Twelve species of Mycetophilidae are recorded from the Krkonoˇe Mts as new to Bohemia or the entire Czech Republic. The material was obtained in the years 2006-2009 mainly by means of Malaise traps. Three species are new to the Czech Republic: Mycomya collini Edwards, 1941, Leia martinovskyi ˇevčík & Papp, 2003 and Pseudexechia tuomikoskii Kjærandsen, 2009.