25;66(29):7786–7793. https://doi.org/10.1021/acs.jafc.8b0221229992812Search in Google Scholar

Driessen FM, Deboer R. Fermented milks with selected intestinal bacteria – a healthy trend in new products. Neth Milk Dairy J. 1989;43(3):367–382.DriessenFMDeboerR. Fermented milks with selected intestinal bacteria – a healthy trend in new products. Neth Milk Dairy J. 1989;43(3):367–382.Search in Google Scholar

Duan X, Zhao B, Jin X, Cheng X, Huang S, Li J. Antibiotic treatment decrease the fitness of honeybee (Apis mellifera) larvae. Insects. 2021 Mar 30;12(4):301. https://doi.org/10.3390/insects12040301DuanXZhaoBJinXChengXHuangSLiJ. Antibiotic treatment decrease the fitness of honeybee (Apis mellifera) larvae. Insects. 2021Mar30;12(4):301. https://doi.org/10.3390/insects12040301806630533808048Search in Google Scholar

Ellegaard KM, Tamarit D, Javelind E, Olofsson TC, Andersson SGE, Vásquez A. Extensive intra-phylotype diversity in lactobacilli and bifidobacteria from the honeybee gut. BMC Genomics. 2015 Dec;16(1):284. https://doi.org/10.1186/s12864-015-1476-6EllegaardKMTamaritDJavelindEOlofssonTCAnderssonSGEVásquezA. Extensive intra-phylotype diversity in lactobacilli and bifidobacteria from the honeybee gut. BMC Genomics. 2015Dec;16(1):284. https://doi.org/10.1186/s12864-015-1476-6444960625880915Search in Google Scholar

Fehlbaum S, Prudence K, Kieboom J, Heerikhuisen M, van den Broek T, Schuren F, Steinert R, Raederstorff D. In vitro fermentation of selected prebiotics and their effects on the composition and activity of the adult gut microbiota. Int J Mol Sci. 2018 Oct 10;19(10):3097. https://doi.org/10.3390/ijms19103097FehlbaumSPrudenceKKieboomJHeerikhuisenMvan den BroekTSchurenFSteinertRRaederstorffD. In vitro fermentation of selected prebiotics and their effects on the composition and activity of the adult gut microbiota. Int J Mol Sci. 2018Oct10;19(10):3097. https://doi.org/10.3390/ijms19103097621361930308944Search in Google Scholar

Fünfhaus A, Ebeling J, Genersch E. Bacterial pathogens of bees. Curr Opin Insect Sci. 2018 Apr;26:89–96. https://doi.org/10.1016/j.cois.2018.02.008FünfhausAEbelingJGenerschE. Bacterial pathogens of bees. Curr Opin Insect Sci. 2018Apr;26:89–96. https://doi.org/10.1016/j.cois.2018.02.00829764667Search in Google Scholar

Ge Y, Jing Z, Diao Q, He JZ, Liu YJ. Host species and geography differentiate honeybee gut bacterial communities by changing the relative contribution of community assembly processes. MBio. 2021 Jun 29;12(3):e0075121. https://doi.org/10.1128/mBio.00751-21GeYJingZDiaoQHeJZLiuYJ. Host species and geography differentiate honeybee gut bacterial communities by changing the relative contribution of community assembly processes. MBio. 2021Jun29;12(3):e0075121. https://doi.org/10.1128/mBio.00751-21826299634061602Search in Google Scholar

Gibson GR, Hutkins R, Sanders ME, Prescott SL, Reimer RA, Salminen SJ, Scott K, Stanton C, Swanson KS, Cani PD, et al. Expert consensus document: the International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nat Rev Gastroenterol Hepatol. 2017 Aug;14(8):491–502. https://doi.org/10.1038/nrgastro.2017.75GibsonGRHutkinsRSandersMEPrescottSLReimerRASalminenSJScottKStantonCSwansonKSCaniPD. Expert consensus document: the International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nat Rev Gastroenterol Hepatol. 2017Aug;14(8):491–502. https://doi.org/10.1038/nrgastro.2017.75Search in Google Scholar

