<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Immune suppression resulting from chemoprophylaxis and potential drug interaction were investigated in experimental animals pre-medicated with ampicillin and chloroquine followed by immunization with bovine serum albumin bearing liposomes prepared by the reverse phase evaporation method. The prepared liposomes were evaluated for particle size, entrapment efficiency and <italic>in vitro</italic> release. Humoral immune response was measured in terms of systemic IgG antibody titre by the ELISA method. The present study showed that 7:3 molar ratio of soya phosphatidylcholine and cholesterol produced liposomes of mean diameter of 235.4 ± 10.3 nm and entrapment efficiency of 41.3 ± 3.2%. Ampicillin significantly (<italic>p</italic> &lt; 0.05) decreased the antibody titre whereas chloroquine did not reduce the antibody titre significantly. The study will help in programming a new drug management and in characterization of vaccine-drug interaction.</p></abstract>

Immune suppression resulting from chemoprophylaxis and potential drug interaction were investigated in experimental animals pre-medicated with ampicillin and chloroquine followed by immunization with bovine serum albumin bearing liposomes prepared by the reverse phase evaporation method. The prepared liposomes were evaluated for particle size, entrapment efficiency and in vitro release. Humoral immune response was measured in terms of systemic IgG antibody titre by the ELISA method. The present study showed that 7:3 molar ratio of soya phosphatidylcholine and cholesterol produced liposomes of mean diameter of 235.4 ± 10.3 nm and entrapment efficiency of 41.3 ± 3.2%. Ampicillin significantly (p < 0.05) decreased the antibody titre whereas chloroquine did not reduce the antibody titre significantly. The study will help in programming a new drug management and in characterization of vaccine-drug interaction.

Effect of ampicillin and chloroquine on humoral immune response elicited by bovine albumin encapsulated in liposomes

Department of Pharmaceutics, Hindu College of Pharmacy, Sonepat (Haryana), India

Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar (Haryana) India

Department of Pharmacy, Kumaon University, Nainital, India

Acta Pharmaceutica

Immune suppression resulting from chemoprophylaxis and potential drug interaction were investigated in experimental animals pre-medicated with ampicillin and...

Effect of ampicillin and chloroquine on humoral immune response elicited by bovine albumin encapsulated in liposomes

Pubblicato online: 22 dic 2008

Pagine: 479 - 487

DOI: https://doi.org/10.2478/v10007-008-0026-z

Parole chiave
imunomodulacija, liposomi, goveđi albumin, ampicilin, klorokin

This content is open access.

Effect of ampicillin and chloroquine on humoral immune response elicited by bovine albumin encapsulated in liposomes

Pubblicato online: 22 dic 2008

Pagine: 479 - 487

DOI: https://doi.org/10.2478/v10007-008-0026-z

Parole chiaveimunomodulacija, liposomi, goveđi albumin, ampicilin, klorokin

This content is open access.

Parole chiave
imunomodulacija, liposomi, goveđi albumin, ampicilin, klorokin