<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The aim of this study was to assess the periodontal status of cystic fibrosis (CF) adult patients and to evaluate whether there is a correlation between the bacterial population of the subgingival biofilm and the health status of the periodontal tissues in this group of adults. The study involved 22 cystic fibrosis adult patients. The periodontal condition was assessed using Plaque Index (PLI), Gingival Index (GI), and Probing Pocket Depth (PPD). The gingival sulcus samples were analyzed by the Real-Time PCR assay (RT-PCR). Majority of patients showed moderate or severe bacterial dental plaque accumulation, but none of them had clinical symptoms of periodontal diseases. RT-PCR showed the presence of periopathogens in 50% of patients. Red complex microorganisms were detected in 9.09%, orange complex in 27.27%, and green complex in 31.82% of the samples analyzed. In cystic fibrosis patients colonized by periopathogens, the periodontal markers were significantly higher in comparison to not colonized by periopathogens patients. Despite the widespread presence of bacterial dental deposits in the cystic fibrosis adult patients examined, none of them has clinical symptoms of periodontal disease; however, the presence of periodontal pathogens in subgingival biofilm may represent a possible risk factor of this disease in the future. An unsatisfactory level of oral hygiene in any patient with cystic fibrosis indicates a need to focus on standards of dental care for such patients.</p></abstract>

The aim of this study was to assess the periodontal status of cystic fibrosis (CF) adult patients and to evaluate whether there is a correlation between the bacterial population of the subgingival biofilm and the health status of the periodontal tissues in this group of adults. The study involved 22 cystic fibrosis adult patients. The periodontal condition was assessed using Plaque Index (PLI), Gingival Index (GI), and Probing Pocket Depth (PPD). The gingival sulcus samples were analyzed by the Real-Time PCR assay (RT-PCR). Majority of patients showed moderate or severe bacterial dental plaque accumulation, but none of them had clinical symptoms of periodontal diseases. RT-PCR showed the presence of periopathogens in 50% of patients. Red complex microorganisms were detected in 9.09%, orange complex in 27.27%, and green complex in 31.82% of the samples analyzed. In cystic fibrosis patients colonized by periopathogens, the periodontal markers were significantly higher in comparison to not colonized by periopathogens patients. Despite the widespread presence of bacterial dental deposits in the cystic fibrosis adult patients examined, none of them has clinical symptoms of periodontal disease; however, the presence of periodontal pathogens in subgingival biofilm may represent a possible risk factor of this disease in the future. An unsatisfactory level of oral hygiene in any patient with cystic fibrosis indicates a need to focus on standards of dental care for such patients.

Periodontal Status and Subgingival Biofilms in Cystic Fibrosis Adults

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

{"article-title":"Periodontal Status and Subgingival Biofilms in Cystic Fibrosis Adults"}

The aim of this study was to assess the periodontal status of cystic fibrosis (CF) adult patients and to evaluate whether there is a correlation between the...

	PLI		GI		PPD		TOTAL BACTERIA COUNT (CFU/ml)
C	1.76 ± 0.70	p < 0.01	0.56 ± 0.36	p < 0.05	1.33 ± 0.24	p < 0.01	8.9 × 10⁶ ± 1.5 × 10⁷	p > 0.05
NC	0.86 ± 0.28	p < 0.01	0.21 ± 0.26	p < 0.05	0.98 ± 0.12	p < 0.01	1.8 × 10⁷ ± 5.3 × 10⁷	p > 0.05
TOTAL	1.31 ± 0.69		0.39 ± 0.36		1.16 ± 0.26		1.3 × 10⁷ ± 3.8 × 10⁷

The periopathogens tested		Number of bacteria (CFU/ml) Mean ± SD	Percentage of the periopathogens tested within the total bacterial count (%)
Aggregatibacter actinomycetemcomitans		0.00	0.00
Red complex	Porphyromonas gingivalis	2.0 × 10 ± 9.4 × 10	0.01
	Tannerella forsythia	2.4 × 10 ± 1.1 × 10²	0.01
	Treponema denticola	1.4 × 10² ± 6.3 × 10²	0.01
Orange complex	Fusobacterium nucleatum	7.3 × 10² ± 2.5 × 10³	0.93
	Prevotella intermedia	8.2 × 10³ ± 3.9 × 10⁴	0.01
	Peptostreptococcus micros	1.7 × 10³ ± 8.2 × 10³	1.50
	Eubacterium nodatum	0.00	0.00
Green complex	Capnocytophaga gingivalis	6.8 × 10⁴ ± 3.1 × 10⁵	3.51

Periodontal Status and Subgingival Biofilms in Cystic Fibrosis Adults

Article Category: original-paper

Pubblicato online: 03 set 2019

Pagine: 377 - 382

Ricevuto: 12 giu 2019

Accettato: 30 lug 2019

DOI: https://doi.org/10.33073/pjm-2019-040

Parole chiave
cystic fibrosis, periodontal pathogens, periodontitis, Real-Time PCR

© 2019 Anton Pyskun et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Clinical data of adult patients with cystic fibrosis that were colonised or not colonised by periopathogens.

The number of bacteria isolated from gingival sulcus and the percentage of the species tested within the total bacterial count in samples.

Periodontal Status and Subgingival Biofilms in Cystic Fibrosis Adults

Article Category: original-paper

Pubblicato online: 03 set 2019

Pagine: 377 - 382

Ricevuto: 12 giu 2019

Accettato: 30 lug 2019

DOI: https://doi.org/10.33073/pjm-2019-040

Parole chiavecystic fibrosis, periodontal pathogens, periodontitis, Real-Time PCR

© 2019 Anton Pyskun et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Clinical data of adult patients with cystic fibrosis that were colonised or not colonised by periopathogens.

The number of bacteria isolated from gingival sulcus and the percentage of the species tested within the total bacterial count in samples.

Parole chiave
cystic fibrosis, periodontal pathogens, periodontitis, Real-Time PCR