Gilliam M, Prest DB. Fungi isolated from the intestinal contents of foraging worker honey bees, Apis mellifera. J Invertebr Pathol. 1972 Jul;20(1):101–103. https://doi.org/10.1016/0022-2011(72)90087-0GilliamMPrestDB. Fungi isolated from the intestinal contents of foraging worker honey bees, Apis mellifera. J Invertebr Pathol. 1972Jul;20(1):101–103. https://doi.org/10.1016/0022-2011(72)90087-0Search in Google Scholar

Gmeiner M, Kneifel W, Kulbe KD, Wouters R, De Boever P, Nollet L, Verstraete W. Influence of a synbiotic mixture consisting of Lactobacillus acidophilus 74-2 and a fructooligosaccharide preparation on the microbial ecology sustained in a simulation of the human intestinal microbial ecosystem (SHIME reactor). Appl Microbiol Biotechnol. 2000 Feb 1;53(2):219–223. https://doi.org/10.1007/s002530050011GmeinerMKneifelWKulbeKDWoutersRDe BoeverPNolletLVerstraeteW. Influence of a synbiotic mixture consisting of Lactobacillus acidophilus 74-2 and a fructooligosaccharide preparation on the microbial ecology sustained in a simulation of the human intestinal microbial ecosystem (SHIME reactor). Appl Microbiol Biotechnol. 2000Feb1;53(2):219–223. https://doi.org/10.1007/s00253005001110709985Search in Google Scholar

Goffin D, Delzenne N, Blecker C, Hanon E, Deroanne C, Paquot M. Will isomalto-oligosaccharides, a well-established functional food in Asia, break through the European and American market? The status of knowledge on these prebiotics. Crit Rev Food Sci Nutr. 2011 Apr 14;51(5):394–409. https://doi.org/10.1080/10408391003628955GoffinDDelzenneNBleckerCHanonEDeroanneCPaquotM. Will isomalto-oligosaccharides, a well-established functional food in Asia, break through the European and American market? The status of knowledge on these prebiotics. Crit Rev Food Sci Nutr. 2011Apr14;51(5):394–409. https://doi.org/10.1080/1040839100362895521491266Search in Google Scholar

Henrique-Bana FC, Wang X, Costa GN, Spinosa WA, Miglioranza LHS, Scorletti E, Calder PC, Byrne CD, Gibson GR. In vitro effects of Bifidobacterium lactis-based synbiotics on human faecal bacteria. Food Res Int. 2020 Feb;128(Feb):108776. https://doi.org/10.1016/j.foodres.2019.108776Henrique-BanaFCWangXCostaGNSpinosaWAMiglioranzaLHSScorlettiECalderPCByrneCDGibsonGR. In vitro effects of Bifidobacterium lactis-based synbiotics on human faecal bacteria. Food Res Int. 2020Feb;128(Feb):108776. https://doi.org/10.1016/j.foodres.2019.10877631955747Search in Google Scholar

Houdelet C, Sinpoo C, Chantaphanwattana T, Voisin SN, Bocquet M, Chantawannakul P, Bulet P. Proteomics of anatomical sections of the gut of Nosema-infected Western Honeybee (Apis mellifera) reveals different early responses to Nosema spp. isolates. J Proteome Res. 2021 Jan 01;20(1):804–817. https://doi.org/10.1021/acs.jproteome.0c00658HoudeletCSinpooCChantaphanwattanaTVoisinSNBocquetMChantawannakulPBuletP. Proteomics of anatomical sections of the gut of Nosema-infected Western Honeybee (Apis mellifera) reveals different early responses to Nosema spp. isolates. J Proteome Res. 2021Jan01;20(1):804–817. https://doi.org/10.1021/acs.jproteome.0c0065833305956Search in Google Scholar

