Paper shows (1) the results based on vegetation monitoring during the time period 1975-2005 in the grassland segment of undulated landscape along railway corridor close to village Dlouhá Třebová (Ústí nad Orlicí district, Eastern Bohemia, Czech Republic). Survey was focused on the meadow communities, identification of phycenological changes by comparison of historical phytosociological records (1975) with the present ones (2005). (2) Actual relevés taken within transect design showed that there is direct dependence between the meadow species composition and soil moisture gradient. Along the soil moisture gradient different formations can be found: