<abstract xmlns="http://www.w3.org/1999/xhtml">

<p> High performance liquid chromatography method with diode array detection (HPLC-DAD) was developed and optimised for the determination of tetracyclines (TCs) in medicated feedingstuffs. The extraction of the analyte from feedingstuffs was performed with Na<sub>2</sub>EDTA-McIlvaine buffer (pH 2.5 and pH 4). The extracts were cleaned up by solid phase extraction using octadecyl cartridges (C18). The samples were dried up and redissolved in the mixture of oxalic acid and methanol. Separation was performed on reserved phase column (Phenomenex C18, 250 x 4.6 mm, 5 μm) by multistep gradient elution, which provided better chromatographic separation. The analysis was performed at a wavelength of 390 nm. Validation study of the method revealed that all obtained calibration curves showed good linearity R= 0.9985 for doxycycline (DC) and R= 0.9981 for chlorotetracycline (CTC) over the range of 25-2,000 mg/kg. The analytical procedure was successfully adapted for quantitative determination of DC and CTC in medicated feedingstuff samples. Validation included determination of specificity, linearity, and repeatability. Mean recovery for spiked samples was 93.1% for CTC and 93.2% for DC. The results of validation of the analytical procedure proved that presented method is efficient, precise and useful for quantification of DC and CTC in medicated feedingstuffs.</p>
</abstract>

High performance liquid chromatography method with diode array detection (HPLC-DAD) was developed and optimised for the determination of tetracyclines (TCs) in medicated feedingstuffs. The extraction of the analyte from feedingstuffs was performed with Na2EDTA-McIlvaine buffer (pH 2.5 and pH 4). The extracts were cleaned up by solid phase extraction using octadecyl cartridges (C18). The samples were dried up and redissolved in the mixture of oxalic acid and methanol. Separation was performed on reserved phase column (Phenomenex C18, 250 x 4.6 mm, 5 μm) by multistep gradient elution, which provided better chromatographic separation. The analysis was performed at a wavelength of 390 nm. Validation study of the method revealed that all obtained calibration curves showed good linearity R= 0.9985 for doxycycline (DC) and R= 0.9981 for chlorotetracycline (CTC) over the range of 25-2,000 mg/kg. The analytical procedure was successfully adapted for quantitative determination of DC and CTC in medicated feedingstuff samples. Validation included determination of specificity, linearity, and repeatability. Mean recovery for spiked samples was 93.1% for CTC and 93.2% for DC. The results of validation of the analytical procedure proved that presented method is efficient, precise and useful for quantification of DC and CTC in medicated feedingstuffs.

Determination of Chlorotetracycline and Doxycycline in Medicated Feedingstuffs by Liquid Chromatography

Department of Hygiene of Feedingstuffs National Veterinary Research Institute

Journal of Veterinary Research

High performance liquid chromatography method with diode array detection (HPLC-DAD) was developed and optimised for the determination of tetracyclines (TCs) in...

Determination of Chlorotetracycline and Doxycycline in Medicated Feedingstuffs by Liquid Chromatography

Pubblicato online: 17 gen 2013

Pagine: 329 - 333

DOI: https://doi.org/10.2478/v10213-012-0058-5

Parole chiave
medicated feedingstuffs, doxycycline, chlorotetracycline, liquid chromatography

This content is open access.

Determination of Chlorotetracycline and Doxycycline in Medicated Feedingstuffs by Liquid Chromatography

Pubblicato online: 17 gen 2013

Pagine: 329 - 333

DOI: https://doi.org/10.2478/v10213-012-0058-5

Parole chiavemedicated feedingstuffs, doxycycline, chlorotetracycline, liquid chromatography

This content is open access.

Parole chiave
medicated feedingstuffs, doxycycline, chlorotetracycline, liquid chromatography