Khalifa SAM, Elshafiey EH, Shetaia AA, El-Wahed AAA, Algethami AF, Musharraf SG, AlAjmi MF, Zhao C, Masry SHD, Abdel-Daim MM, et al. Overview of bee pollination and its economic value for crop production. Insects. 2021 Jul 31;12(8):688. https://doi.org/10.3390/insects12080688KhalifaSAMElshafieyEHShetaiaAAEl-WahedAAAAlgethamiAFMusharrafSGAlAjmiMFZhaoCMasrySHDAbdel-DaimMM. Overview of bee pollination and its economic value for crop production. Insects. 2021Jul31;12(8):688. https://doi.org/10.3390/insects12080688839651834442255Search in Google Scholar

Klein AM, Vaissière BE, Cane JH, Steffan-Dewenter I, Cunningham SA, Kremen C, Tscharntke T. Importance of pollinators in changing landscapes for world crops. Proc Biol Sci. 2007 Feb 07; 274(1608):303–313. https://doi.org/10.1098/rspb.2006.3721KleinAMVaissièreBECaneJHSteffan-DewenterICunninghamSAKremenCTscharntkeT. Importance of pollinators in changing landscapes for world crops. Proc Biol Sci. 2007Feb07; 274(1608):303–313. https://doi.org/10.1098/rspb.2006.3721170237717164193Search in Google Scholar

Koleva P, Ketabi A, Valcheva R, Gänzle MG, Dieleman LA. Chemically defined diet alters the protective properties of fructo-oligosaccharides and isomalto-oligosaccharides in HLA-B27 transgenic rats. PLoS One. 2014 Nov 4;9(11):e111717. https://doi.org/10.1371/journal.pone.0111717KolevaPKetabiAValchevaRGänzleMGDielemanLA. Chemically defined diet alters the protective properties of fructo-oligosaccharides and isomalto-oligosaccharides in HLA-B27 transgenic rats. PLoS One. 2014Nov4;9(11):e111717. https://doi.org/10.1371/journal.pone.0111717421976725369019Search in Google Scholar

Kwong WK, Moran NA. Gut microbial communities of social bees. Nat Rev Microbiol. 2016 Jun;14(6):374–384. https://doi.org/10.1038/nrmicro.2016.43KwongWKMoranNA. Gut microbial communities of social bees. Nat Rev Microbiol. 2016Jun;14(6):374–384. https://doi.org/10.1038/nrmicro.2016.43564834527140688Search in Google Scholar

Lei Q, Wang S, Yin H, Cheng Y, Yu H, Pan H, Lin Q, Cao Z. [Effects of Lactobacillus reuteri LP4 on the survival rate, intestinal microbiota composition and gut antimicrobial peptide gene expression in adult workers of Apis cerana Fabricius] (in Chinese). J Yunnan Agric Univ (Nat Sci). 2020;35(05):796–803.LeiQWangSYinHChengYYuHPanHLinQCaoZ. [Effects of Lactobacillus reuteri LP4 on the survival rate, intestinal microbiota composition and gut antimicrobial peptide gene expression in adult workers of Apis cerana Fabricius] (in Chinese). J Yunnan Agric Univ (Nat Sci). 2020;35(05):796–803.Search in Google Scholar

Likotrafiti E, Tuohy KM, Gibson GR, Rastall RA. An in vitro study of the effect of probiotics, prebiotics and synbiotics on the elderly faecal microbiota. Anaerobe. 2014 Jun;27:50–55. https://doi.org/10.1016/j.anaerobe.2014.03.009LikotrafitiETuohyKMGibsonGRRastallRA. An in vitro study of the effect of probiotics, prebiotics and synbiotics on the elderly faecal microbiota. Anaerobe. 2014Jun;27:50–55. https://doi.org/10.1016/j.anaerobe.2014.03.00924685554Search in Google Scholar

Logtenberg MJ, Akkerman R, Hobé RG, Donners KMH, Van Leeuwen SS, Hermes GDA, Haan BJ, Faas MM, Buwalda PL, Zoetendal EG, et al. Structure-specific fermentation of galacto-oligosaccharides, isomalto-oligosaccharides and isomalto/malto-polysaccharides by infant fecal microbiota and impact on dendritic cell cytokine responses. Mol Nutr Food Res. 2021 Aug;65(16):2001077. https://doi.org/10.1002/mnfr.202001077LogtenbergMJAkkermanRHobéRGDonnersKMHVan LeeuwenSSHermesGDAHaanBJFaasMMBuwaldaPLZoetendalEG. Structure-specific fermentation of galacto-oligosaccharides, isomalto-oligosaccharides and isomalto/malto-polysaccharides by infant fecal microbiota and impact on dendritic cell cytokine responses. Mol Nutr Food Res. 2021Aug;65(16):2001077. https://doi.org/10.1002/mnfr.202001077845927334060703Search in Google Scholar

Molly K, De Smet I, Nollet L, Vande Woestyne M, Verstraete W. Effect of lactobacilli on the ecology of the gastro-intestinal microbiota cultured in the SHIME reactor. Microb Ecol Health Dis. 1996 Mar;9(2):79–89. https://doi.org/10.3109/08910609609166446MollyKDe SmetINolletLVande WoestyneMVerstraeteW. Effect of lactobacilli on the ecology of the gastro-intestinal microbiota cultured in the SHIME reactor. Microb Ecol Health Dis. 1996Mar;9(2):79–89. https://doi.org/10.3109/08910609609166446Search in Google Scholar

Nainu F, Masyita A, Bahar MA, Raihan M, Prova SR, Mitra S, Emran TB, Simal-Gandara J. Pharmaceutical prospects of bee products: special focus on anticancer, antibacterial, antiviral, and antiparasitic properties. Antibiotics (Basel). 2021 Jul 06;10(7):822. https://doi.org/10.3390/antibiotics10070822NainuFMasyitaABaharMARaihanMProvaSRMitraSEmranTBSimal-GandaraJ. Pharmaceutical prospects of bee products: special focus on anticancer, antibacterial, antiviral, and antiparasitic properties. Antibiotics (Basel). 2021Jul06;10(7):822. https://doi.org/10.3390/antibiotics10070822830084234356743Search in Google Scholar

Okazaki Y, Katayama T. Consumption of non-digestible oligosaccharides elevates colonic alkaline phosphatase activity by up-regulating the expression of IAP-I, with increased mucins and microbial fermentation in rats fed a high-fat diet. Br J Nutr. 2019 Jan 28;121(2):146–154. https://doi.org/10.1017/S0007114518003082OkazakiYKatayamaT. Consumption of non-digestible oligosaccharides elevates colonic alkaline phosphatase activity by up-regulating the expression of IAP-I, with increased mucins and microbial fermentation in rats fed a high-fat diet. Br J Nutr. 2019Jan28;121(2):146–154. https://doi.org/10.1017/S000711451800308230400998Search in Google Scholar

Ortiz-Alvarado Y, Clark DR, Vega-Melendez CJ, Flores-Cruz Z, Domingez-Bello MG, Giray T. Antibiotics in hives and their effects on honey bee physiology and behavioral development. Biol Open. 2020 Jan 01;9(11):bio.053884. https://doi.org/10.1242/bio.053884Ortiz-AlvaradoYClarkDRVega-MelendezCJFlores-CruzZDomingez-BelloMGGirayT. Antibiotics in hives and their effects on honey bee physiology and behavioral development. Biol Open. 2020Jan01;9(11):bio.053884. https://doi.org/10.1242/bio.053884771000933106276Search in Google Scholar

Pachla A, Ptaszyńska AA, Wicha M, Kunat M, Wydrych J, Oleńska E, Małek W. Insight into probiotic properties of lactic acid bacterial endosymbionts of Apis mellifera L. derived from the Polish apiary. Saudi J Biol Sci. 2021 Mar;28(3):1890–1899. https://doi.org/10.1016/j.sjbs.2020.12.040PachlaAPtaszyńskaAAWichaMKunatMWydrychJOleńskaEMałekW. Insight into probiotic properties of lactic acid bacterial endosymbionts of Apis mellifera L. derived from the Polish apiary. Saudi J Biol Sci. 2021Mar;28(3):1890–1899. https://doi.org/10.1016/j.sjbs.2020.12.040793819233732075Search in Google Scholar

Pandey KR, Naik SR, Vakil BV. Probiotics, prebiotics and synbiotics – a review. J Food Sci Technol. 2015 Dec;52(12):7577–7587. https://doi.org/10.1007/s13197-015-1921-1PandeyKRNaikSRVakilBV. Probiotics, prebiotics and synbiotics – a review. J Food Sci Technol. 2015Dec;52(12):7577–7587. https://doi.org/10.1007/s13197-015-1921-1464892126604335Search in Google Scholar

Poeker SA, Geirnaert A, Berchtold L, Greppi A, Krych L, Steinert RE, de Wouters T, Lacroix C. Understanding the prebiotic potential of different dietary fibers using an in vitro continuous adult fermentation model (PolyFermS). Sci Rep. 2018 Dec;8(1):4318. https://doi.org/10.1038/s41598-018-22438-yPoekerSAGeirnaertABerchtoldLGreppiAKrychLSteinertREde WoutersTLacroixC. Understanding the prebiotic potential of different dietary fibers using an in vitro continuous adult fermentation model (PolyFermS). Sci Rep. 2018Dec;8(1):4318. https://doi.org/10.1038/s41598-018-22438-y584760129531228Search in Google Scholar

Ptaszyńska AA, Paleolog J, Borsuk G. Nosema ceranae infection promotes proliferation of yeasts in honey bee intestines. PLoS One. 2016 Oct 13;11(10):e0164477. https://doi.org/10.1371/journal.pone.0164477PtaszyńskaAAPaleologJBorsukG. Nosema ceranae infection promotes proliferation of yeasts in honey bee intestines. PLoS One. 2016Oct13;11(10):e0164477. https://doi.org/10.1371/journal.pone.0164477506336727736915Search in Google Scholar

Ramos OY, Basualdo M, Libonatti C, Vega MF. Current status and application of lactic acid bacteria in animal production systems with a focus on bacteria from honey bee colonies. J Appl Microbiol. 2020 May;128(5):1248–1260. https://doi.org/10.1111/jam.14469RamosOYBasualdoMLibonattiCVegaMF. Current status and application of lactic acid bacteria in animal production systems with a focus on bacteria from honey bee colonies. J Appl Microbiol. 2020May;128(5):1248–1260. https://doi.org/10.1111/jam.1446931566847Search in Google Scholar

Rastall RA, Gibson GR. Recent developments in prebiotics to selectively impact beneficial microbes and promote intestinal health. Curr Opin Biotechnol. 2015 Apr;32:42–46. https://doi.org/10.1016/j.copbio.2014.11.002RastallRAGibsonGR. Recent developments in prebiotics to selectively impact beneficial microbes and promote intestinal health. Curr Opin Biotechnol. 2015Apr;32:42–46. https://doi.org/10.1016/j.copbio.2014.11.00225448231Search in Google Scholar

Raymann K, Bobay LM, Moran NA. Antibiotics reduce genetic diversity of core species in the honeybee gut microbiome. Mol Ecol. 2018 Apr;27(8):2057–2066. https://doi.org/10.1111/mec.14434RaymannKBobayLMMoranNA. Antibiotics reduce genetic diversity of core species in the honeybee gut microbiome. Mol Ecol. 2018Apr;27(8):2057–2066. https://doi.org/10.1111/mec.14434593554929164717Search in Google Scholar

Raymann K, Shaffer Z, Moran NA. Antibiotic exposure perturbs the gut microbiota and elevates mortality in honeybees. PLoS Biol. 2017 Mar 14;15(3):e2001861. https://doi.org/10.1371/journal.pbio.2001861RaymannKShafferZMoranNA. Antibiotic exposure perturbs the gut microbiota and elevates mortality in honeybees. PLoS Biol. 2017Mar14;15(3):e2001861. https://doi.org/10.1371/journal.pbio.2001861534942028291793Search in Google Scholar

Rycroft CE, Jones MR, Gibson GR, Rastall RA. A comparative in vitro evaluation of the fermentation properties of prebiotic oligosaccharides. J Appl Microbiol. 2001 Nov 23;91(5):878–887. https://doi.org/10.1046/j.1365-2672.2001.01446.xRycroftCEJonesMRGibsonGRRastallRA. A comparative in vitro evaluation of the fermentation properties of prebiotic oligosaccharides. J Appl Microbiol. 2001Nov23;91(5):878–887. https://doi.org/10.1046/j.1365-2672.2001.01446.x11722666Search in Google Scholar

Sanders ME, Merenstein DJ, Reid G, Gibson GR, Rastall RA. Probiotics and prebiotics in intestinal health and disease: from biology to the clinic. Nat Rev Gastroenterol Hepatol. 2019 Oct;16(10):605–616. https://doi.org/10.1038/s41575-019-0173-3SandersMEMerensteinDJReidGGibsonGRRastallRA. Probiotics and prebiotics in intestinal health and disease: from biology to the clinic. Nat Rev Gastroenterol Hepatol. 2019Oct;16(10):605–616. https://doi.org/10.1038/s41575-019-0173-331296969Search in Google Scholar

Śliżewska K, Chlebicz A. Synbiotics impact on dominant faecal microbiota and short-chain fatty acids production in sows. FEMS Microbiol Lett. 2019 Jul;366(13): fnz157. https://doi.org/10.1093/femsle/fnz157ŚliżewskaKChlebiczA. Synbiotics impact on dominant faecal microbiota and short-chain fatty acids production in sows. FEMS Microbiol Lett. 2019Jul;366(13): fnz157. https://doi.org/10.1093/femsle/fnz15731295345Search in Google Scholar

Sorndech W, Nakorn KN, Tongta S, Blennow A. Isomalto-oligosaccharides: recent insights in production technology and their use for food and medical applications. LWT Food Sci Technol. 2018 Sep; 95:135–142. https://doi.org/10.1016/j.lwt.2018.04.098SorndechWNakornKNTongtaSBlennowA. Isomalto-oligosaccharides: recent insights in production technology and their use for food and medical applications. LWT Food Sci Technol. 2018Sep; 95:135–142. https://doi.org/10.1016/j.lwt.2018.04.098Search in Google Scholar

Swanson KS, Gibson GR, Hutkins R, Reimer RA, Reid G, Verbeke K, Scott KP, Holscher HD, Azad MB, Delzenne NM, et al. The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of synbiotics. Nat Rev Gastroenterol Hepatol. 2020 Nov;17(11):687–701. https://doi.org/10.1038/s41575-020-0344-2SwansonKSGibsonGRHutkinsRReimerRAReidGVerbekeKScottKPHolscherHDAzadMBDelzenneNM. The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of synbiotics. Nat Rev Gastroenterol Hepatol. 2020Nov;17(11):687–701. https://doi.org/10.1038/s41575-020-0344-2758151132826966Search in Google Scholar

Tandon D, Haque MM, Gote M, Jain M, Bhaduri A, Dubey AK, Mande SS. A prospective randomized, double-blind, placebo-controlled, dose-response relationship study to investigate efficacy of fructo-oligosaccharides (FOS) on human gut microflora. Sci Rep. 2019 Dec;9(1):5473. https://doi.org/10.1038/s41598-019-41837-3TandonDHaqueMMGoteMJainMBhaduriADubeyAKMandeSS. A prospective randomized, double-blind, placebo-controlled, dose-response relationship study to investigate efficacy of fructo-oligosaccharides (FOS) on human gut microflora. Sci Rep. 2019Dec;9(1):5473. https://doi.org/10.1038/s41598-019-41837-3644508830940833Search in Google Scholar

Tornero-Martínez A, Cruz-Ortiz R, Jaramillo-Flores ME, Osorio-Díaz P, Ávila-Reyes SV, Alvarado-Jasso GM, Mora-Escobedo R. In vitro fermentation of polysaccharides from Aloe vera and the evaluation of antioxidant activity and production of short chain fatty acids. Molecules. 2019 Oct 07;24(19):3605. https://doi.org/10.3390/molecules24193605Tornero-MartínezACruz-OrtizRJaramillo-FloresMEOsorio-DíazPÁvila-ReyesSVAlvarado-JassoGMMora-EscobedoR. In vitro fermentation of polysaccharides from Aloe vera and the evaluation of antioxidant activity and production of short chain fatty acids. Molecules. 2019Oct07;24(19):3605. https://doi.org/10.3390/molecules24193605680390131591306Search in Google Scholar

Wang L, Hu L, Yan S, Jiang T, Fang S, Wang G, Zhao J, Zhang H, Chen W. Effects of different oligosaccharides at various dosages on the composition of gut microbiota and short-chain fatty acids in mice with constipation. Food Funct. 2017;8(5):1966–1978. https://doi.org/10.1039/C7FO00031FWangLHuLYanSJiangTFangSWangGZhaoJZhangHChenW. Effects of different oligosaccharides at various dosages on the composition of gut microbiota and short-chain fatty acids in mice with constipation. Food Funct. 2017;8(5):1966–1978. https://doi.org/10.1039/C7FO00031FSearch in Google Scholar

Wang S, Gao J, Liu J, Zhao D. Morphometric characterization of Apis cerana hainana (Hymenoptera: Apidae) in Hainan province, P.R. China. J Apic Res. 2021 Mar 15;60(2):337–340. https://doi.org/10.1080/00218839.2020.1753326WangSGaoJLiuJZhaoD. Morphometric characterization of Apis cerana hainana (Hymenoptera: Apidae) in Hainan province, P.R. China. J Apic Res. 2021Mar15;60(2):337–340. https://doi.org/10.1080/00218839.2020.1753326Search in Google Scholar

Wang S. [Diversity of intestinal microbial and prebiotic function of lactic acid bacteria in Apis Cerana Fabricius] [Master Thesis] (in Chinese). Kunming (People’s Republic of China): Yunnan Agricultural University; 2018.WangS. [Diversity of intestinal microbial and prebiotic function of lactic acid bacteria in Apis Cerana Fabricius] [Master Thesis] (in Chinese). Kunming (People’s Republic of China): Yunnan Agricultural University; 2018.Search in Google Scholar

Watson D, O’Connell Motherway M, Schoterman MHC, van Neerven RJJ, Nauta A, van Sinderen D. Selective carbohydrate utilization by lactobacilli and bifidobacteria. J Appl Microbiol. 2013 Apr;114(4):1132–1146. https://doi.org/10.1111/jam.12105WatsonDO’Connell MotherwayMSchotermanMHCvan NeervenRJJNautaAvan SinderenD. Selective carbohydrate utilization by lactobacilli and bifidobacteria. J Appl Microbiol. 2013Apr;114(4):1132–1146. https://doi.org/10.1111/jam.1210523240984Search in Google Scholar

Williams NT. Probiotics. Am J Health Syst Pharm. 2010 Mar 15; 67(6):449–458. https://doi.org/10.2146/ajhp090168WilliamsNT. Probiotics. Am J Health Syst Pharm. 2010Mar15; 67(6):449–458. https://doi.org/10.2146/ajhp09016820208051Search in Google Scholar